[PDF] Top 20 Mutations of basic amino acids of NCp7 of human immunodeficiency virus type 1 affect RNA binding in vitro.
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Mutations of basic amino acids of NCp7 of human immunodeficiency virus type 1 affect RNA binding in vitro.
... of human immunodeficiency virus type 1 is required for packaging of viral RNA and for virion ...of basic amino acids, consisting of five and four ... See full document
7
Mutations Proximal to the Minor Groove-Binding Track of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Differentially Affect Utilization of RNA versus DNA as Template
... and RNA tem- plates, some amino acid residues may need to interact with both types of nucleic acids, whereas others may be specific to ...the RNA strand, at least six amino acid ... See full document
9
Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... (Fig. 1) (2, ...in vitro (9, 10, 11, 12, 13, 14, 15, 26, 27, ...the RNA strand) the RNase H active ...RSV, mutations in U5 did not affect the specificity of the cleavage that removes ... See full document
9
Cryoelectron Microscopic Examination of Human Immunodeficiency Virus Type 1 Virions with Mutations in the Cyclophilin A Binding Loop
... and 1-kDa proteins (17, 18, 25, 41). Maturation of human immunodeficiency virus type 1 (HIV-1) particles involves condensation of the capsid protein around the ... See full document
5
Effect of mutations in the nucleocapsid protein (NCp7) upon Pr160(gag-pol) and tRNA(Lys) incorporation into human immunodeficiency virus type 1.
... and basic amino acid residues and whose sequences are always found C terminal to the matrix and capsid domains in the Gag and Gag-Pol ...and RNA packaging, see references 3, 11, and ...genomic ... See full document
7
Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly
... by RNA-binding ...bind RNA. To deter- mine if RNA binding could restore particle production in the Gag double mutant, we inserted potential RNA-binding sequences into the ... See full document
9
Hydrophobic Amino Acids in the Human Immunodeficiency Virus Type 1 p2 and Nucleocapsid Proteins Can Contribute to the Rescue of Deleted Viral RNA Packaging Signals
... in virus particles despite the lower levels of BH-DG RNA in cells ...BH-DG RNA was more efficiently packaged into virus particles than ⌬DIS RNA; in contrast, the MP2 and MNC point ... See full document
14
Basic Residues in Human Immunodeficiency Virus Type 1 Nucleocapsid Promote Virion Assembly via Interaction with RNA
... NC mutations were associated with decreased processing of the Gag polyprotein by the viral ...of virus-producing cells by electron microscopy showed that a higher proportion of mutant virions exhibit ... See full document
12
Roles of Gag and NCp7 in Facilitating Annealing to Viral RNA in Human Immunodeficiency Virus Type 1
... the basic amino acids flanking the first zinc ...double-stranded RNA regions in viral RNA involved in annealing (such as the primer activation signal), and/or alter RNA ... See full document
9
Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site.
... the RNA template must be prerequisite for correct annealing between these struc- tures and in order for efficient reverse transcription to ...viral RNA and the tRNA ...highly basic and contains one ... See full document
9
Amino-Terminal Substitutions in the CCR5 Coreceptor Impair gp120 Binding and Human Immunodeficiency Virus Type 1 Entry
... the amino terminus (Nt) and three extracellular loops (ECL1 to ECL3) ...the amino termini of other 7TM receptors, have been previously implicated in binding of peptide ligands (12, 29, 31, 42) and ... See full document
7
Mutations That Abrogate Human Immunodeficiency Virus Type 1 Reverse Transcriptase Dimerization Affect Maturation of the Reverse Transcriptase Heterodimer
... wild-type virus for the HX-W401L and HX-W401A ...the virus, allowing cleavage of the RNase H domain from one of the p66 monomers to ...inhibitors ... See full document
11
In Vitro Suppression of Human Immunodeficiency Virus Type 1 Replication by Measles Virus
... measles, human immunodeficiency virus type 1 (HIV-1)-infected children have a transient, but dramatic, decrease in plasma HIV-1 RNA levels ...measles virus ... See full document
9
Functional tolerance of the human immunodeficiency virus type 1 envelope signal peptide to mutations in the amino-terminal and hydrophobic regions.
... 5114-5118 0022-538X/92/085114-05$02.00/0 Copyright 1992, American Society for Microbiology Functional Tolerance of the Human Immunodeficiency Virus Type 1 Envelope Signal Peptide to Mut[r] ... See full document
5
Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations.
... Making the entire viral DNA portion single-stranded further reduced the disintegration activity approximately twofold observed with the wild type and mutants ING47L, DISCUSSION We expres[r] ... See full document
13
Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro.
... 6361-6369 0022-538X/92/116361-09$02.00/0 Identification of Conserved Amino Acid Residues Critical for Human Immunodeficiency Virus Type 1 Integrase Function In Vitro ALAN ENGELMAN AND RO[r] ... See full document
9
Determinants of the human immunodeficiency virus type 1 p15NC-RNA interaction that affect enhanced cleavage by the viral protease.
... wild type in cells and in released virions, and precursor was evident even after a 5-h chase ...CS2 mutations on the rate of cleavage of p15NC, both mutations resulted in a small delay in cleavage at ... See full document
10
Roles of Pr55gag and NCp7 in tRNA3Lys Genomic Placement and the Initiation Step of Reverse Transcription in Human Immunodeficiency Virus Type 1
... Viral RNA annealing activities of human immuno- deficiency virus type 1 nucleocapsid protein require only peptide domains outside the zinc ... See full document
5
Human Immunodeficiency Virus Type 1 Spinoculation Enhances Infection through Virus Binding
... of virus and cells does not overcome the diffusion-imposed limitations to infection that are operative at ambient gravity ...at 1 ⫻ g (i.e., the distance that a virus is expected to move by diffusion ... See full document
7
Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization.
... 1875-1883 0022-538X/92/041875-09$02.00/0 Copyright C 1992, American Society for Microbiology Mutations in the Principal Neutralization Determinant of Human Immunodeficiency Virus Type 1 [r] ... See full document
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