[PDF] Top 20 Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... the assembly process was confirmed by the results obtained with the 48-bp site IIm ⫹ EP oligonucle- otide ...3); T-ag assembly on this molecule was limited to hexamer formation ...both ... See full document
13
Phosphorylation of Simian Virus 40 T Antigen on Thr 124 Selectively Promotes Double-Hexamer Formation on Subfragments of the Viral Core Origin
... SV40 replication in vivo. Upon syn- thesis, T-ag is rapidly transported as monomers from the cy- toplasm to the nucleus ...SV40 core origin at all stages of the cell ...SV40 replication ... See full document
13
Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA Unwinding
... duplex origin DNA and viral DNA replication (4–6, 14, ...the origin (the present study and reference ...in origin unwinding, a truncated T antigen lacking the first 123 ... See full document
11
Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly
... full-length T-ag and the site I ⫹ wt 30 oligonucleotide is presented in lane ...purified T-ag OBD is presented in lane ...Full-length T-ag forms single hexamers on site I-containing ...SV40 ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) shows ... See full document
11
Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... the T-antigen OBD would in- crease substantially its affinity for DNA, as we have observed for the related DBD of the papillomavirus E1 helicase ...the T- antigen OBD would indeed increase its ... See full document
7
Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... with T antigen and pol-prim to co- ordinate the assembly of the replication forks (reviewed in references 26 and ...of T antigen in mediating priming by pol-prim during ... See full document
10
Model for T-Antigen-Dependent Melting of the Simian Virus 40 Core Origin Based on Studies of the Interaction of the Beta-Hairpin with DNA
... SV40 origin that occur during T-ag ...for T-ag oligo- merization on substrates containing a duplex EP region but not for oligomerization on substrates containing a single-stranded 3 ⬘ extension of ... See full document
11
Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD which are defective for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates (i.e., the core origin [0], site I ... See full document
12
Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... Viral replication of single-point ...DNA binding activity of T antigen, we generated mutants with single-point substitu- tions within the region identified ...weak sequence simi- ... See full document
9
Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...The replication of simian virus 40 (SV40) DNA has ... See full document
14
Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... SV40 core origin and structure of SV40 large T-antigen origin binding ...DNA sequence of the SV40 core ...SV40 core origin is depicted as B-form DNA, ... See full document
9
The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...DNA replication, T antigen must ... See full document
7
The Simian Virus 40 Core Origin Contains Two Separate Sequence Modules That Support T-Antigen Double-Hexamer Assembly
... SV40 core origin support the assembly of T-ag double hexamers (this study and references 32, 33, and 35), the entire core origin region is needed for initiation of DNA unwinding ... See full document
12
Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin.
... We have also used DNA footprinting techniques to show indirectly that the assembly of T antigen leads to stepwise and cooperative binding to the early and late halves of the SV40 core or[r] ... See full document
9
The T-antigen-binding domain of the simian virus 40 core origin of replication.
... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document
7
Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
... However, when chromatin was isolated from the variant 1103 19, which contains nine copies of the origin of DNA replication and lacks at least one and possibly two of the other high-affin[r] ... See full document
10
Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... small t is able to mediate, in cooperation with large T, metabolic stabi- lization of p53 in complex with large ...Small t is a transacti- vator of polymerase II and III promoters (5, 35, 45, 46), it ... See full document
9
Simian virus 40 large T antigen host range domain functions in virion assembly.
... When virus-infected cell extracts are separated on 5 to 40%0 sucrose gradients, wild-type extracts show the greatest accumulation of viral late protein in the 200S-240S fractions corresp[r] ... See full document
14
Simian virus 40 small-t antigen stimulates viral DNA replication in permissive monkey cells.
... To explore this apparent paradox between in vivo and in vitro experiments, we examined the effect of small t on replication of microinjected viral DNA and the effects of small-t antigen [r] ... See full document
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