[PDF] Top 20 Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
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Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... with simultaneous infec- tions with different genotypes are more frequently associated with higher HBV DNA levels (4, 5, 12, 13, 26, ...HBV genotypes was found in ...of genotypes in the ... See full document
8
Performance of Version 2 0 of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... and quantification of hepatitis B virus (HBV) DNA are essential for the diagnosis and treatment of chronic HBV ...of real-time PCR assays for HBV DNA quantification ... See full document
7
Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... DNA quantification assay shows its excellent ...CAP/CTM assay was also precise and reproducible, as previously reported (2, 5, ...CTM assay was not specifically validated here, the fact that ... See full document
8
COBAS AmpliPrep COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real Time PCR Assay for Quantification of HBV DNA in Plasma
... a real- time PCR assay based on TaqMan technology—has recently been developed (13, ...other real-time PCR assays for HBV DNA are the incor- poration of an internal ... See full document
7
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... DNA quantification around the world and the Daan kit, which is used widely in China for ...DNA quantification. At the same time, it also dem- onstrated the necessity of using two pairs of primers and ... See full document
9
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... DNA quantification, including hybrid capture and branched DNA (bDNA) signal amplification (19–21, 35), assays based on real- time PCR technology (12) offer the greatest sensitivity and broad- ... See full document
9
Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... neous quantification, performed with the set 1 amplicon amplification, which targets conserved genomic ...for simultaneous HBV quanti- fication and genotyping was ...of genotypes B and ... See full document
7
A Genotype Independent Real Time PCR Assay for Quantification of Hepatitis B Virus DNA
... Patients and serum samples. A total of 169 serum samples collected from patients with chronic HBV infection seen in the Liver Clinics at the University of Michigan Hospital were studied. These patients were participants ... See full document
6
Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
... of hepatitis B virus (HBV) DNA in serum by molecular methods has become the standard for assessment of the replicative activity of ...and quantification of HBV DNA have been ...on PCR, ... See full document
5
Expertise of Laboratories in Viral Load Quantification, Genotyping, and Precore Mutant Determination for Hepatitis B Virus in a Multicenter Study
... HBV genotypes, the data analysis revealed a few discrepancies, essentially due to poor amplification sensitivities of the samples with the lowest VLs and sometimes due to ...G genotypes in the amplified ... See full document
8
Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real Time PCR
... RT-PCR assay was evaluated by testing the method against viral RNAs de- rived from various ...influenza virus [A/New Caledonia/20/99 (H1N1), A/Sydney/5/97(H3N2), and ...coxsackie virus A9 and ... See full document
6
Quantitation of Hepatitis B Virus Genomic DNA by Real Time Detection PCR
... serum virus levels frequently induce fluctuation of serum ALT ...serum virus level is useful in monitoring the clinical course of patients with active hepatitis ...quantitative assay should be ... See full document
5
Application of real time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia
... Hepatitis B virus (HBV) is the leading cause of viral hepa- titis in humans ...the virus, 60 mil- lion of them residing in Africa ...of virus in the ...of hepatitis B ... See full document
7
Rapid and Specific Genotyping System for Hepatitis B Virus Corresponding to Six Major Genotypes by PCR Using Type Specific Primers
... by PCR, the results were in complete accord with the sequences corresponding to their type-specific ...of PCR, type A HBV DNA was amplified with the type A-specific primer, but not with other type-specific ... See full document
5
Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano gold immunological amplification and silver staining method
... The hepatitis B virus (HBV) and hepatitis C virus (HCV) often cause persistent infection, leading to chronic liver diseases, cirrhosis and hepatocellular carcinoma ...as ... See full document
8
Comparison of Conventional PCR with Real Time PCR and Branched DNA Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5
... The quantification standard represents a noninfectious RNA construct containing fragments of HCV sequences with primer binding regions identical to those of the HCV 5⬘ nontranslated region, leading to an ... See full document
9
One Step, Multiplex, Real Time PCR Assay with Molecular Beacon Probes for Simultaneous Detection, Differentiation, and Quantification of Human T Cell Leukemia Virus Types 1, 2, and 3
... tube) real-time PCR method for the simultaneous detection, differ- entiation, and quantification of HTLV-1, -2, and -3 proviral ...saving time. Like other real- ... See full document
7
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... The vectors were linearized using PstI restriction enzyme (Invitrogen, Carls- bad, CA) and run on a 1% agarose gel for confirmation and purification. Bands of the correct size were excised and cleaned using a QIAquick ... See full document
8
The New Aptima HBV Quant Real Time TMA Assay Accurately Quantifies Hepatitis B Virus DNA from Genotypes A to F
... genotype B, 8 with genotype C, 52 with genotype D, 38 with genotype E, and 2 with genotype ...genotype B, 1 with genotype D, and 4 with genotype ...genotype B, 2 with genotype C, 9 with ... See full document
9
Hepatitis C virus replicative levels and efficiency of genotyping by specific PCR and antibody assay
... of hepatitis C virus genotypes by the group-specific PCR of the sequence within the core region gene and by the newly developed genotype-specific NS4 antibody ...assay. ... See full document
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