[PDF] Top 20 Precise Identification of a Human Immunodeficiency Virus Type 1 Antigen Processing Mutant

... Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus ...the processing of the optimal peptide ...of precise rules ... See full document

... (ts) human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) mutant was generated by charged-cluster-to-alanine ...The mutant virus, ... See full document

... site mutant forms (124mut and 134mut) (Table 1) with mutations in the 2G12 epitope were tested for cell surface DC-SIGN (A), soluble DC-SIGN (ECD) (B), and ... See full document

... the mutant tRNA Lys3 in human cells, a retroviral vector system was optimized [34] and ...the mutant tRNA Lys3 s were not significantly affected, indicating the mutants did not interfere with ... See full document

... simian immunodeficiency virus SIVcpz sequences deposited in the Los Alamos National HIV Se- quence Database to date ...or mutant envelope glycoproteins were incubated at different tempera- tures for ... See full document

... HIV type 1 (HIV-1) Tat protein modulated the proteasome composition and activ- ity by upregulating ␤ 2i and ␤ 5i and downregulating ␤ 1i, result- ing in the generation and presentation of subdominant ... See full document

... monitor mutant and wild-type glycoprotein expression following transfection of cells with plasmid DNA ...double mutant is partially defective for gp160 ...binding mutant (24) nor the 41.2 ... See full document

... To identify the cis-regulatory sequences of the HIV-1 LTR target for EBNA2 activation, we performed cell transfection experiments in which the wild-type or mutant HIV-1 LTR plasmids carr[r] ... See full document

... with human immunodeficiency virus type 1 (HIV-1) requires the viral protein ...Progeny virus was produced which was noninfectious in PBL but replicated in SupT1 ...protein ... See full document

... wild type, 604del, and revE1 contained similar levels of unintegrated HIV-1 cDNA ...wild type, 604del, and revE1 were processed by inte- grase in ...wild type (Table 1) differs ... See full document

... double mutant con- taining a start codon deletion and premature termination codon midway through the coding ...Fig. 1 to compare levels of HIV production that would result from stable integration of these ... See full document

... conditional mutant viruses which would be useful for identification of amino acids required for the various functions of integrase during the course of infection and possibly for identification of ... See full document

... deletion mutant 50-288 yielded 10 to 20% of wild-type levels of 3 9 processing and DNA strand transfer activities ...9 processing and DNA strand transfer activities are catalyzed by large ... See full document

... viral antigen (p24) in the stocks was determined by an enzyme immunoassay as recommended by the manufacturer ...a 1:10,000 dilution of pooled patient antisera (a gift of ...for 1 h at 258C with a ... See full document

... and processing of the viral core, is very critical for optimal viral infectivity ...the virus is an essential step at the early stage of infection of target cells ... See full document

... HIV Tat protein influences a large number of viral and host functions. For some of these, the functional regions have been defined. The basic region which contains an arginine-rich re- gion (positions 49 to 57) has been ... See full document

... However, we feel that the variation that we observe in the abundance, or even detectability, of some tRNA-sized species present in HIV-1 produced in different cell types 18 argues agains[r] ... See full document

... 174175IR-AA mutant has lost degradation activity against ...174175IR-AA mutant does not antagonize FcaA3Z2bZ3 antiviral ...174175IR-AA mutant still has the capability to bind to FcaA3s, ELOB, and ... See full document

... AIDS that is amenable to a variety of experimental parameters while having many of the hallmarks of HIV-1 infection and pathogenesis, including the establishment of latently infected populations of cells (7, 12). ... See full document

... Wild-type HIV-1 Gag particles visualized by cEM. We have previously proposed a model for the arrangement of Gag polyproteins in immature HIV-1 particles (13). Here we pro- vide experimental evidence ... See full document