[PDF] Top 20 Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
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Identification and characterization of the herpes simplex virus type 1 protein encoded by the UL37 open reading frame.
... Protein extracts from the purified HSV-1 virions were separated on SDS-polyacrylamide gels, and the protein bands were probed in immunoblots with antisera directed against the UL37, ICP8[r] ... See full document
9
Identification of Proteins Phosphorylated Directly by the Us3 Protein Kinase Encoded by Herpes Simplex Virus 1
... where n is greater than or equal to 2, X can be Arg, Ala, Val, Pro, or Ser, and Y can be any residue except an acidic one (28). The predicted amino acid sequence of UL34 contains the con- sensus Us3 phosphorylation sites ... See full document
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The Herpes Simplex Virus Type 1 UL3 Transcript Starts within the UL3 Open Reading Frame and Encodes a 224-Amino-Acid Protein
... the protein-antibody complex ...FIG. 1. (A) Schematic diagram of the HSV-1 genome illustrating the relative sizes and locations of HSV-1 UL3-specific transcripts in the UL1-UL3 ...line ... See full document
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A Null Mutation in the Gene Encoding the Herpes Simplex Virus Type 1 UL37 Polypeptide Abrogates Virus Maturation
... the herpes simplex virus type 1 (HSV-1) ...The UL37 open reading frame of HSV-1 encodes a 120-kDa virion polypeptide which is a resident of the ... See full document
13
The unique sequence of the herpes simplex virus 1 L component contains an additional translated open reading frame designated UL49.5.
... This gene, consisting of the authentic promoter and chimeric coding domain, was inserted into the thymidine kinase gene of wild-type virus and in infected cells expressed a protein which[r] ... See full document
5
Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene.
... fusion protein was purified on glutathione beads, and a total of 10 mg of purified GST-ORF P fusion protein was inoculated into two ...repaired virus, or R7528 and main- tained at 378C were ... See full document
8
Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... UL56 open reading frame in bacteria as a glutathione-S-transferase (GST) fusion protein and prepared an HSV-2 UL56-specific anti- serum in rabbits to characterize the UL56 ...UL56 ... See full document
11
Varicella-zoster virus open reading frame 4 protein is functionally distinct from and does not complement its herpes simplex virus type 1 homolog, ICP27.
... 1987 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Varicella-zoster virus VZV open reading frame 4 ORF4 encodes a putative immediate-early protein which is homologous [r] ... See full document
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Cell lines containing varicella-zoster virus open reading frame 62 and expressing the "IE" 175 protein complement ICP4 mutants of herpes simplex virus type 1.
... Three neomycin-resistant cell lines were isolated and shown to complement two different ICP4 mutants tsB21 and d120 of herpes simplex virus HSV type 1 HSV-1.. VZV-specific RNA could not [r] ... See full document
7
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene.
... genes encoded by herpes simplex virus type 1 (HSV-1) ...virion protein VP16, which, in conjunction with cellular proteins, binds to it and transactivates the ... See full document
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Localization of Herpes Simplex Virus Type 1 UL37 in the Golgi Complex Requires UL36 but Not Capsid Structures
... the UL37 gene did not affect the function of the protein, in that a virus specifying this insertion was easily isolated and displayed rep- lication properties similar to those of the wild-type ... See full document
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Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.
... The 12-kDa protein recognized by the TrpE-UL35 antiserum was associated with purified HSV-1 virions and type A and B capsids, suggesting that the UL35 ORF may encode the 12-kDa capsid pr[r] ... See full document
11
Identification, Localization, and Regulation of Expression of the UL24 Protein of Herpes Simplex Virus Type 1
... of herpes simplex virus type 1 is conserved across many herpesviruses, but its protein product has not been ...resulting protein to raise a rat ...30-kDa protein in ... See full document
8
Prediction and Identification of Herpes Simplex Virus 1-Encoded MicroRNAs
... At present, there are few genetic clues for biological activ- ities of HSV miRNAs. The DNA sequence encoding HSV-1 pre-miR-H1 spans the 5 ⬘ untranslated region and the open reading frame of ... See full document
10
Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ... See full document
12
Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... 18 h after infection, monolayers were washed once with PBS(A), scraped, pel- leted by centrifugation, resuspended in 1 mM phosphate buffer (pH 7.4), and disrupted by 20 strokes of Dounce homogenization. The sample ... See full document
9
Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
... herpesvirus 1 (21), were reported and reviewed (55, ...novel open reading frames, highlighted the presence of various cellular components in the virions, and examined virulence ...⬍ 1% of ... See full document
14
Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0.
... Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICPO exhibit impaired growth in cell culture.. GAL4-VP16 is an unusually potent transcriptio[r] ... See full document
6
The Capsid Protein Encoded by UL17 of Herpes Simplex Virus 1 Interacts with Tegument Protein VP13/14
... ⫹ 1 insertion mutation in HSV-1(F) U L 17 was predicted to cause a frame- shift relative to HSV-1(17) at codon 11 ...The open reading frames then realign at codon 21 by a ... See full document
9
Herpes simplex virus 1 protein kinase is encoded by open reading frame US3 which is not essential for virus growth in cell culture.
... Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 606372 Received 16 April 1987/Accepted 11 June 1987 We report that a novel protein kinase previously shown to[r] ... See full document
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