[PDF] Top 20 Simian virus 40 DNA replication: characterization of gaps in the termination region.

... If the gaps in the pulse-labeled pDNA II are localized in a specific region, then cleavage of the newly synthesized strands by RI endonuclease should yield two populations of labeled fra[r] ... See full document

... of replication and the T-ag gene are necessary for transcription at ...any DNA virus sequences except the SV40 poly(A) signal ...promoter region and capsid proteins (KpnB fragment) were ... See full document

... SV40 DNA replication, in a reaction containing purified proteins, to bind to ssDNA, to interact physically and functionally with pol-prim and RPA, and to mediate priming and elongation on RPA-coated ...SV40 ... See full document

... However, we demonstrate that mouse DNA sequences present in the SV40 late region of the rescued clone and derived from sequences flanking the SV40 integration site in VLM DNA suppress re[r] ... See full document

... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...the region between residues 501 and 550 is not ...support simian virus 40 (SV40) ... See full document

... helicase activity, to an inability to properly recognize the origin and form a double hexameric structure, or to an activity spe- cifically required for origin unwinding. To investigate the first possibility, we carried ... See full document

... In this study we have characterized the transcriptional intermediates of the SV40 gene A mutant, tsA 58, and measured the RNA polymerase activity contained in tsA58 transcriptional inter[r] ... See full document

... Indeed, SV40 RI molecules obtained from unirradiated infected cells present such gaps close to replication forks when they are analyzed by electron microscopy Fig.. Using the standard sp[r] ... See full document

... The presence of the SV40 control region, including the origin, enhancer, and promoter sequences, was sufficient to cause genomic instability at the site of integration of pSV2neo in five[r] ... See full document

... Although a significant amount of the DNA sedimented in the 21S region of a neutral sucrose gradient, alkaline sucrose gradient analyses indicated that little or no mature component I SV4[r] ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... The large tumor antigen T antigen of simian virus 40 SV40 is a multifunctional regulatory protein that is required for a number of steps in productive viral infection, including DNA repl[r] ... See full document

... The alteration in reentry kinetics was not because the DNAs were smaller; other deletion mutations that were from 76 to 85% of the length of wild-type DNA dl-BE and dl-1133 with a deleti[r] ... See full document

... Footprint and fragment assays led to the following conclusions: i in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A prote[r] ... See full document

... The studies described above indicated that the semipermissive nature of infection of early passage human fibroblasts with SV40 is consistent with a readily detectable level of viral DNA [r] ... See full document

... Alkaline sucrose gradient analysis of replicating molecules in the Hirt extract and in the hypotonic nuclear extract.. Parallel, infected cultures were labeled for 3 min with [3H]thymidi[r] ... See full document

... When the level of polymerase activity and the ratio of early to late strand transcripts synthesized in vitro are compared with levels and types of RNA that are found in vivo, the two agr[r] ... See full document

... Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location 10, 11, 12, 14, the bound protein can be localized at 0.7 i 0.05 on the SV40 D[r] ... See full document

... Influence of the various periods of the cell cycle on adsorption of SV40 Period of the cell cycle Input virus adsorbed per 3 x 10' cells 12.0 Beginning of S 12.0 7.5 S 10.5 11.0 G2 6.8 6[r] ... See full document

... Sucrose gradi- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest I.l ent-purified SV40 NPCs, labeled with [35S]methionine and [3H]thymidine, were treated with increasing [r] ... See full document