[PDF] Top 20 Simian virus 40 origin DNA-binding domain on large T antigen.

... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document

... drome domain for DNA unwinding, we generated mutant 31-bp DNAs with a single base-pair substitution at each site within the central ...31-bp origin DNA unwinding and binding assays of ... See full document

... Finally, complexes consisting of SV40 DNA and T-antigen fragments were precipitated in the DNA-binding assay with monoclonal antibodies that recognize the central region of the protein; [r] ... See full document

... for DNA tumor virus studies ...small t antigen, respectively), or dlA2433, a mutant with a misfolded carboxyl half due to a deletion of aa 587 to 589 ...carboxyl-region T-antigen ... See full document

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document

... These activities include origin-specific and nonspecific DNA binding, melting of the imperfect palindromic sequence, untwisting of the AT-rich region, unwinding of origincontaining DNA, [r] ... See full document

... the T-ag OBD with the SV40 origin remain to be ...through T-ag double hexamers assembled on the core ...a T-ag OBD hexamer and its interactions with ...red. T-ag OBD residue Glu260 is ... See full document

... 2901 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The sequence components that direct high-affinity binding of simian virus 40 SV40 T antigen to SV40 origin region I[r] ... See full document

... Fusion proteins of large T antigen lacking the first 200 N-terminal amino acids bound specifically to polyomavirus origin DNA; however, deletions beyond this site resulted in unstable pr[r] ... See full document

... 888 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Proteolytic fragments of simian virus 40 tumor T antigen and T antigen that was dephosphorylated with alkaline phosp[r] ... See full document

... Specific binding to the unmethylated 1,229-base-pair fragment was not observed, even at higher T-antigen concentrations, nor was there binding to the smaller 222-base-pair plasmid fragme[r] ... See full document

... i They clearly show the relationship between different subclasses, i.e., the DNAbinding, monomeric nuclear high-salt fractions on one hand and the nonbinding, oligomeric fractions on the[r] ... See full document

... small t helper function in SV40 transforma- tion and metabolic stabilization of p53 in complex with large ...with large T actively contributes to phenotypic trans- formation by SV40, both ... See full document

... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document

... By testing the effects of single-site mutations on virus replication and by performing a number of DNA-binding assays on replication-negative T-antigen mutants, we have identified severa[r] ... See full document

... Second, wild-type T antigen's nonspecific DNA binding, but not its origin binding, was significantly inhibited with a number of monoclonal antibodies PAb1O4, PAb114, PAb252, PAb4O2, PAb4[r] ... See full document

... Simian virus 40 (SV40) large T antigen (LT) is a multifunctional protein that is important for viral replication and oncogenic ...of DNA damage response signaling, which ... See full document

... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ... See full document

... When virus-infected cell extracts are separated on 5 to 40%0 sucrose gradients, wild-type extracts show the greatest accumulation of viral late protein in the 200S-240S fractions corresp[r] ... See full document

... Nine monoclonal antibodies reacted with determinants located in region I, because the antibodies precipitated small t as well as large T antigens from extracts of SV40-transformed or -in[r] ... See full document