[PDF] Top 20 From Quellung to Multiplex PCR, and Back When Needed, in Pneumococcal Serotyping
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From Quellung to Multiplex PCR, and Back When Needed, in Pneumococcal Serotyping
... isolated from cerebrospinal fluid (CSF) or blood are sent to the National Institute for Health and Welfare (THL) for species confirmation and ...serotyping. Serotyping has been performed for several ... See full document
5
Serotyping of Streptococcus pneumoniae Isolates from Nasopharyngeal Samples: Use of an Algorithm Combining Microbiologic, Serologic, and Sequential Multiplex PCR Techniques
... sequential multiplex PCR (MP-PCR) techniques to serotype the ...and Quellung tests (LA/Q) as well as a series of six sequential MP-PCR ...Results from the two methods agreed for ... See full document
6
Rapid and Easy Identification of Capsular Serotypes of Streptococcus pneumoniae by Use of Fragment Analysis by Automated Fluorescence Based Capillary Electrophoresis
... specific pneumococcal serotypes from isolate sets (20, 29) and also from clinical specimens (3, 23, 31, 33, ...44). Multiplex PCR-based methods offer a simple and economical approach ... See full document
7
Accuracy of High Throughput Nanofluidic PCR Based Pneumococcal Serotyping and Quantification Assays Using Sputum Samples for Diagnosing Vaccine Serotype Pneumococcal Pneumonia: Analyses by Composite Diagnostic Standards and Bayesian Latent Class Models
... in pneumococcal vaccine stud- ...nanofluidic PCR-based pneumococcal serotyping and quantification assay methods using sputum samples (the nanofluidic sputum quantitative PCR [Sp-qPCR] ... See full document
10
Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples
... quantified PCR products, the detection limit was estimated to be ...of pneumococcal isolates of the 45 serotypes most prevalent among IPD patients in The Netherlands were ...no PCR products in 1 of ... See full document
10
Revisiting Pneumococcal Carriage by Use of Broth Enrichment and PCR Techniques for Enhanced Detection of Carriage and Serotypes
... to PCR-based serotype deduction that must still be ...yielding PCR-based serotyping results also yielded a corresponding control band for the cpsA gene target, the sequence of which is highly con- ... See full document
8
Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... 23 pneumococcal “vaccine” serotypes is clearly less discriminatory than conventional serotyping (1–3, 6; Kong et ...the Quellung reaction; ... See full document
5
Serotyping of Streptococcus pneumoniae isolated from Tehran by Multiplex PCR: Are serotypes of clinical and carrier isolates identical?
... Previous studies indicate that young children in Iran experience a high prevalence of S. pneumoniae carriage rate. A study of 1300 healthy children less than ten years-old in Tehran, Iran showed pneumococcal ... See full document
7
Sequential multiplex PCR assay for determining capsular serotypes of colonizing S pneumoniae
... for PCR-based serotyping developments ...plex PCR assay specific to invasive pneumococcal dis- eases (IPD) distribution in the USA was developed by Pai and colleagues ...detection from ... See full document
9
Evaluation of a Multiplex PCR Test for Simultaneous Identification and Serotyping of Actinobacillus pleuropneumoniae Serotypes 2, 5, and 6
... Various PCR tests have been developed for species identification of ...these PCR tests is that the primers used for amplification are intended to be specific for ...still needed for ...DNA ... See full document
6
Serotyping Streptococcus pneumoniae by Multiplex PCR
... (ii) Multiplex reaction ...of multiplex reactions were developed, taking into account the size of the PCR products and the compatibility between primers (the optimized primer conditions are ... See full document
7
Concurrent Serotyping and Genotyping of Pneumococci by Use of PCR and Electrospray Ionization Mass Spectrometry
... A pneumococcal serotyping/genotyping system (PSGS) was developed based upon targeted PCR, followed by electrospray ion- ization mass spectrometry and amplicon base composition ...Eight ... See full document
8
Challenges of Using Molecular Serotyping for Surveillance of Pneumococcal Disease
... of pneumococcal DNA (lytA) by a PCR or a positive latex antigen test with a matched Gram stain result from a normally sterile site specimen ...subcultured from Dorset transport medium onto 5% ... See full document
6
Development of a Multiplex PCR Assay Targeting O Antigen Modification Genes for Molecular Serotyping of Shigella flexneri
... were from Li et ...solution. PCR amplification and detection. Multiplex PCR were performed using Qia- gen multiplex PCR kit ...each PCR consisted of 1 ⫻ PCR Master ... See full document
5
Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... tiplex PCR differs depending on the template used. When RNA prepared from viruses grown in cell culture or from clinical samples was used as a template, the SYBR green RT- PCR displayed ... See full document
7
Isolation, Biochemical Characterization, PCR, Serotyping and Antibiogram of Salmonella from Chickens
... samples from 160 birds each Liver, Heart, Spleen and Gall Bladder, 40 eggs, 10 ovary samples, 25 faecal and feed samples from poultry farms in and around Bangalore were screened for Salmonella serovars ... See full document
5
Detection of and Discrimination between Gram Positive and Gram Negative Bacteria in Intraocular Samples by Using Nested PCR
... isolated from ocular infections include ...undertaken when sufficient sample is available for the full array of culture media to be ...inoculated. PCR techniques, on the other hand, only require very ... See full document
5
Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes
... The PCR started with an enzyme activation step at 94°C for 15 min, then 40 cycles at 94°C for 1 min, 50°C for 1 min and 72°C for 1 min, followed by a final extension step at 72°C for 7 ...QIAquick PCR ... See full document
7
Optimization and validation of multi coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)
... steps from DNA extraction to CE; (ii) multiplex amplification of the outer msp1 and msp2 blocks in the primary reaction; (iii) multiplexing the CE runs with three and two colours for msp1 and msp2, ... See full document
14
Detection and Serotyping of Dengue Virus in Serum Samples by Multiplex Reverse Transcriptase PCR Ligase Detection Reaction Assay
... the multiplex capability of the PCR-LDR assay. A multiplex PCR amplification of both target regions used all 46 PCR primers in a single ...a multiplex ligation reaction using the ... See full document
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