[PDF] Top 20 Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... The T-Ag OBD is connected to the helicase domain by a flexible linker, and hence much of the surface of the T-Ag OBD hexamer remains solvent accessible even in the context of full-length ...the ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, ... See full document
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Model for T-Antigen-Dependent Melting of the Simian Virus 40 Core Origin Based on Studies of the Interaction of the Beta-Hairpin with DNA
... SV40 origin that occur during T-ag ...for T-ag oligo- merization on substrates containing a duplex EP region but not for oligomerization on substrates containing a single-stranded 3 ⬘ ... See full document
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Simian Virus 40 DNA Replication Is Dependent on an Interaction between Topoisomerase I and the C-Terminal End of T Antigen
... of simian virus 40 (SV40) DNA replication and for the formation of completed DNA ...distinct binding sites for topo I have been previously mapped to the N-terminal (residues 83 ... See full document
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... footprinting, single-stranded oligonucleotides were labeled at their 59 termini by standard methods ...kinase-labeled, single-stranded fragments were hybridized with their comple- mentary ... See full document
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... these binding events. Consistent with this hypothesis, it was reported that T-ag residues 121 to 135 are required for interactions with the AT-rich region while a second, poorly defined region is necessary ... See full document
13
Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... Eukaryotic DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance ... See full document
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Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly
... of T-ag, shown in yellow, binds to site I (the J-domain is not ...the binding of the initial monomer, additional T-ag molecules are recruited to site I owing to the high affinity of the ... See full document
12
Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... T antigen belongs to helicase superfamily 3, which includes helicases from small DNA and RNA viruses ...(22). Based on sequence alignments and secondary structure predictions, Masterson ... See full document
9
Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... open-ring structure (a spiral), and the authors suggest the open ring may provide a mechanism whereby the single-stranded nucleic acid may enter the central channel of the ring without disrupting the ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD spiral hexamer is con- figured such that many of the A1 and B2 residues that interact preferentially with ssDNA in the NMR studies ...disrupt T-ag’s helicase (green) and unwinding (orange) ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... Small DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...large ... See full document
7
Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... viral DNA replication, we examined the relationship between T-antigen oligomeric state and DNA helicase ...of T antigen were incubated with a model DNA replication ... See full document
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Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
... We previously reported 28 that only a very small fraction of T Ag binds with a higher salt-sensitive affinity to SV40 DNA than to calf thymus DNA-cellulose under these conditions and tha[r] ... See full document
5
Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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Discrete regions of simian virus 40 large T antigen are required for nonspecific and viral origin-specific DNA binding.
... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document
10
DNA-binding region of the simian virus 40 tumor antigen.
... Finally, complexes consisting of SV40 DNA and T-antigen fragments were precipitated in the DNA-binding assay with monoclonal antibodies that recognize the central region of the protein; [r] ... See full document
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Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... resistance and subsequent establishment of clones as described previously (87, 89). To analyze small-t-induced phenotypic progression of these cells, small-t-coexpressing cell lines were generated by ... See full document
9
Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
... Analysis of A protein binding to a series of nonviable mutanfts progressively deleting these contact sites leads to the following conclusions: i stable binding of subunits of A protein t[r] ... See full document
5
Structure and synthesis of a simian virus 40 super T-antigen.
... DISCUSSION In productively infected permissive cells, the early region of SV40 DNA is transcribed into two mRNAs of 2.6 and 2.3 kb, which encode proteins of apparent molecular weights 17[r] ... See full document
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