[PDF] Top 20 Temperature-sensitive mutants in herpes simplex virus type 1 ICP4 permissive for early gene expression.
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Temperature-sensitive mutants in herpes simplex virus type 1 ICP4 permissive for early gene expression.
... Fifteen to fifty percent of wild-type levels of viral DNA are synthesized at the nonpermissive temperature in ts48- and ts303-infected cells, indicating that immediate-early and early ge[r] ... See full document
10
The N Terminus and C Terminus of Herpes Simplex Virus 1 ICP4 Cooperate To Activate Viral Gene Expression
... DNA-binding-deficient virus n12, which contains only the first 251 amino acids of the ICP4 protein, did not produce a viral yield higher than the initial inoc- ulum and was reduced by more than 4 orders of ... See full document
13
The Initiator Element in a Herpes Simplex Virus Type 1 Late-Gene Promoter Enhances Activation by ICP4, Resulting in Abundant Late-Gene Expression
... of ICP4 acti- ...in virus infection, has mutations at ⫺2, ⫺1, and ...LS mutants 7 and 10 were also substantially af- fected in all the assays, and these have mutations at ⫺2 and ⫹1 and ... See full document
11
Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein
... the permissive temperature of 33°C for the time in hours shown on the abscissas, cells together with the culture media were harvested and frozen at ⫺ ...the permissive temperature for the ... See full document
6
Transcriptional Coactivators Are Not Required for Herpes Simplex Virus Type 1 Immediate-Early Gene Expression In Vitro
... IE gene ex- pression, we repeated the low-MOI experiments in the pres- ...IE gene expression is not impaired in SiHa cells but is augmented in the presence of wild-type ...of 1 ... See full document
14
Initial characterization of the membrane-associated form of ICP4 of herpes simplex virus type 1.
... immediate-early gene product, ICP4, of herpes simplex virus type 1 (HSV-1) is one of the major transcriptional regulatory proteins in the virus ... See full document
5
Repression of gene expression upon infection of cells with herpes simplex virus type 1 mutants impaired for immediate-early protein synthesis.
... npt gene controlled by the ICP0 promoter ...lanes 1 and ...npt gene was responsive to VP16 in the transformed ...npt gene controlled by the ICP0 promoter, using the same plasmid to construct ... See full document
7
Evidence that the Immediate-Early Gene Product ICP4 Is Necessary for the Genome of the Herpes Simplex Virus Type 1 ICP4 Deletion Mutant Strain d120 To Circularize in Infected Cells
... linear herpes simplex virus (HSV) genomes may change into an “endless” or circular ...protein ICP4 is involved in the process of converting the linear HSV-1 ICP4-deleted mutant ... See full document
9
ICP0 Gene Expression Is a Herpes Simplex Virus Type 1 Apoptotic Trigger
... That herpes simplex virus type 1 (HSV-1) induces apoptosis but then prevents its killing of infected cells is ...a temperature-sensitive virus at its ... See full document
12
ICP27 Phosphorylation Site Mutants Are Defective in Herpes Simplex Virus 1 Replication and Gene Expression
... an early stage during infection with ICP27 phos- phorylation site ...of ICP4 were only mod- estly decreased, but ICP4 remained diffusely localized through- out the nucleus, with only a few ring-like ... See full document
12
Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.
... Regulation of the herpes simplex virus type 1 late gamma2 glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation [r] ... See full document
7
Molecular Genetics of Herpes Simplex Virus VI. Characterization of a Temperature-Sensitive Mutant Defective in the Expression of All Early Viral Gene Products
... KNIPE,' WILLIAM BATTERSON,' CATHY NOSAL, BERNARD ROIZMAN,2* AND Received 14 April 1980/Accepted 19 January 1981 The herpes simplex virus 1 HFEM mutant tsB7 failed to express any detectab[r] ... See full document
9
Cross-reactivity of the anti-pml antibody PG-M3 with the herpes simplex virus type 1 immediate early protein ICP4
... viral gene transcription, the immediate early protein ICP0 is produced, which then associates with ND10 structures and mediates their disruption (Everett & Maul, 1994 ; Maul & Everett, 1994 ; Maul ... See full document
5
Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4.
... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document
13
Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein.
... 354-366 0022-538X/83/010354-13$02.00/0 Copyright C 1983, American Society for Microbiology Genetic Analysis of Temperature-Sensitive Mutants Which Define the Gene for the Major Herpes Si[r] ... See full document
13
Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175.
... Consistent with this model are the following observations: i certain late proteins e.g., VP154 can be expressed in the absence of functional VP175 this study, ii VP175 is required for ce[r] ... See full document
15
DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4
... WT ICP4. In addition, a specific ICP4 binding site and a downstream nonspecific stretch of DNA were required for ...of ICP4 interacting with each ...the ICP4 binding site attests to the ... See full document
8
Requirement of the N-Terminal Activation Domain of Herpes Simplex Virus ICP4 for Viral Gene Expression
... wt ICP4 can be isolated in complexes with these factors ...of ICP4 are important for interac- tions with TFIID and mediator, affinity purification assays using TAP-tagged d3-10-expressing cells were ...wt ... See full document
9
Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants
... A DGGE gel showing 20 samples of PCR fragment II het- eroduplexes is presented in Fig. 1A. This denaturing gradient gel contained 50 to 65% of denaturants and was electropho- resed for 7 h. Of the 20 tk mutants, ... See full document
7
Expression of herpes simplex virus type 1 glycoprotein D deletion mutants in mammalian cells.
... First, each contained highermolecular-weight or aggregated forms; second, processing of the N-linked oligosaccharides to the product form was markedly reduced and no 0-linked oligosaccha[r] ... See full document
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