Expression vector
Expression Enhancement of Trastuzumab in CHO Cells Using Codon Optimization and Promoter Selection for Mammalian Expression Vector
... DHFR expression cassette of pBG-SVII ...(pgk) expression vector, expressed efficient and had a titer of ...The expression vector optimization by selecting appropriate promoter for DHFR ...
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Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa
... pmKate2–N expression vector were digested with SacI and SacII restriction enzymes by incubating for 30 min at 37 °C (Jean Bioscience GmbH, Germany) as described by Kuspa ...pmKate2–N vector in a re- ...
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Replication-Defective Bovine Adenovirus Type 3 as an Expression Vector
... Bovine adenoviruses (BAVs) belong to the Mastadenovirus genus of Adenoviridae family. Currently, the accepted 10 sero- types of BAVs are divided into two subgroups on the basis of the differences in their biological and ...
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Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells
... Prolyl hydroxylase domain 3 (PHD3) is a hypoxia inducible factor- α (HIF α ) regulator; it degrades HIF α in the presence of oxygen. Recently, there have been an increasing number of studies about the role of PHD3 in ...
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Clonning and characterization of alfa-globulin promoter into an expression vector
... intermediate vector, ...pCAMBIA1305.2 expression vector to produce the recombinant plasmid, pCAMGpro (~12,981 bp) followed by Bioinformatics analysis of the AsGpro ...
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Transgenic or Plant Expression Vector-Mediated Recombination of Plum Pox Virus
... Recombination between CP-defective PPV and transiently expressed CP genes of different PPV strains. The attempt to reproduce the restoration of assembly-defective PPV in trans- genic plants carrying an intact CP gene ...
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Construction, characterisation and utilisation of a novel E coli expression vector
... 1984). Expression was obtained in ...directed expression of CPG2 to between 1Z and ...of expression, compared to the native promoter ...The expression vector pNM21 in the host W5445, ...
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Construction and transformation of expression vector containing Panax japonicus SS gene.
... (SS) expression vector for the biosynthesis of TS was developed by genetic engineering technology; its transformation was examined, providing a foundation for molecular ...
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Construction of Double Gene Expression Vector
... larger vector. In order to overcome these problems a novel expression vector was developed with unique restriction sites and two different inducible promoters to control the expression of both ...
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Rev dependent lentiviral expression vector
... LTR-based expression vec- tor can mediate high levels of transcriptional activity in some cells [13], leading to expression from the reporter LTR in the absence of ...high expression cells can be ...
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Engineering the Transmissible Gastroenteritis Virus Genome as an Expression Vector Inducing Lactogenic Immunity
... TGEV vector is probably due to the known low immunogenicity of GFP (48, ...virus vector and elicited partial pro- tection against porcine respiratory and reproductive syndrome virus-induced disease ...
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Expression and immunogenicity of H glycoprotein gene of canine distemper virus cloned in eukaryotic expression vector
... The in vivo experimental setup was designed with sixteen one month age old pups. One week before the start of immunization trial, all pups were treated with antihelminthics (Albendazole). These dogs were then tested for ...
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Expression of Sindbis virus 26S cDNA in Spodoptera frugiperda (Sf9) cells, using a baculovirus expression vector.
... By immunoblot analysis with antisera directed against whole Sindbis virus and the individual structural proteins capsid, E2, and El, we have shown that polypeptides similar in size and a[r] ...
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Expression and immunogenicity of erythropoietin gene cloned in eukaryotic expression vector (pVAX1 vector)
... Cells were trypsinised using trypsin-versenate solution (TVS) and then 4 ml of GMEM containing 10% FCS and penicillin and streptomycin (50 µl/ml) was added, to make cell suspension of 1X105 cells/ml. Harvested ...
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Instruction Manual. Baculovirus. 6th Edition, May Expression Vector System
... the vector is chosen, the gene of interest is cloned into a restriction enzyme site downstream of the BV ...gene expression in the BV System can differ by approximately 1000 fold due to the intrinsic nature ...
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Vector algebra in the analysis of genome wide expression data
... reference expression vector of a wild-type strain treated with rapamycin and four other expression vectors are represented on a ...of vector magnitudes ...four expression vectors have a ...
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Foxtail mosaic virus: a new viral vector for protein expression in cereals
... Figure 2. Development of a FoMV expression vector. A: FoMV genome organisation and expression strategy. The viral genomic RNA (gRNA) contains five major open reading frames (ORFs; labelled from 1 to ...
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Inducible Overexpression of a Toxic Protein by an Adenovirus Vector with a Tetracycline-Regulatable Expression Cassette
... tTA expression and reporter plasmid ptTA-lux with the p39SS plasmid (Stratagene), which allows for hygromycin resistance ...tTA expression 2 days later by assaying cell extracts for luciferase activity with ...
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Expression and Purification of Human Interferon Gamma Using a Plant Viral Vector
... an expression vector in ...This vector may also be useful as a tool for in planta genomic studies, as it can express a gene with effective protein ...plant-virus expression system also ...
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Vector Quantization of Microarray Gene Expression Data
... gene expression data lies in the fact that LVQ has been used as a tool to minimize classification errors, by means of more appropriate discrimination between decision boundaries of classes ...
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