[PDF] Top 20 Sensitive reverse transcriptase assay to detect and quantitate human immunodeficiency virus
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Sensitive reverse transcriptase assay to detect and quantitate human immunodeficiency virus
... A sensitive biochemical assay of viral reverse transcriptase RT was developed that is useful for both the detection and quantitation of human immunodeficiency virus HIV, the agent respon[r] ... See full document
5
Effects of Amino Acid Substitutions at Position 115 on the Fidelity of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... Previous reports on the fidelity of Y115 mutants were based either on a biochemical measure of misinsertion (Y115F) (18) or on mispair extension (Y115F and Y115V) (19). Both Y115F and Y115V were reported to be more error ... See full document
7
Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase.
... HIV-1 reverse transcripts was not included in these analyses, as they are abundant intravirion HIV-1 reverse tran- scripts ...junction assay (26) (data not ... See full document
16
Novel Recombinant Virus Assay for Measuring Susceptibility of Human Immunodeficiency Virus Type 1 Group M Subtypes To Clinically Approved Drugs
... suppress human immunodeficiency virus (HIV) replication in pa- tients but selects for drug resistance, requiring subsequent resistance-guided therapeutic ...novel assay that offers a uniform ... See full document
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Characteristics and clinical significance of a stabilization assay to detect specific antibodies to reverse transcriptase of human immunodeficiency virus.
... to detect antibody against Pol proteins (p66/p51 or p64/p53) (6), and the intensities of p66 and p51 protein bands correlated with the stabilizing activities in sera ...neutralization assay or stabilization ... See full document
7
Genotypic, Phenotypic, and Modeling Studies of a Deletion in the β3-β4 Region of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Gene That Is Associated with Resistance to Nucleoside Reverse Transcriptase Inhibitors
... Point mutations and inserts in the  3-  4 region of human immunodeficiency virus type 1 (HIV-1) reverse tran- scriptase (RT) are associated with resistance to nucleoside analog inhibitors. ... See full document
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Comparison of Second-Strand Transfer Requirements and RNase H Cleavages Catalyzed by Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) and E478Q RT
... transfer assay with substrates possessing truncations in the 5⬘ RNA ...polymerase-dependent assay, the RNase H active site would be positioned within a double-stranded DNA ...This assay therefore ... See full document
12
The Impact of Multidideoxynucleoside Resistance-Conferring Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase on Polymerase Fidelity and Error Specificity
... Human immunodeficiency virus type 1 (HIV-1) infections are characterized by a high degree of genetic variation, which inevitably leads to facile generation of drug-resistant variants during treatment ... See full document
6
Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract.
... Although both zinc fingers are required for HIV-1 infectivity (22, 32, 34), the molecular details of RNA binding and the role of the two zinc fingers are not understood. As a result, it is difficult to interpret the ... See full document
11
Sensitive Phenotypic Detection of Minor Drug Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Variants
... of assay does not monitor the phenotype of individual virus genomes, and the phenotype of minor variants in the viral population may be ...assays detect drug resistance only when resistant variants ... See full document
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Sources and Magnitude of Intralaboratory Variability in a Sequence Based Genotypic Assay for Human Immunodeficiency Virus Type 1 Drug Resistance
... to detect minor variants either by genotyping or by phenotyping methods should be a recognized limitation of clinical antiretroviral drug resistance testing (4, ...the reverse transcription step to sequence ... See full document
8
High Sequence Conservation of Human Immunodeficiency Virus Type 1 Reverse Transcriptase under Drug Pressure despite the Continuous Appearance of Mutations
... To detect the overall selection pressure at the amino acid level of HIV-1 RT, nucleic acid sequences of 179 randomly selected patients with clade B pol subtype were ... See full document
12
Increased Multinucleoside Drug Resistance and Decreased Replicative Capacity of a Human Immunodeficiency Virus Type 1 Variant with an 8-Amino-Acid Insert in the Reverse Transcriptase
... Resistance to antiretroviral drugs is generally conferred by specific amino acid substitutions, rather than insertions or deletions, in reverse transcriptase (RT) of human immunodeficiency ... See full document
8
Reduced Susceptibility of Human Immunodeficiency Virus Type 1 (HIV-1) from Patients with Primary HIV Infection to Nonnucleoside Reverse Transcriptase Inhibitors Is Associated with Variation at Novel Amino Acid Sites
... The reduced susceptibility described was not associated with mutations at amino acid sites that were previously known to affect susceptibility to NNRTIs, and its genotypic basis re- mained unclear. We therefore extended ... See full document
5
The K65R Mutation in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Exhibits Bidirectional Phenotypic Antagonism with Thymidine Analog Mutations
... agent Program, National Institute of Allergy and Infectious Diseases, National Institutes of Health). Seven days after transfection, cell-free supernatant was harvested and stored at ⫺80°C. The genotype of stock viruses ... See full document
7
Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... The identification and characterization of RT mutants has proven to be invaluable for integrating structural and func- tional properties of this critical HIV-1 protein. Both in vitro mutagenesis of the HIV-1 RT and ... See full document
8
Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Can Prevent Self-Priming of Minus-Strand Strong Stop DNA by Promoting the Annealing of Short Oligonucleotides to Hairpin Sequences
... Complete reverse transcription of PBS-R RNA starting from a 19-base DNA primer annealed at the PBS produced a 200-base-long DNA ...representative reverse transcription experiment is shown in ... See full document
8
Differentiation between human immunodeficiency virus type 1 (HIV 1) and HIV 2 isolates by nonradioisotopic reverse transcriptase typing assay
... We tested whether human immunodeficiency virus type 1 HIV-1 could be differentiated from HIV-2 by a reverse transcriptase RT-typing assay that measured the reduction of enzyme activity o[r] ... See full document
6
Amino Acid Mutation N348I in the Connection Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Confers Multiclass Resistance to Nucleoside and Nonnucleoside Reverse Transcriptase Inhibitors
... production assay and a compet- itive HIV-1 replication assay ...production assay, acquisition of N348I drastically impaired replication in MT-2 and SupT1 cells ... See full document
10
Role of etravirine in the management of treatment-experienced patients with human immunodeficiency virus type 1
... second-generation human immunodeficiency virus type 1 (HIV-1) non-nucleoside reverse transcriptase inhibitor (NNRTI) with expanded antiviral activity against NNRTI-resistant HIV-1, to ... See full document
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