[PDF] Top 20 Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
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Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document
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Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.
... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document
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Physical and functional domains of the herpes simplex virus transcriptional regulatory protein ICP4.
... Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.. Activation of immediate-early, early, and[r] ... See full document
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Initial characterization of the membrane-associated form of ICP4 of herpes simplex virus type 1.
... The immediate-early gene product, ICP4, of herpes simplex virus type 1 (HSV-1) is one of the major transcriptional regulatory proteins in the ... See full document
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Overexpression of the herpes simplex virus type 1 immediate-early regulatory protein, ICP27, is responsible for the aberrant localization of ICP0 and mutant forms of ICP4 in ICP4 mutant virus-infected cells.
... of ICP4, in which the staining pattern of ICP0 was not changed in the presence of mutant ICP4 ...of ICP4 ts mutant viruses at the NPT showed that both ICP0 and ts forms of ICP4 were localized ... See full document
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The herpes simplex virus immediate-early protein, ICP4, is required to potentiate replication of human immunodeficiency virus in CD4+ lymphocytes.
... Deletion mutants in the gene encoding the herpes simplex virus type I immediate-early protein ICPO exhibit impaired growth in cell culture.. The herpes simplex virus type 1 alpha protein[r] ... See full document
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Cross-reactivity of the anti-pml antibody PG-M3 with the herpes simplex virus type 1 immediate early protein ICP4
... kDa protein species in these experiments ...HSV-1 immediate early protein ICP4, a known component of replication compartments (DeLuca & Schaffer, ...sequences encoding ... See full document
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Repression of gene expression upon infection of cells with herpes simplex virus type 1 mutants impaired for immediate-early protein synthesis.
... of gene expression which, at the experimental level, masks events in cells containing quiescent ...IE gene expression even further have been devised. Virus in1820 is in1814 with the ICP0 promoter ... See full document
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Prolonged gene expression and cell survival after infection by a herpes simplex virus mutant defective in the immediate-early genes encoding ICP4, ICP27, and ICP22.
... Very early in infection, herpes simplex virus (HSV) expresses four immediate-early (IE) regulatory proteins, ICP4, ICP0, ICP22, and ...resulting mutants, ... See full document
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Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICP0 exhibit impaired growth in cell culture.
... These mutants contain wild-type copies of ICPO yet fail to express early and Of the three major kinetic classes of genes of herpes simplex virus type 1 HSV-1, the immediate-early a genes[r] ... See full document
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Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression.
... Isolation and characterisation of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide VmwllO.. The DNA-binding domains of [r] ... See full document
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Binding of ICP4, TATA-Binding Protein, and RNA Polymerase II to Herpes Simplex Virus Type 1 Immediate-Early, Early, and Late Promoters in Virus-Infected Cells
... of ICP4, TBP, and polII to promoters as a func- tion of ICP4 functional ...of ICP4, TBP, and polII to promoters as a function of cis-acting sites within the ...that ICP4 is required for the ... See full document
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Evidence that the Immediate-Early Gene Product ICP4 Is Necessary for the Genome of the Herpes Simplex Virus Type 1 ICP4 Deletion Mutant Strain d120 To Circularize in Infected Cells
... novo protein synthesis, a noticeable amount of input KOS genomes circularized in the presence of CHX in both Vero and E5 cells ...novo protein synthesis is not needed for the wild-type HSV-1 ... See full document
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Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175.
... Consistent with this model are the following observations: i certain late proteins e.g., VP154 can be expressed in the absence of functional VP175 this study, ii VP175 is required for ce[r] ... See full document
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Herpes simplex virus type 1 ICP27 is an essential regulatory protein.
... We report herein the genetic and phenotypic characterization of four temperature-sensitive mutants of herpes simplex virus type 1 tsY46, tsE5, tsE6, and tsLG4 that have begun to elucidat[r] ... See full document
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Association of a major transcriptional regulatory protein, ICP4, of herpes simplex virus type 1 with the plasma membrane of virus-infected cells.
... When identical samples were immunoblotted with anti-ICP4 serum, significant amounts of ICP4 were present in the purified plasma membrane fraction, suggesting that ICP4 was associated wit[r] ... See full document
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Control of VP16 Translation by the Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27
... A protein termed eIF4AIII, which is highly similar to translation initiation factors eIFAI and eIFAII, has been shown to be an integral part of the EJC (2, 13, 53, ...shuttling protein, interacts with Y14 ... See full document
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ICP4, the major transcriptional regulatory protein of herpes simplex virus type 1, forms a tripartite complex with TATA-binding protein and TFIIB.
... Specifically, the simultaneous addition of ICP4, rTFIIB, and rTBP to a DNA template containing an ICP4 binding site and a TATA box resulted in a unique complex lower in electrophoretic m[r] ... See full document
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Isolation of novel herpes simplex virus type 1 derivatives with tandem duplications of DNA sequences encoding immediate-early mRNA-5 and an origin of replication.
... The other isolate, SP22-4, carried a 3.5-kilobase-pair duplication defined by a recombination event between a tandem repeat array in the BamHI Z fragment and a site near the amino termin[r] ... See full document
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Identification of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... 818 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hybrid arrest of in vitro translation was used to localize the region of the herpes simplex virus type 1 genome enco[r] ... See full document
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