[PDF] Top 20 Proteolytic processing of Ty3 proteins is required for transposition.
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Proteolytic processing of Ty3 proteins is required for transposition.
... A linker insertion mutation near the putative active site in Tyl protease blocks processing of the precursor polyprotein to mature Tyl proteins and results in accumulation of immature pa[r] ... See full document
10
Proteolytic Processing and Assembly ofgag and gag-pol Proteins of TED, a Baculovirus-Associated Retrotransposon of the Gypsy Family
... and Ty3 of Saccharomyces cerevisiae, synthesis of gag-en- coded structural proteins is required for transposition, suggest- ing that VLP assembly is an important intermediate step in this ... See full document
7
Furin Processing and Proteolytic Activation of Semliki Forest Virus
... that proteolytic maturation of the prM protein was mediated by furin and required prior exposure of the virus to mildly acidic pH (21, ...p62 processing normally occurs prior to budding ...furin ... See full document
9
Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.
... 5.4-kbp Ty3 VLP DNA in ...amino-terminal processing, sug- gesting that the proteins were not grossly ...against Ty3 IN and was found only in cells expressing Ty3 (33, ...to Ty3 ... See full document
11
Proteolytic Processing of Turnip Yellow Mosaic Virus Replication Proteins and Functional Impact on Infectivity
... protein processing in TYMV replication, reverse-genetics experiments were per- ...that processing at the HEL/POL junction is absolutely required for TYMV infectivity, as the TYMV HEL/POL mutant was ... See full document
11
Proper Processing of Avian Sarcoma/Leukosis Virus Capsid Proteins Is Required for Infectivity
... structural proteins and enzymes of retroviruses are syn- thesized as part of Gag or Gag-Pol ...envelope proteins and genomic RNA, aggregate at the cell membrane to form and subsequently bud immature virus ... See full document
6
Molecular Characterization of Proteolytic Processing of the Pol Proteins of Human Foamy Virus Reveals Novel Features of the Viral Protease
... Pol proteins have been shown previously to express active HFV reverse transcriptase (RT), RNase H (RH), and integrase (IN), to date, proteolytic processing of HFV Pol proteins has not been ... See full document
5
Site 1 Protease Is Required for Proteolytic Processing of the Glycoproteins of the South American Hemorrhagic Fever Viruses Junin, Machupo, and Guanarito
... membranes. Proteins were stained with Coomassie brilliant blue, membranes were destained extensively, and the bands corresponding to the GP2 parts of the recombinant GPs were ... See full document
7
Ty3 Integrase Is Required for Initiation of Reverse Transcription
... of Ty3, GAG3 and POL3, are analogous to the retroviral gag and pol ...structural proteins capsid (CA) and nucleocapsid (NC) and the enzymes protease (PR), reverse transcriptase (RT), and integrase (IN), ... See full document
13
Regulation of Mitochondrial Dynamics by Proteolytic Processing and Protein Turnover
... receptors proteins: Fis1, mitochondrial fission factor (Mff), mitochondrial dynamics proteins of 49kDa (MiD49) and 51kDa (MiD51) ...receptor proteins plays important roles for ... See full document
19
Mutations in the Ty3 major homology region affect multiple steps in Ty3 retrotransposition.
... be required for proteolytic processing and reverse transcription, or a loss of protease (PR) and re- verse transcriptase (RT) activities caused by incorrect tertiary structure of ...the Ty3 ... See full document
9
Mutations in Nonconserved Domains of Ty3 Integrase Affect Multiple Stages of the Ty3 Life Cycle
... produce Ty3 IN mutants that are defective for transposition but retain RT and IN 39-end processing ...of Ty3 IN was successfully used to create such ...the Ty3 life cycle (Fig. 9). ... See full document
13
Cathepsin L Is Involved in Proteolytic Processing of the Hendra Virus Fusion Protein
... The proteolytic processing of Hendra virus F by an endoso- mal protease and the presence of both uncleaved and cleaved F proteins on the surfaces of Hendra virions suggest that Hen- dra virus can ... See full document
7
A Bipartite Membrane-Binding Signal in the Human Immunodeficiency Virus Type 1 Matrix Protein Is Required for the Proteolytic Processing of Gag Precursors in a Cell Type-Dependent Manner
... Coimmunoprecipitation. The SupT-1-derived T-cell lines were lysed by incu- bation at room temperature for 5 min in phosphate-buffered saline (PBS) con- taining 1% Triton X-100, and the cell lysates were clarified by ... See full document
8
Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumavirus
... absolutely required for infectivity and processing, since the PR D/A mutant, in which the Asp residue of the catalytic center was replaced by Ala, resulted in a noninfectious HSRV provirus ...(12). ... See full document
5
Ty1 Proteolytic Cleavage Sites Are Required for Transposition: All Sites Are Not Created Equal
... is required for cleavage at the other ...is required for activity at the other sites, or (ii) the N-terminal residues which are altered in the Gag*PR mutant might be required for ...is ... See full document
7
Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins.
... In flavivirus, NS3 is necessary, but not sufficient, for processing at the nonstructural cleavage sites: in addition to the catalytic NS3 domain, NS2B is required for proteolytic activit[r] ... See full document
8
Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins.
... Four separate regions of homology were identified within the N-terminal 180 amino acids of NS3; three encompassed the catalytic 2467 Downloaded from http://jvi.asm.org/ on November 10, 2[r] ... See full document
9
Proteolytic processing of imported chloroplast proteins
... foreign proteins, can import into chloroplasts with the appropriate transit peptide (see Keegstra, 1989) but fails to explain why a folded precursor cannot be imported (Della Cioppa and Kirshore, ...is ... See full document
206
The proteolytic processing of organellar proteins
... soluble processing peptidase involved in the maturation o f mitochondrial precursor proteins was extracted from yeast mitochondria as a complex of MW « 115,000 which dissociated into several subunits upon ... See full document
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