[PDF] Top 20 Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides
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Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides
... of sequence-specific binding of NC to single- stranded oligodeoxynucleotides; partial analysis of sequence ...as binding to single-stranded nucleic acids at a ratio of one NC molecule ... See full document
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Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Can Prevent Self-Priming of Minus-Strand Strong Stop DNA by Promoting the Annealing of Short Oligonucleotides to Hairpin Sequences
... DNA binding protein (40), but we found that this was not the ...the oligonucleotides relative to the hairpin itself; this could reduce the kinetic ad- vantage of hairpin formation (25, ...NC ... See full document
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Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein
... further binding studies with in vitro-translated pro- teins. Binding of a carboxy-terminal p300 region (amino acids 1542 to 2412) was readily detected ...detectable binding to GST-Tat was observed ... See full document
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Structural Consequences of Cyclophilin A Binding on Maturational Refolding in Human Immunodeficiency Virus Type 1 Capsid Protein
... CA protein in precleared samples was immunoprecipitated by ad- dition of CA-specific rabbit ...to protein A-beads by adding fresh slurry to the mixture and incubating for another hour at 5°C, this ... See full document
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Role of the N-Terminal Zinc Finger of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein in Virus Structure and Replication
... FIG. 3. Analysis of early and late steps of viral DNA synthesis by PCR. Infection of HeLa P4 cells was performed with wt and mutant viruses adjusted to the same level of viral RNA. Viral DNA synthesis was analyzed by PCR ... See full document
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The human foamy virus Bel-1 transcription factor is a sequence-specific DNA binding protein.
... evident sequence homology either to each other or to the Int promoter element, and it therefore remains unclear how a DNA target for Bel-1 is defined at the primary sequence ...a ... See full document
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Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging
... structs. Virus produced from construct pHX/MT-NC packaged approximately half as much unspliced viral RNA as did the wild type, and it packaged spliced RNA at levels equivalent to unspliced RNA ...NC ... See full document
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Specific binding of human immunodeficiency virus type 1 (HIV-1) Gag-derived proteins to a 5' HIV-1 genomic RNA sequence.
... represent binding to un- specific RNA or protein-protein complexes of various ...EPHIV 1 RNA (lane 4), a strong and discrete new band ...EPHIV 1 RNA, RNase was added in a control ... See full document
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Sequence Homology Required by Human Immunodeficiency Virus Type 1 To Escape from Short Interfering RNAs
... RNA virus infection, can induce the specific degradation of homologous mRNA species, a process termed RNA interference (RNAi) ...termed short interfering RNAs (siRNAs) (3, 21, ...that human ... See full document
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Context-Dependent Phenotype of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation
... Retroviral nucleocapsid (NC) carries out important func- tions throughout the entire retroviral life cycle (2, ...the virus-encoded protease, NC coats viral genomic RNA and subsequently influences early ... See full document
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Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression
... zinc binding (17, 18; Musier-Forsyth et ...acid binding (9, 23). Our results suggest that mutations that change the type of finger rather than preventing the binding of zinc (17, 18; Musier- ... See full document
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Transactivation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by the human foamy virus bel1 protein requires a specific DNA sequence.
... 3236-3240 0022-538X/92t053236-05$02.00/0 Copyright ©D 1992, American Society for Microbiology Transactivation of Human Immunodeficiency Virus Type 1 Long Terminal Repeat-Directed Gene Ex[r] ... See full document
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Identification of a gp41 Core-Binding Molecule with Homologous Sequence of Human TNNI3K-Like Protein as a Novel Human Immunodeficiency Virus Type 1 Entry Inhibitor
... the binding activity of P20 with the NHR and CHR peptides and the 6-HB core formed by the NHR and CHR peptides in different ...weak binding activity with gp41 NHR and CHR peptides ... See full document
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Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.
... The individual proximal cysteine-histidine box mutations were exchanged for the wild-type sequences in the Pr55gag expression construct to determine whether binding to HIV-1 RNA in vitro[r] ... See full document
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The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivity.
... NC protein in the viral context indicate that deletions and amino acid substitutions in the NC coding sequence result in the production of poorly infectious or noninfectious viruses (4, 21, 26–28, ...kemia ... See full document
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Mutations in Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Zinc Fingers Cause Premature Reverse Transcription
... every virus particle contains minus-strand strong-stop DNA, which is a 1,000-fold increase over the amount observed in wild-type virus ...gag sequence present only in unspliced RNA (65), but ... See full document
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Human Immunodeficiency Virus Type 1 Nucleocapsid p1 Confers ESCRT Pathway Dependence
... Tsg101 binding site in p6 became crucial for efficient particle production, and CHMP3-RFP had a profound inhibitory effect on particle ...zipper sequence conferred both L domain dependence and ESCRT pathway ... See full document
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Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro.
... both oligonucleotides, the underlined bases correspond to the substitution of the GT terminal sequence of HIV-1 DNA introduced in the sequence to produce an NdeI restriction ... See full document
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Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly
... by protein multimerizing ...by protein-protein interactions, we constructed MZip, in which a modified GCN4 leucine zipper sequence was inserted into the NC deletion in ...zipper ... See full document
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Extensive sequence-specific information throughout the CAR/RRE, the target sequence of the human immunodeficiency virus type 1 Rev protein.
... 2 0022-538X/92/021139-13$02.00/0 Copyright C 1992, American Society for Microbiology Extensive Sequence-Specific Information throughout the CAR/RRE, the Target Sequence of the Human Immu[r] ... See full document
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