Eukaryotic expression
Roupelieva, Maria (2005): ORFeome-based arrays in eukaryotic expression vectors - a new approach to screen for the function of viral proteins: LANA-1 meets the mediator. Dissertation, LMU München: Fakultät für Biologie
... PCR products flanked by the attB sites for recombination were generated of all KSHV ORFs and cloned into the entry vector pDONR207 by BP clonase (Uetz et al. unpublished). 114 DNA fragments representing full-length or ...
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Construction of a recombinant eukaryotic expression vector containing <em>DNM3</em> gene and its expression in colon cancer cells
... recombinant eukaryotic expression vector, and stable DNM3 expression was observed in SW620 and LoVo cell ...protein expression of MMP-2 and ...the expression of MMP-2 and ...
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Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells
... Prolyl hydroxylase domain 3 (PHD3) is a hypoxia inducible factor- α (HIF α ) regulator; it degrades HIF α in the presence of oxygen. Recently, there have been an increasing number of studies about the role of PHD3 in ...
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Construction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen
... Both pcDNA3.1-LHDAg and pIRES2-EGFP- LHDAg expressed L-HDAg in both cell lines, although after trying several times, each time the transfection efficiency of Huh7 cells[r] ...
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Expression and immunogenicity of erythropoietin gene cloned in eukaryotic expression vector (pVAX1 vector)
... the expression of epo is generally low, meaning that many donors are necessary to obtain sufficient material for ...subsequent expression in Chinese hamster ovary (CHO) cells led to the production of ...
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Expression and immunogenicity of H glycoprotein gene of canine distemper virus cloned in eukaryotic expression vector
... The immune response on 30th day post vaccination induced by 4µg dose of rplasmid revealed protective serum neutralizing antibody titer 120, while control group did not show any antibody [r] ...
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Expression of Influenza Heamagglutinin Globular Head in Different Eukaryotic Cells
... The HA1 gene sequence (996 bp) has been cloned into pFastBac vector by Yousefi et al (11). In this study the HA1 gene sequence was obtained from pFastBac-HA1 by digestion and subcloned into the eukaryotic ...
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The human T-cell lymphotropic virus type 1 Tof protein contains a bipartite nuclear localization signal that is able to functionally replace the amino-terminal domain of Rex.
... Rex-dependent expression of Tof from the 1-2-B ...with eukaryotic expression plasmids containing the Tof coding sequence and driven by the HIV-1 LTR (8) or human cytomegalovirus promoter ...Tax ...
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A DNA Vaccine Encoding for TcSSP4 Induces Protection against Acute and Chronic Infection in Experimental Chagas Disease
... Immunization of mice with plasmids containing genes of Trypanosoma cruzi induces protective immunity in the murine model of Chagas disease. A cDNA clone that codes for an amastigote-specific surface protein (TcSSP4) was ...
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Translation initiation events on structured eukaryotic mRNAs generate gene expression noise
... gene expression noise in eukaryotes (predominantly in the form of high-throughput genome-wide studies) has emphasized the influence of vari- ations in mRNA copy number per cell that are driven by fluctuations in ...
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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
... recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ...omnipotent ...
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Heterologous expression of plasmodial proteins for structural studies and functional annotation
... host expression system can be optimized to ensure expression plasmid stability, the absence of harm- ful natural proteases and availability of the relevant genetic elements, ...the expression and ...
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Expression of Gp63 Gene from NIH Strain of Leishmania major in Pichia pastoris
... Four positive clones were selected and cultured in the media as described in the materials and methods. The expression of rgp63 in induction media, cell pellet and supernatant of the sonicated cells was studied by ...
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DNA
... • Control elements and the transcription factors they bind are critical for the precise regulation of gene expression in different cell types. – To initiate transcription, eukaryotic R[r] ...
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Cloning and functional expression of the first eukaryotic Na+–tryptophan symporter, AgNAT6
... The expression of all seven AgNATs, nine AeNATs and six DmNATs was confirmed by molecular cloning from specific cDNA collections (all 22 clone sequences are available in the NCBI database, see ...heterologous ...
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AP Biology Yearlong Outline and Assignments Semester 1.docx
... Quiz #13 Eukaryotic gene expression.. 41i[r] ...
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Cloning of cDNA for murine interleukin-3 and its expression in eukaryotic and prokaryotic systems
... cDNA clones coding for human were isolated methods clones The fr o m a to those used Taniguchi human IL-2 et al., Devos al., et al., 1983; from 1983 1983 library to isolate mRNA Taniguch[r] ...
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Clusters of co expressed genes in mammalian genomes are conserved by natural selection
... 2000). These co-expressed genes cannot be operons, because the two genes often occur on opposite strands of DNA, mak- ing polycistronic transcription impossible (Cohen et al., 2000). The same co-expression of ...
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A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression
... Transient expression of CpBV-H4 and a truncated CpBV-H4 in Tribo- lium ...transient expression of CpBV-H4 and truncated ...(B) Expression analysis by RT-PCR, in which primers were designed at an ...
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Dobreva, Gergana (2004): Identification and functional characterization of the novel MAR-binding protein, SATB2. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... In conclusion, it should be pointed out that although a lot has been learned about the function of MARs, the precise mechanism through which they influence gene expression is still obscure and probably diverse. ...
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