[PDF] Top 20 Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.
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Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.
... wild type. This agrees with our results obtained with the dS.1 and DSL1 mutants and suggests that interstrand base pairing in the SL1 region contributes little to the overall stability of mature ... See full document
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Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization.
... of human immunodeficiency virus type 1 (HIV-1) and chimpanzee immunodeficiency virus genomic ...vitro dimerization of short HIV-1 RNA ... See full document
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Maintenance of the Gag/Gag-Pol Ratio Is Important for Human Immunodeficiency Virus Type 1 RNA Dimerization and Viral Infectivity
... the human immunodeficiency virus type 1 (HIV-1) Gag-Pol precursor protein results from a ⴚ 1 ribosomal frameshifting ...in virus-producing cells by cotransfecting ... See full document
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Requirements for kissing-loop-mediated dimerization of human immunodeficiency virus RNA.
... viral RNA strands is proposed to occur initially at a conserved, six-base, GC-rich palindrome in the SL1 loop (13, 19, ...SIV genomes (GUGCAC) also conforms to this ...identical RNA strands be com- ... See full document
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Effects of nucleocapsid mutations on human immunodeficiency virus assembly and RNA encapsidation.
... The mutant sequences are as follows, where the 59 and 39 proviral nucleotide numbers are provided, wt HIV sequences are in normal type, oligonucleotides are in boldface type, and the termination ... See full document
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Efficient encapsidation of human immunodeficiency virus type 1 vectors and further characterization of cis elements required for encapsidation.
... vector encapsidation, we first created an HIV-1 vector by disrupting translation of Gag mRNA at a location distal to cis elements known to be essen- tial for encapsidation ...the mutant env ... See full document
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Human Immunodeficiency Virus Types 1 and 2 Differ in the Predominant Mechanism Used for Selection of Genomic RNA for Encapsidation
... of encapsidation of coexpressed vector and helper constructs and thus have the benefit of controlling for intersample ...wild-type virus as an internal control, and cytoplasmic and virion RNAs were ... See full document
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Dissociation of Genome Dimerization from Packaging Functions and Virion Maturation of Human Immunodeficiency Virus Type 1
... wild type (see ...its RNA, since the infectivities of other mutants that showed various RNA profiles also displayed the same magnitude of ...viral RNA could be the template for reverse ... See full document
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An Extended Stem-Loop 1 Is Necessary for Human Immunodeficiency Virus Type 2 Replication and Affects Genomic RNA Encapsidation
... in virus replication in cell ...weaker RNA-RNA duplexes (compared to the wt sequence) in vitro ...pal mutant viruses did not lead to productive ...G394A/T397C mutant pal 392-401 regions ... See full document
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Human immunodeficiency virus type 1 RNA outside the primary encapsidation and dimer linkage region affects RNA dimer stability in vivo.
... in encapsidation compared to WT RNA (35, ...genomic RNA is packaged in these mutants, we thought it would be feasible to compare the stability of those mutant dimeric RNAs with that of ... See full document
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The Double-Stranded RNA-Binding Protein Staufen Is Incorporated in Human Immunodeficiency Virus Type 1: Evidence for a Role in Genomic RNA Encapsidation
... genomic RNA in retroviruses (33, ...two RNA dimers ...the RNA molecules tested were shorter than the genomic ...an RNA of ⬇20 kb, over two times the wild-type size (68), was shown to be ... See full document
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Duplication of the Primary Encapsidation and Dimer Linkage Region of Human Immunodeficiency Virus Type 1 RNA Results in the Appearance of Monomeric RNA in Virions
... two RNA molecules is noncovalent because the dimeric RNA dissociable into monomers under mild denaturing conditions, such as incubation at high temper- ature (⬃70°C) or treatment with denaturing reagents ... See full document
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RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant
... sarcoma virus (RSV), murine leukemia virus, human immunodeficiency virus type 1, and human T-cell leukemia virus type 1 that lack infectivity ... See full document
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Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... GA-rich RNA sequences at nt 325 to 336 sig- nificantly reduce levels of both viral RNA dimerization and ...GA-rich RNA sequence downstream of the SL3 structure participates in viral RNA ... See full document
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Distinct functions and requirements for the Cys-His boxes of the human immunodeficiency virus type 1 nucleocapsid protein during RNA encapsidation and replication.
... during RNA recognition and encapsidation is ...genomic RNA may be mediated through a single Gag mono- mer or by multiple Gag ...monomers. Virus particles produced by transfection of WT-to-NC ... See full document
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Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2.
... viral RNA analysis from one such ...to RNA, half of each sam- ple was treated with NaOH (2 M for 15 min at 658C) prior to the slot blot ...genomic RNA at approximately 50% of the wild-type ... See full document
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A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... Genomic RNA was packaged at similar levels in wild-type and mutant virions produced by transfected Cos7 ...mic RNA packaging was found to be decreased from twofold (substitution mutants) to ... See full document
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Binding of the Human Immunodeficiency Virus Type 1 Gag Protein to the Viral RNA Encapsidation Signal in the Yeast Three-Hybrid System
... cognate RNA encapsidation signal that reflects the interac- tions seen in vitro and in ...of RNA or protein for the ability to interact. Mutant Gags or RNAs with lower or higher binding ... See full document
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Possible Role of Dimerization in Human Immunodeficiency Virus Type 1 Genome RNA Packaging
... The RNA profile from dLB3-BA showed that the intro- duced deletion had an intermediate effect on dimer formation ...total RNA of the virion produced from DDNBA varied slightly from experiment to experiment, ... See full document
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Impact of Human Immunodeficiency Virus Type 1 RNA Dimerization on Viral Infectivity and of Stem-Loop B on RNA Dimerization and Reverse Transcription and Dissociation of Dimerization from Packaging
... lower encapsidation, it was not clear that RU5 DNA production was decreased (pR-pU5⬘ column of Table 1), and PBS-MA DNA production was not dramati- cally reduced (pPBS-pMA⬘ column of Table 1), ... See full document
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