[PDF] Top 20 Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.

... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document

... Since SV40 DNA replication absolutely depends on SV40 T antigen, anti-T serum from a tumor-bearing hamster was added to the reaction mixture containing both HeLa nuclear extract and CosI[r] ... See full document

... AcMNPV, DNA replication in simian virus 40 (SV40) has been extensively ...The virus possesses just one origin and encodes only a single protein involved in the replication ... See full document

... of T antigen was then performed to define the site of interaction more ...to T- antigen residues 1 to 249 but poorly to 1 to 83 and 1 to 147 ...bearing T-antigen residues ... See full document

... of simian virus 40 (SV40) large T antigen play a critical role in DNA ...of mutants (class A: D429A, N449S, and N515S) with normal DNA binding and ATPase and ... See full document

... A mutants except that they demonstrated better ssDNA bind- ing activity than did WT T antigen, especially at low protein ...the large tier ...WT T antigen, the three residues, ... See full document

... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ... See full document

... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...deletion mutants, we show that this domain most likely begins after residue 301 and that the ... See full document

... they define the nature of mitotic and DNA damage defects caused by LT that affect genome ...between replication stress, DNA dam- age, and mitotic ...induce replication stress, leading ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document

... Simian virus 40 (SV40) large tumor (T) antigen is the major regulatory protein that directs the course of viral infection, primarily by interacting with host cell proteins and ... See full document

... dependent replication in vivo, we conversely demonstrated that FancD2 and Rad51 play important ...episomal replication with high fidelity might require the HR machinery to repair damage to the viral genome, ... See full document

... 3 0022-538X/83/090487-08$02.00/0 Copyright © 1983, American Society for Microbiology Nonviable Mutants of Simian Virus 40 with Deletions Near the 3' End of Gene A Define a Function for L[r] ... See full document

... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ... See full document

... Eukaryotic DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance ... See full document

... Several T-antigen activities, such as origin binding, viral DNA replication, ATPase activity, stimulation of cell DNA synthesis, stimulation of rDNA transcription, transformation, and nu[r] ... See full document

... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document

... 5031 T antigens are in fact sufficient to mediate priming by pol-prim on RPA-coated ssDNA but that 5030 and 5031 T antigens differ in their ability to functionally coordinate an RPA–pol-prim ...the ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... It also plays central roles in immortalization of primary cells 61, 62 and in the malignant Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Linker insertion mutants aff[r] ... See full document