[PDF] Top 20 Simian virus 40 DNA replication in nuclear monolayers.

... Although a significant amount of the DNA sedimented in the 21S region of a neutral sucrose gradient, alkaline sucrose gradient analyses indicated that little or no mature component I SV4[r] ... See full document

... AcMNPV, DNA replication in simian virus 40 (SV40) has been extensively ...The virus possesses just one origin and encodes only a single protein involved in the replication ... See full document

... Since binding of T antigen to the p68 subunit was not de- tected in the far-Western assay shown in Fig. 5A, it was tested by immunoprecipitation. Equal amounts of WT and mutant T antigens immobilized on an immunoaffinity ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... The large tumor antigen T antigen of simian virus 40 SV40 is a multifunctional regulatory protein that is required for a number of steps in productive viral infection, including DNA repl[r] ... See full document

... The alteration in reentry kinetics was not because the DNAs were smaller; other deletion mutations that were from 76 to 85% of the length of wild-type DNA dl-BE and dl-1133 with a deleti[r] ... See full document

... Footprint and fragment assays led to the following conclusions: i in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A prote[r] ... See full document

... The studies described above indicated that the semipermissive nature of infection of early passage human fibroblasts with SV40 is consistent with a readily detectable level of viral DNA [r] ... See full document

... Alkaline sucrose gradient analysis of replicating molecules in the Hirt extract and in the hypotonic nuclear extract.. Parallel, infected cultures were labeled for 3 min with [3H]thymidi[r] ... See full document

... When the level of polymerase activity and the ratio of early to late strand transcripts synthesized in vitro are compared with levels and types of RNA that are found in vivo, the two agr[r] ... See full document

... Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location 10, 11, 12, 14, the bound protein can be localized at 0.7 i 0.05 on the SV40 D[r] ... See full document

... Influence of the various periods of the cell cycle on adsorption of SV40 Period of the cell cycle Input virus adsorbed per 3 x 10' cells 12.0 Beginning of S 12.0 7.5 S 10.5 11.0 G2 6.8 6[r] ... See full document

... However, we demonstrate that mouse DNA sequences present in the SV40 late region of the rescued clone and derived from sequences flanking the SV40 integration site in VLM DNA suppress re[r] ... See full document

... Ongoing viral DNA replication panels d and e led to a continuous increase in nuclear matrix-associated SV40 minichromosomes, but a significantly higher proportion of SV40 minichromosomes[r] ... See full document

... Replicating deoxyribonucleic acid DNA definitive analysis of the origin and direction of molecules of simian virus 40 SV40 are small, replication could not be made.. In the present circu[r] ... See full document

... SV40 replication centers, are measures of viral amplification ...SV40 replication centers observed at early and late times postinfec- ...SV40 replication centers and explains the fact that abrogation ... See full document

... To explore this apparent paradox between in vivo and in vitro experiments, we examined the effect of small t on replication of microinjected viral DNA and the effects of small-t antigen [r] ... See full document

... 886 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 SV40 DNA replication[r] ... See full document

... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document

... specific DNA sequence in SV40 was ...T-ag DNA binding sites flanking the origin did not have this ...wild-type virus at 3 days after transfection ...this replication contributes to the easier ... See full document