[PDF] Top 20 Typing of Human Enteroviruses by Partial Sequencing of VP1
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Typing of Human Enteroviruses by Partial Sequencing of VP1
... EV typing, it is labor-intensive and time-consuming and may fail to identify the serotype of a clinical isolate because of antigenic drift, recombination, or the presence of virus mixtures in the specimen being ... See full document
6
Molecular Identification and Analysis of Nonserotypeable Human Enteroviruses
... proteins, VP1 to VP4: VP1, VP2, and VP3 are located at the surface of the viral capsid and are exposed to immune pressure, whereas VP4 is located inside the capsid ...protein VP1 contains the ... See full document
7
Molecular Evolution of the Human Enteroviruses: Correlation of Serotype with VP1 Sequence and Application to Picornavirus Classification
... the VP1 sequence can be used to classify viruses within the family Picornaviridae ...3). Enteroviruses of the same serotype were clearly distinguished from those of heterologous serotypes, and the limits of ... See full document
8
Typing of Human Enterovirus by Partial Sequencing of VP2
... Typing of well-characterized strains of HEV by VP2 se- quencing. A total of 61 field isolates, most of them belonging to the HEV-B species, were selected because of their previous identification at the serotype ... See full document
10
Simultaneously Typing Nine Serotypes of Enteroviruses Associated with Hand, Foot, and Mouth Disease by a GeXP Analyzer Based Multiplex Reverse Transcription PCR Assay
... by enteroviruses, which are members of the picornavirus family (single-stranded RNA, non- enveloped), and is most commonly associated with coxsackievirus A16 (CVA16) and human enterovirus 71 (EV71) ...and ... See full document
6
Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... the VP1 gene can func- tion as an excellent surrogate for serotyping by ...a partial or complete VP1 nucleotide sequence identity of ⱖ 75% ( ⬎ 85% aa se- quence identity) between a clinical EV ... See full document
7
Detection and Genetic Characterization of Enteroviruses Circulating among Wild Populations of Chimpanzees in Cameroon: Relationship with Human and Simian Enteroviruses
... of enteroviruses infecting ...minimal human contact in Cameroon were screened for ...the VP1, VP4, and partial VP2 genes, the 5 ⴕ untranslated region, and partial 3Dpol sequences ... See full document
7
Molecular Identification and Typing of Enteroviruses Isolated from Clinical Specimens
... amplicon sequencing, following a previously published protocol ...the VP1 of a large range of EVs were used in the order 012-011 and then 187-222 (22, ... See full document
7
Analysis of the Serotype and Genotype Correlation of VP1 and the 5′ Noncoding Region in an Epidemiological Survey of the Human Enterovirus B Species
... Molecular typing by sequence analysis and phylogenetic ...the VP1 gene, 342 Belgian patient sam- ples collected over a period of 4 years were diagnosed as being enterovirus positive and subsequently were ... See full document
9
Valine/isoleucine variants drive selective pressure in the VP1 sequence of EV A71 enteroviruses
... population. Furthermore, this 2011–2012 outbreak was also characterized by the cocirculation of the same four variant populations with a replacement between 2011 and 2012. The VII and IIV variants which were the most ... See full document
12
Identification of enteroviruses in clinical specimens by competitive PCR followed by genetic typing using sequence analysis
... by partial sequencing of the VP2 region of virtually all of the enterovirus prototype strains that this clustering could be used as the basis of molecular classification of the whole group (14, 25), ... See full document
6
Human rhinovirus infection in young African children with acute wheezing
... [13,21,37]. Typing using the 5’UTR may underestimate the true number of HRV-C samples as there is evidence that recombination with HRV-A spe- cies at this site is a common occurrence, resulting in these types ... See full document
8
Constituents of SH1, a Novel Lipid-Containing Virus Infecting the Halophilic Euryarchaeon Haloarcula hispanica
... proteins VP1, VP4, and VP7 form stable ...proteins VP1 (calculated molecular mass, ...a VP1 trimer and dimer, respectively, they most probably represent such ... See full document
11
Multilocus Sequence Typing System for Group B Streptococcus
... The aim of this work was to establish an MLST typing system, but there were sufficient numbers of strains to make early observations about the population structure of GBS. The most common STs in the data set were ... See full document
7
Effect of partial replacement of cement by fl...
... The effect of fly ash , ACBFS and silica fume on compressive strength and flexural strength of concrete proportioned with various levels of cement replacement i.e. A r[r] ... See full document
6
Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... discriminatory and unambiguous method of characterizing bac- terial isolates that has now been successfully employed in the char- acterization of several bacterial species, including Mycoplasma hyopneumoniae, Mycoplasma ... See full document
8
Genetic Diversity of Neisseria gonorrhoeae Housekeeping Genes
... or sequencing primers (not shown) and half the amount of the manufacturer’s recommended BigDye Ready Reaction mix (PE Biosystems, Foster City, ...and Sequencing Facility, Department of Biological Chemistry, ... See full document
8
Identification of Helicobacter pylori and OtherHelicobacter Species by PCR, Hybridization, and Partial DNA Sequencing in Human Liver Samples from Patients with Primary Sclerosing Cholangitis or Primary Biliary Cirrhosis
... subsequent sequencing of a part of the amplified ureA gene, ...7 human bile samples collected by percutaneous transhepatic cholan- giodrainage from patients with pancreatic head tumors, sug- gesting that ... See full document
5
Molecular Identification of GemellaSpecies from Three Patients with Endocarditis
... and sequencing of the PCR ...for sequencing by using Microspin S-400 HR columns (Pharmacia Biotech, Uppsala, Sweden) according to the manufacturer’s ...Amplicycle sequencing kit (Per- kin-Elmer), ... See full document
6
A Pilot study on molecular typing and seroepidemiology of Human enteroviruses (HEV) in aseptic meningitis and some other common clinically associated diseases
... of human and mammalian diseases. They are named enteroviruses because of their major route of transmission by the faecal-oral route and they reside and multiply in the gastrointestinal ...major human ... See full document
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