[PDF] Top 20 Processing of rabbit hemorrhagic disease virus polyprotein.

... the processing of construct pRC56P, yielded the sequence Gly-Ala-Asn-Arg-Phe-Asn, which corresponded to residues 719 to 724 of RHDV polypro- ...RHDV polyprotein cleavage products p43 and p73, corre- sponded ... See full document

... protein, p69, was barely detectable with this antiserum, sug- gesting that p69 is not part of segment I. Both proteins extend beyond the C terminus of the protease, which is 15 kDa in size (54), and contain at least part ... See full document

... polymerase such plasmid made RNA transcripts which, after autolytic cleavage, yielded a 2.2-kb RNA transcript with a nu- cleotide sequence identical to that of the authentic subgeno- mic mRNA of RHDV, lacking the VPg ... See full document

... A virus (7). Expression of the hepatitis A virus 2C protein was inhibitory to the growth and protein synthesis of bacteria, but deletion of the 2C N- terminal amphipathic helix (21 amino acid residues) ... See full document

... RHDV2 virus has raised concerns regarding the impact of the new disease among domestic and wild rabbit popula- tions ...MAbs, rabbit immune serum and virus capsid antigen obtained from ... See full document

... MV strain by cell culture passage attenuation of virulent field strains, as these attenuated viruses usually lack the ability to spread through horizontal transmission (47, 54, 55). Presum- ably, these attenuated MV ... See full document

... The calicivirus minor capsid protein VP2 is expressed via reinitiation of protein synthesis after termination of translation of the preceding VP1 gene. A sequence element of about 80 nucleotides denoted “termination ... See full document

... On the other hand, replacement with Ser, which maintains the nucleophilic character of Cys but does not have similar structural functions such as the ability to form disulfide bridges, y[r] ... See full document

... cells were infected with 10 moi of vORF1 for 1 hour, fol- lowing which the virus was replaced with Excel 405 medium. For a time-course, the infected cells were har- vested at 24, 48, 60 and 72 hours post-infection ... See full document

... 10 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Recombinant Rabbit Hemorrhagic Disease Virus Capsid Protein Expressed in Baculovirus Self-Assembles into Viru[r] ... See full document

... as rabbit calicivirus disease (RCD) or viral haemorrhagic disease (VHD), a highly infectious and often fatal disease that affects wild and domestic ...the virus infects only rabbits, ... See full document

... simplex virus 1 (HSV1) or vesicular stomatitis virus (VSV) ...C virus (HCV), Dengue virus, or human immunodeficiency virus (HIV)-1, have evolved mechanisms to evade autophagy and in ... See full document

... Protein sequencing. To determine the N-terminal sequence of protein p35, ASFV proteins from Percoll-purified virions were resolved by 2-D gel electro- phoresis and then transferred to a polyvinylidene difluoride ... See full document

... “Sapporo-like virus”), in which rabbit hemorrhagic disease virus (RHDV), feline calicivirus (FCV), Norwalk virus, and Sapporo virus are assigned as the prototype strains ... See full document

... fever virus (ASFV) is the causative agent of a severe hemorrhagic disease of domestic ...icosahedral virus that infects macro- phages/monocytes of swine as well as ticks of the Ornithodoros ... See full document

... in rabbit samples collected after October 2011 are highly related to the first RHDV2 strains identified in France and Italy [10-12] with an average nucleotide identity at VP60 of ... See full document

... cloned virus and parental JX/ CHA/97 ...cloned virus (tagged with the EcoRV site) and parental virus (lacking an EcoRV ...cloned virus or the parental JX/ CHA/97 isolate (lane A), and a 443-bp ... See full document

... the polyprotein fits with the organiza- tion of all members of the picornavirus superfamily ...foot-and-mouth disease virus, only two processing products of 3AB are known (9, 17); in contrast, ... See full document

... the disease is very difficult because of its epide- miological ...fibroma virus, another lepori- poxvirus, and a cell culture-attenuated strain of MV (SG33) is used for booster injections ...contagious ... See full document

... hunting, rabbit populations continued to grow rapidly, with ma- jor plagues recorded throughout the 20th century; the total rabbit populations in Australia were estimated to be in the hundreds of millions ... See full document