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[PDF] Top 20 Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections

Has 10000 "Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections" found on our website. Below are the top 20 most common "Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections".

Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections

Rapid Real Time Nucleic Acid Sequence Based Amplification Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections

... the time blood bottles were flagged as positive by the automated blood culture systems and processed for routine microbiologic ...the time of ...for real-time NASBA ...each ... See full document

12

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp  in Respiratory Specimens

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens

... isothermal nucleic acid sequence-based amplification (NASBA) was developed to detect Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella ...For ... See full document

7

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St  Louis Encephalitis Viruses

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses

... NASBA amplification with real-time molecular beacon detection of dilutions of WN NY 1999 ...The amplification plot was generated in an ABI Prism 7700 sequence detection ... See full document

8

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

... a rapid NASBA-based viability ...a rapid viability assay would be useful include Legionella, Listeria, Campylobacter, ...to nucleic acids extracted directly from the food ...Biosensor ... See full document

16

Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites

Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites

... QT-NASBA amplification was detected by ECL, which makes the assay more laborious and ...a molecular beacon as a fluorescent label, a real-time QT-NASBA format can be constructed ...This ... See full document

6

Duplex DNA Invading γ Modified Peptide Nucleic Acids Enable Rapid Identification of Bloodstream Infections in Whole Blood

Duplex DNA Invading γ Modified Peptide Nucleic Acids Enable Rapid Identification of Bloodstream Infections in Whole Blood

... could detect and discriminate a broad pathogen panel, thus max- imizing its clinical ...and sequence selectivity on the analyte detection ...turnaround time to under ...reported molecular ... See full document

11

Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real Time Nucleic Acid Sequence Based Amplification

Detection of Aspergillus fumigatus in a Rat Model of Invasive Pulmonary Aspergillosis by Real Time Nucleic Acid Sequence Based Amplification

... A real-time nucleic acid sequence-based amplification (NASBA) method was investigated by use of an inhalational rat model of ...Total nucleic acid (TNA) was ... See full document

6

Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

... is based on microscopic detection of Plasmodium parasites in Giemsa-stained blood ...and molecular detection assays were introduced as alternatives to microscopy ...identify infections caused by ... See full document

6

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

... for amplification. Primer sets and molecular beacon sequences used for simplex and duplex NASBA reactions are shown in Table ...gyrB molecular beacons were used. To obtain equal ... See full document

5

Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia

Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia

... probes. Real-time monitored NASBA (9) was used for the detection of HIV-1-derived RNAs and for genotype ...isothermal amplification reaction mixture, and the amplification was performed in a ... See full document

8

Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection

Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection

... The real-time quantitative test for the detection of HBV that we have developed is easy to perform and saves time, due to a reduction of handling ... See full document

10

Diagnosing Herpesvirus Infections by Real Time Amplification and Rapid Culture

Diagnosing Herpesvirus Infections by Real Time Amplification and Rapid Culture

... using real-time technique were developed to demonstrate the presence of herpes simplex virus type 1 (HSV-1) and HSV-2, varicella zoster virus (VZV), and cytomegalovirus (CMV) in miscellaneous clinical ...to ... See full document

5

RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples

RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples

... (Baker), 5 mM dithiothreitol (Sigma), 15% (vol/vol) dimethyl sulfoxide (DMSO; Sigma), 1 mM (each) deoxynucleoside triphosphate (dNTP; Pharmacia), 2 mM rATP, rUTP, and rCTP and 1.5 mM rGTP (Pharmacia), 0.5 mM ITP (Boehr- ... See full document

7

Construction Strategy for an Internal Amplification Control for Real Time Diagnostic Assays Using Nucleic Acid Sequence Based Amplification: Development and Clinical Application

Construction Strategy for an Internal Amplification Control for Real Time Diagnostic Assays Using Nucleic Acid Sequence Based Amplification: Development and Clinical Application

... nalidixic acid, and vancomycin (Becton Dickinson and ...shaking. Nucleic acids were obtained by using the isolation module of the NucliSens basic kit (bioMe ´rieux bv, Boxtel, The Netherlands), according to ... See full document

6

Rapid and sensitive real time assay for the detection of respiratory syncytial virus using RT SIBA®

Rapid and sensitive real time assay for the detection of respiratory syncytial virus using RT SIBA®

... The assay was designed to detect all known human RSV strains including subtypes A and B [14]. RSV sequences were retrieved from the Virus Pathogen Resource [16] as well as the National Center for Biotechnology ... See full document

7

Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season

Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season

... The third portion was mixed with an equal volume of minimum essential medium containing gentamicin, 50 ␮ g/ml (Gibco, Paisley, United Kingdom), and vortexed with glass beads for 1 min; 200 ␮l was inoculated into ... See full document

6

Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets

Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets

... Alternatively, nucleic acid sequence-based amplification (NASBA) is faster than RT- PCR for RNA amplification and compares favorably with RT-PCR for RNA detection of other ... See full document

7

Improved Detection of Rhinoviruses by Nucleic Acid Sequence Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains

Improved Detection of Rhinoviruses by Nucleic Acid Sequence Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains

... Typing of RV clinical isolates. NASBA using primer mix 1 and probe combinations I and III classified 80 of 88 (90.9%) of clinical isolates as group B RVs. Primer mix 3 and probe combination IV allowed the classification ... See full document

6

Molecular Detection of Culture Confirmed Bacterial Bloodstream Infections with Limited Enrichment Time

Molecular Detection of Culture Confirmed Bacterial Bloodstream Infections with Limited Enrichment Time

... to detect six culture-confirmed cases (all GNRs) by both the universal and, if applicable, the enteric-bacterium-specific ...purified bacterial isolates (data not ...these time point aliquots (data ... See full document

6

Comparison of RNA and DNA-Based Amplification Methods for the Discrimination of Viable from Non-Viable Salmonella Typhimurium

Comparison of RNA and DNA-Based Amplification Methods for the Discrimination of Viable from Non-Viable Salmonella Typhimurium

... nalidixic acid or chloramphenicol (as evidenced by the absence of growth on CCDA plates), a positive NASBA-ELGA signal was detected when extracted rRNA was amplified immediately after each treatment; however, the ... See full document

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