[PDF] Top 20 Perturbation of Cell Cycle Progression and Cellular Gene Expression as a Function of Herpes Simplex Virus ICP0

... the expression ICP0 can have profound effects on the metabolism and survival of ...expressing ICP0 can remain metabolically active for prolonged periods of time, they are growth arrested and ... See full document

... between cell types and inducers and use multiple sig- naling ...HSV gene products may be sufficient for this activity (45, ...such gene product is ICP0. Therefore, ICP0 expressed very ... See full document

... of ICP0 is a dynamic process (1, 80), the role of phos- phorylation in ICP0 function, the location of specific phos- phorylation sites on ICP0, and the kinases that phosphorylate ICP0 ... See full document

... with ICP0 contained in HSV-1-infected cell ...the expression of cyclin D3, the gene was inserted into the viral genome and overexpressed in infected ...with ICP0 in nuclear structures ... See full document

... of ICP0 in the growth and replication of HSV-1 in low-multiplicity infections was revealed by analysis of the growth kinetics of a0 mutant ...a0 gene (vCM2/7, vC116G, vC116G/ C156A, and dl1403) exhibited ... See full document

... these cellular responses to effi- ciently replicate and ...the expression of immunomodulatory ...and cellular innate signaling components to counteract intrinsic and innate immune responses, viral ... See full document

... high-level expression of PML has no detrimental effect on HSV-1 infection (6, 23), and the replication of neither wild-type (wt) nor ICP0-negative HSV-1 viruses is improved in mouse PML ⫺/⫺ fibroblasts ... See full document

... The herpes simplex virus type 1 (HSV-1) immediate-early (IE) protein ICP0 (Vmw110) is a RING finger protein en- coded by IE gene 1 and is a strong and promiscuous activator of ... See full document

... Herpes simplex virus type 1 (HSV-1) ICP0 is required for efficient viral gene expression during lytic infection, especially at low ...of cellular activities that can ... See full document

... of ICP0 null mutants is highly multiplicity de- pendent (7, 8, 18, ...tion, gene expression and replication are severely impaired in ICP0 null mutant-infected cells relative to wild-type ... See full document

... wild-type virus-infected cells correlates with reduced p21 levels. One function of activated cdk4 is to phosphorylate ...allowing cycle progression (5, 28, ...recombinant virus R7914 or ... See full document

... infected cell protein 0 (ICP0), an ␣ protein encoded by herpes simplex virus 1 (HSV-1), interacts with many diverse cellular proteins and performs multiple functions ...ons—a ... See full document

... influence cellular processes is needed to fully appreciate the complexity of tissue development, the establishment and progression of malignancy, and the transcriptional and trans- lational control of ... See full document

... an ICP0 null virus is hypersensitive in part because it fails to degrade this cellular ...(a cell line that complements ICP0 mutant viruses [36]) cells in the presence and absence of ... See full document

... Herpes simplex virus (HSV) infection in vivo is character- ized by productive infection at peripheral sites followed by viral spread to innervating sensory ...viral gene expression ... See full document

... and cellular proteins that interact with ICP0, we performed affinity purification to isolate ICP0-contain- ing complexes from infected-cell lysates, followed by liquid chro- matography with ... See full document

... of ICP0 to target specific cellular proteins for ubiquitination and proteasome-dependent degradation is thought to be crucial for promoting high levels of viral gene expression, replication, ... See full document

... of ICP0 in the cytoplasm may be caused by the cellular proteasomal machinery (24), and indeed, ICP0 has been shown to induce the accumulation of colocalizing conjugated ubiquitin in both the nucleus ... See full document

... programmed cell death process which is activated during normal develop- ment and by various stimuli, such as viral infection, which disturb cellular metabolism and ...That herpes simplex ... See full document

... In an attempt to determine whether the growth status of cells in the monolayer affects the ability of ICPO mutants to initiate plaque formation, the plating efficiencies and abilities of[r] ... See full document