[PDF] Top 20 Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
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Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
... Therefore, to analyze further the mechanism of transformation, we have tried to isolate mutants of SV40 i which are temperature sensitive in their ability to transform nonpermissive rat [r] ... See full document
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Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ... See full document
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Characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40.
... In addition, the role of the gene A product if any in the maintenance of the transformed 1106 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Seven lines derived from p[r] ... See full document
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DNA Binding Properties of Simian Virus 40 Temperature-Sensitive A Proteins
... The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited footprint assay or excess fragment assay amounts of DNase I.. At l[r] ... See full document
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Biological and biochemical studies of cells transformed by simian virus 40 temperature-sensitive gene A mutants and A mutant revertants.
... Virus recovered from both of the tsA30-transformed cell lines was temperature sensitive for viral DNA synthesis in BSC-1 cells, as is characteristic of SV40 A mutants Table 2.. That reco[r] ... See full document
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Temperature-sensitive BC mutants of simian virus 40: block in virion assembly and accumulation of capsid-chromatin complexes.
... In addition, we have tested the state of the VP1 protein produced in cells infected with selected mutants of the BC group tsBC216, tsBC223, or tsB248 and found that a stable form of this[r] ... See full document
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Separation of lytic and transforming functions of the simian virus 40 A region: two mutants which are temperature sensitive for lytic functions have opposite effects on transformation.
... 518-528 0022-538X/81/050518-11$02.00/0 Separation of Lytic and Transforming Functions of the Simian Virus 40 A Region: Two Mutants Which Are Temperature Sensitive for Lytic Functions Hav[r] ... See full document
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Temperature dependency for maintenance of transformation in mouse cells transformed by simian virus 40 tsA mutants.
... The great majority of mouse embryo fibroblast transformants generated with tsA mutant virus were temperature sensitive for transformation in all three assays, regardless of the input mul[r] ... See full document
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DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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Temperature-Sensitive Mutants of Simian Virus 40: Infection of Permissive Cells
... Plaque characteristics of wild-type and mutant simian virus 40 SV40 stocks propagated at 32 C Virus Efficiency of plating39 C/32 C Time of plaque appearance in days d Infected cultures w[r] ... See full document
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Products of complementation between temperature-sensitive mutants of simian virus 40.
... These results imply that i the D product may be synthesized late in infection; ii therefore, any defect exhibited by D virions early in infection probably results from the introduction o[r] ... See full document
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Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ... See full document
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Simian virus 40 mutants carrying two temperature-sensitive mutations.
... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ... See full document
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Isolation and Characterization of Sendai Virus Temperature-Sensitive Mutants
... From these data it is apparent that mutants belonging to complementation groups D, E, and F are defective in functions required for virus-specific RNA synthesis throughout the replicativ[r] ... See full document
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Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ... See full document
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Biochemical characterization of temperature-sensitive rabies virus mutants.
... Production of viral complementary RNA in cells infected by wild-type rabies virus at 39.60C in the presence or absence of cycloheximide.. Total unlabeled RNAs were extracted from rabies-[r] ... See full document
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Isolation and characterization of temperature-sensitive mutants of measles virus.
... Other experiments data not shown have indicated that cells infected with ts-8 or ts-9 at 39 C reproducibly yield significant amounts of virus-specific RNA, but the amount of RNA synthesi[r] ... See full document
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Maturation Defects in Temperature-sensitive Mutants of Sindbis Virus
... Viral materials from cells infected with the mutants under nonpermissive conditions were compared with those from cells infected with wild-type virus, in terms of i the distribution of t[r] ... See full document
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Isolation of Temperature-Sensitive Mutants of Murine Sarcoma Virus
... Hybridization was Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Three separate murine sarcoma virus nonproducer cell lines have been isolated which are temperature se[r] ... See full document
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