[PDF] Top 20 The temporal and spatial analysis of single cell gene expression
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The temporal and spatial analysis of single cell gene expression
... the analysis of the spatial organisation of different cell types within the ...different cell types during ...two cell types emerge from a single undifferentiated pop- ulation or ... See full document
257
Spheroid arrays for high throughput single cell analysis of spatial patterns and biomarker expression in 3D
... Marker expression can not only be quantified, but also localized within the ...in expression due to the low coefficient of ...the spatial distribution of a biomarker expressed in a small population ... See full document
12
Spatial-Temporal Patterns of Viral Amplification and Interference Initiated by a Single Infected Cell
... images of the plaques using detection of maxima. The area of each plaque was quantified at each time point based on the fluorescence-positive area. The area equivalent radius (AER) of the measured area was calculated by ... See full document
15
Developmental fate and lineage commitment of singled mouse blastomeres
... component analysis (PCA) based on the expression of these genes in the TE, ICM and 1/32LS ...blastomere expression patterns onto component 1 and 2 clearly separated the individual cells into three ... See full document
10
Characterizing heterogeneity in leukemic cells using single cell gene expression analysis
... and analysis of single- cell data ...analyze single-cell gene expression data, using the identified cellular hierarchy as a ...Our analysis identified a core module ... See full document
13
ZIFA: Dimensionality reduction for zero inflated single cell gene expression analysis
... latent expression val- ues for zero measurements. If a gene has a very low frequency of expression and is zero across most cells, this imputation process is unlikely to yield further information and ... See full document
10
Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
... components analysis projection of the 91 cells largely segregates the five cell types (Figure ...examined expression levels for a curated list of marker mRNAs expected in serotonergic neurons, ... See full document
136
Single cell transcriptional profiles and spatial patterning of the mammalian olfactory epithelium
... other cell-intrinsic differences of OSNs are likely to represent a much higher percentage (Figure 1B), we observed that a large portion of candidate zone-specific transcripts represented false-positive ... See full document
7
A spatial temporal analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic wheat infection
... Affymetrix expression data were RMA and MAS5 normalized and extracted using the Affymetrix Expression ...Information-Gene Expression Omnibus (NCBI-GEO) under the accession number ... See full document
19
Determining Physical Mechanisms of Gene Expression Regulation from Single Cell Gene Expression Data
... to cell-to-cell ...throughput single cell gene expres- sion assays (single cell qPCR and ...generate gene expression distributions which can be used to ... See full document
29
Spatial and temporal patterns of gene expression during neurogenesis in the sea urchin Lytechinus variegatus
... promote cell proliferation in the early embryo ...of expression of Lv-elk in the apical organ at pluteus stages suggests that it could have a role in the differentiation of neurons there, similar to its ... See full document
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TAEL: a zebrafish optimized optogenetic gene expression system with fine spatial and temporal control
... a single endodermal layer adjacent to the yolk, whereas in activated embryos (N), GFP-positive cells are located in multiple cell layers, especially in the outer EVL and ectoderm ... See full document
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Simultaneous Single-Cell In Situ Analysis of Human Adenovirus Type 5 DNA and mRNA Expression Patterns in Lytic and Persistent Infection
... and Cell Profiler software (48). Briefly, each cell nucleus was identified and separated based on shape features using a custom- made ImageJ ...into Cell Profiler, and cell area and cytoplasm were ... See full document
17
Quantification of cell identity from single cell gene expression profiles
... 15 cell types queried; Figure 4C), indicating a quanti- fiable loss or decrease in cell ...Indeed, expression of most meristematic xylem markers was reduced, although still present (Figure S5A in ... See full document
12
SCANPY: large scale single cell gene expression data analysis
... of cell types are carried out, providing speedups between 5 and 90 times in each step (https:// ...an analysis of data sets with more than one million cells and an interactive analysis with run times ... See full document
5
Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
... The mechanisms by which Notch signaling induces the T-fate choice are still under investigation. Thymic stimulation of the Notch pathway is mediated by the binding of the Delta-like ligand to the Notch1 receptor. Notch ... See full document
225
Spatial and Temporal Analysis of Alphavirus Replication and Assembly in Mammalian and Mosquito Cells
... for spatial and temporal regulation of the alphavirus life cycle in mammalian host and insect vector ...the cell surface glycoproteins generate virions that utilize the filopodial extensions for viral ... See full document
16
Spatial and temporal activity of the dentin sialophosphoprotein gene promoter: differential regulation in odontoblasts and ameloblasts
... of gene regulation are not clearly ...reporter gene (lacZ) under the control of ~6 kb upstream sequences of ...of cell type and developmental stages at which the Dspp-lacZ gene is ...specific ... See full document
8
Gene Regulatory Networks Controlling Multicellular Development.
... Between 300-500mg of AGL42:GFP 30 seed were wet sterilized and plated (see Growth conditions) for each biological replicate. After growth, approximately 1mm of the root tip was collected and protoplasted as described in ... See full document
340
Temporal and spatial expression patterns of Hedgehog receptors in the developing inner and middle ear
... examined expression patterns of Hh co- receptors in the neonatal inner ear together with that of the hair cell marker atonal bHLH transcription factor 1 (Atoh1) ...Gas1 expression patterns in the ... See full document
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