[PDF] Top 20 Structure and function of the Epstein-Barr virus BZLF1 protein.

... DISCUSSION Deletion analysis of the BZLF1 protein has shown that the part of the protein between the BsmI and Hincll sites is required for sequence-specific DNA binding by BZLF1 at the s[r] ... See full document

... The sequence complementarity between region I and helix 36 of 18S rRNA is important for Zluc translation. Computer analysis indicates that the intercistronic region may form two large hairpin structures, SI and SII (Fig. ... See full document

... origin-binding protein. Origin-binding proteins may function to open chromatin, al- lowing formation of the replication ...leukemia protein (PML)-containing nuclear bodies ...simplex virus and ... See full document

... protein expression level and/or DNA binding activity. Interest- ingly, the N211A mutation produced a fivefold reduction in DNA replication, perhaps reflecting the central position of N211 within a hydrogen bond ... See full document

... 1 protein and the EBV EBNA1 protein both bind to mitotic chromosomes and seemingly link viral replication and segre- gation during the cell cycle (3, 4, 9), Z may also play a role in segregating replicating ... See full document

... simplex virus (HSV) and cytomegalovirus (CMV) are known to modify promyelocytic leukemia (PML) (ND10) bodies in the nucleus of the host cell, it has been unclear whether lytic infection with gamma herpesviruses ... See full document

... the BZLF1 promoter, we wished to determine the role this element plays in regulating expression of the BZLF1 ...the BZLF1 promoter into a luciferase reporter ...affect protein binding had ... See full document

... To examine the effects of exogenous ZEB in the absence of the complication of endogenous ZEB, we repeated these co- transfection experiments using MCF-7 cells. MCF-7 cells are a mammary epithelial cell line that lacks ... See full document

... Cells and antibodies. EBV-positive Burkitt’s lymphoma-derived P3HR-1 cells and the BJAB EBV-negative lymphoma cell line previ- ously described (30, 31) were a gift from Erle Robertson (University of Pennsylvania) and ... See full document

... BZLF1 (Z) and BRLF1 (R) not only bind to and transactivate early gene promoters, but they also modu- late the host’s intracellular environment by interacting with and altering the activities of many cellular ... See full document

... serine/threonine protein kinase and is the only protein kinase identified in the EBV genome (2, 3, 13, 14, ...viral protein kinase in the EBV life cycle is largely ...this protein kinase in ... See full document

... Distinct patterns of cellular factor binding to the ZI do- mains. EMSAs employing double-stranded oligonucleotides containing a single copy of each ZI element and nuclear extract prepared from the DG-75 cell line ... See full document

... of BZLF1 is mutated to a charged His residue in type 2 EBV strains and the dominant type 1 EBV strains in ...the BZLF1 EBV ...the BZLF1 protein ... See full document

... The Epstein-Barr virus (EBV) predominantly establishes latent infection in B cells, and the reactivation of the virus from latency is dependent on the expression of the viral BZLF1 ... See full document

... The corresponding amino acids are conserved in the basic domain of ZEBRA exon II, and proteins mutated in these positions were unable to bind any of the DNA-binding sites MS-AP-1, ZIIIA,[r] ... See full document

... An Epstein-Barr virus (EBV) protein microarray was used to screen for proteins binding noncovalently to the small ubiquitin- like modifier ...conserved protein kinase ...fluorescent ... See full document

... When we raised the MOI to 100 GRU per 10 4 cells, we detected proliferating B-cell colonies in 0, 1, and 4 wells of three 96-well plates of cells infected with the triple mutant on three different days. We were able to ... See full document

... Epstein-Barr virus (EBV) is a human herpesvirus associated with various ...genes, BZLF1 and ...of BZLF1 resides within the 3= UTR of BRLF1, miR-BART20-5p interacted with the 3= UTR of ... See full document

... the Epstein-Barr virus BZLF1 gene P1 promoter has been described ...the protein-binding sites (previously designated site I and site ...a protein which binds to both of these ... See full document

... The BZLF1 and BRLF1 adenoviruses induce necrosis and apoptosis in C18 NPC tumor ...the BZLF1, BRLF1, and GFP adenovirus vectors in C18 tumors, we performed histochemical analysis of tumors harvested 2 days ... See full document