[PDF] Top 20 Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
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Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... mutant T antigens were assayed for the ability to relieve the RPA-mediated inhibition of primer synthesis and elongation (12, ...pol-prim, T antigen, unlabeled ribo- and deoxyribonucleoside ... See full document
10
Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... of simian virus 40 (SV40) large T antigen play a critical role in DNA ...normal DNA binding and ATPase and helicase activities but with a severely reduced ability ... See full document
11
Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... large T antigen (T-ag) is a multifunctional protein that successively binds to 5 ⴕ -GAGGC-3 ⴕ sequences in the viral origin of replication, melts the origin, unwinds DNA ahead of ... See full document
13
Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... for T-ag assembly events, we analyzed binding to larger subfragments of the core ...support T-ag assem- bly, albeit at lower levels compared to wild-type sequences, indicates that both sequence-specific and ... See full document
13
Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... of viral DNA replication, we examined the relationship between T-antigen oligomeric state and DNA helicase ...of T antigen were incubated with a model DNA ... See full document
8
Simian virus 40-transformed human cells that express large T antigens defective for viral DNA replication.
... Although the fragment encoded a full-length viral early region and could induce T antigen effectively in CV-1 cells, its replication was only detected when transfected into COS-7 cells, [r] ... See full document
13
Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.
... 66, 1992 ROLE OF T ANTIGEN AS A REPLICATIVE DNA HELICASE the viral origin of DNA replication, a bilobed T-antigen helicase complex was observed operating simultaneously at both forks of [r] ... See full document
12
Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.
... To investigate the specificity of pseudorevertant T antigens for different ori sequences, we constructed in vitro recombinants that contained mutant T antigen genes and various sequences[r] ... See full document
8
The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication
... the T-antigen region responsible for physical interaction with RPA and to verify its relevance in the functional interactions between these proteins in the replication of SV40 ...the DNA ... See full document
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Simian Virus 40 DNA Replication Is Dependent on an Interaction between Topoisomerase I and the C-Terminal End of T Antigen
... in DNA replication activity ...near-normal DNA replication activity were stimulated by topo I just like the WT ...of DNA replication and topo I binding activities of ... See full document
10
Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...support simian virus 40 (SV40) replication and to bind to single-stranded ...Two ... See full document
9
Thermally inactivated simian virus 40 tsA58 mutant T antigen cannot initiate viral DNA replication in vitro.
... Reactions containing SV40 T antigen T-Ag or tsA58 T antigen were preheated for 30 min at 41°C in the absence of ATP, in the presence of 2.5 mM AMP-PNP, or in the presence of 2.5 mM ATP u[r] ... See full document
12
A Structure-Guided Mutational Analysis of Simian Virus 40 Large T Antigen: Identification of Surface Residues Required for Viral Replication and Transformation
... both T antigen and p53 (29). The functional consequences of T antigen acetylation are as yet ...wild-type T antigen, ostensibly a conse- quence of the weakened interaction ... See full document
8
Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... many activities including binding ssDNA and origin DNA at low protein concentrations, helicase activity, unwinding of the or- igin, and supporting DNA ... See full document
10
Simian virus 40 small-t antigen stimulates viral DNA replication in permissive monkey cells.
... To explore this apparent paradox between in vivo and in vitro experiments, we examined the effect of small t on replication of microinjected viral DNA and the effects of small-t antigen [r] ... See full document
7
Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.
... 411 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Various monoclonal antibodies specific for simian virus 40 large tumor antigen T antigen inhibit the elongation proc[r] ... See full document
8
Structural topography of simian virus 40 DNA replication.
... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ... See full document
11
Analysis of Simian Virus 40 Chromosome—T-Antigen Complexes: T-Antigen Is Preferentially Associated with Early Replicating DNA Intermediates
... 281-295 0022-538X/83/100281-15S02.00/0 Copyright © 1983, American Society for Microbiology Analysis of Simian Virus 40 Chromosome-T-Antigen Complexes: T-Antigen Is Preferentially Associa[r] ... See full document
15
Differential induction of structural changes in the simian virus 40 origin of replication by T antigen.
... 1228 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The ATP-dependent binding of the simian virus 40 SV40 large tumor antigen T antigen to the SV40 origin of replicati[r] ... See full document
8
ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ... See full document
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