[PDF] Top 20 Proteins Specified by Herpes Simplex Virus

... Complete mixture 199 and a special formulation lacking arginne, leucine, isoleucine, and valine were obtained 123 Downloaded from http://jvi.asm.org/ on November 11, 2019 by guest Membra[r] ... See full document

... We conclude that the virus-specified glycoproteins are present both in contaminating membrane fragments and in the virion since i they are to a small extent separable from the bulk of th[r] ... See full document

... Sephadex G50 gel filtration chromatography of glycopeptides obtained by Pronase digestion of: a a total lysate of uninfected cells labeled with D- ["Clglucosamine from 4 to 12 h after mo[r] ... See full document

... A recent study of the structural polypeptides specified by 53 different HSV-1 strains revealed that certain polypeptide bands with characteristic electrophoretic mobilities in SDS gels w[r] ... See full document

... The estimates of the percentage of total virion protein contributed by each polypeptide are based on computer-aided planimetric analysis both of autoradiograms of polyacrylamide gels con[r] ... See full document

... Staining and Radiolabeling Properties of B Capsid and Virion Proteins in Polyacrylamide Gels WADE GIBSON AND BERNARD ROIZMAN Departments of Microbiology and Biophysics, The University of[r] ... See full document

... Evidence that most of the ICP we have identi0 fied are specified by viral genetic information is -W based on three types of experiments dealing with comparisons of polypeptides synthesiz[r] ... See full document

... Characterization and Composition of Multiple Capsid Forms of Subtypes 1 and 2 WADE GIBSON AND BERNARD ROIZMAN Department of Microbiology, The University of Chicago, Chicago, Illinois 606[r] ... See full document

... The first point addresses itself to the stability of the viral glycoprotein-membrane bond particularly in the course of extensive manipula- Downloaded from http://jvi.asm.org/ on Novembe[r] ... See full document

... of herpes simplex vi- rus 1 were investigated. The capsid of herpes simplex virus is built up of a variety of proteins, and we scrutinized the influence of two of these ... See full document

... Herpes simplex virus 1 (HSV-1) is a double-stranded DNA virus that replicates in the nucleus of the host cell and is known to interact with several components of the cellular ... See full document

... Of the homologues, BICP0 had an effect similar to that of ICP0 (Fig. 5D to F), except that in some cells PML was redis- tributed into strange, globular, sometimes arc-like structures, some of which were coincident with ... See full document

... Autoradiogram of an SDS-acrylamide gel slab showing the electrophoretic separation of [35S]methionine aa-labeled and [14Cjglucosamine g-labeled proteins present in detergent-solubilized [r] ... See full document

... Fluor 546, and PML was conjugated to Alexa Fluor 647. Images were captured using a Zeiss LSM 510 confocal nonlinear optics (NLO) microscope or a Zeiss LSM 780 confocal microscope equipped with argon and HeNe lasers and ... See full document

... herpesvirus proteins and nucleoporins in the process of capsid- nucleus attachment, antibodies specific for selected proteins were introduced directly into the cytoplasm of cells by syringe loading (see ... See full document

... capsid proteins were expressed in recombinant baculoviruses using the BAC-to-BAC system (Invitrogen) ...the proteins encode a six- histidine handle at the N ...etch virus cleavage site between the ... See full document

... There are some lines of evidence that suggest that gH/gL is a fusion protein. The gH/gL complexes of VZV and CMV have been reported to independently execute some level of cell-cell fusion (14, 37). HSV-1 gH/gL has been ... See full document

... 2265 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The specificity of herpes simplex virus type 1-specific cytotoxic T cells was examined with target cells expressing[r] ... See full document

... In conclusion, our studies suggest that following infection of a cell, input (nucleosome-less) HSV DNA, from virions docked at the nuclear pore, is complexed with histones to form nucleosomes within 1 h. These histones ... See full document

... When maximum levels of the ICSP 11,12 protein were attained about 18 h at 37°C postinfection Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The major herpes simplex vi[r] ... See full document