[PDF] Top 20 Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication.

... On the other hand, aspartic acid substitution of serines 257 and 259 caused specific reductions in enveloped virus production, suggesting that phosphorylation at these sites would be exp[r] ... See full document

... detect capsid rephosphorylation during virus entry may reflect the possibility that this process occurs at an earlier ...as hepatitis B virus and human immunodeficiency virus, ... See full document

... by phosphorylation) but were restored to baseline levels at 24 ...simian virus 40 promoter in NIH 3T3 cells or the cytomegalovirus promoter in HepG2 cells, evaluated at 48 h after induction ...FAK ... See full document

... Much attention was focused on the C-terminal region of this protein, in which several domains have been found to mediate different functions in the viral life cycle; e.g., the C-terminal[r] ... See full document

... P protein binding to the ⑀ RNA ...overall replication capacity of most ...like replication, was more tolerant to the mutations L559I and L559M than to the mutation L559A, and the F558A variant ... See full document

... versus replication-competent (packaging the pgRNA-RT complex) capsids ...CTD phosphorylation and ...of phosphorylation is proposed to be regulated dynamically by the host protein kinase(s) and ... See full document

... enzymatic functions, some transcriptional coactivators, such as the peroxisome proliferator-activated receptor ␥ coactivator 1 (PGC1) family members PGC1 ␣ , PGC1 ␤ , and peroxisome proliferator-activated receptor ... See full document

... Removal of the last 12 C-terminal DHBcAg amino acids, which are at least in part located on the core particle surface, had only a minor effect on DHBcAg phosphorylation and did not inter[r] ... See full document

... Phosphorylation at these four sites resulted in complex shifts in electrophoretic mobility in sodium dodecyl sulfate gels of the capsid protein or of a C-terminal peptide containing the [r] ... See full document

... core protein phosphorylation ...core protein mutant in common, the serine 155 mutant, ...core protein C-terminal mutants displayed an altered capsid conformation, as detected by ... See full document

... that phosphorylation of NS5A may play a role in regulating its differential functions at different stages of the virus lifecycle, for example effecting a switch between genome replication and ... See full document

... of duck hepatitis B virus, woodchuck hepatitis virus, and ground squirrel hepatitis virus (6, 9, ...DNA, phosphorylation has been implicated in modulation of ... See full document

... X protein (HBx), a multifunctional regulatory protein, plays a pivotal role in viral liver pathogenesis (2) and especially in HBV replication (3, ...viral replication and HBV-induced liver ... See full document

... complete replication- competent DHBV genome as present in plasmid pCD16 (care had been taken during construction of the GGE mutations in the DHBc ORF that these did not cause any amino acid changes in the ... See full document

... (hepatitis B viruses [HBVs]) are a fam- ily of small, enveloped DNA viruses comprising the medically important human HBV and other members infecting rodents and birds ...unique replication strategy ... See full document

... the protein-priming reaction for minus strand DNA synthesis (25), it is likely that we isolated capsids that had already completed the protein-priming reac- tion and contained short minus ...to ... See full document

... expressed protein spots between DHBV infected and uninfected PDHs, were excised, digested in- gel with trypsin and determined by ...expressed protein spots, 51 protein spots were identified ... See full document

... In summary, we showed that F122V, F122Y, F122S, R127D, R127G, and possibly R98H mutations allowed the formation of intracellular capsids, with the F122V level even close to WT levels, but strongly reduced the ... See full document

... MASON Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Analysis of duck hepatitis B viral DNA by gel electrophoresis, Southern blotting, and bindi[r] ... See full document

... core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of ... See full document