Functional complementation
Incomplete influenza virus: partial functional complementation as revealed by hemadsorbing cell count test.
... Functional complementation of ts-29 29 had an infectious titer of 5 x 108 PFU/ml with standard and incomplete uwild-type virus as when titrated at 36°C and 5 x 102 PFU/ml when revealed b[r] ...
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Functional complementation of UL3.5-negative pseudorabies virus by the bovine herpesvirus 1 UL3.5 homolog.
... The UL3.5 gene is positionally conserved but highly variable in size and sequence in different members of the Alphaherpesvirinae and is absent from herpes simplex virus genomes. We have shown previously that the ...
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Functional complementation of nuclear targeting-defective mutants of simian virus 40 structural proteins.
... Since what was observed in host cells during infection by polyomaviruses 10, 13, 19, 22, 23, 26 does not reveal the role of individual protein components for nuclear targeting, these obs[r] ...
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Partial Functional Complementation between Human and Mouse Cytomegalovirus Chemokine Receptor Homologues
... whether M33 plays a specific role during the establishment of, maintenance of, or reactivation from latency is not yet known. An in vivo model for HCMV-encoded 7TMR function would provide a useful system to determine ...
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Functional complementation of the adenovirus E1B 19-kilodalton protein with Bcl-2 in the inhibition of apoptosis in infected cells.
... When the Bcl-2 protein is expressed in HeLa cells during infection by mutant adenovirus deleted in the E1B 19K gene, any alterations in the induction of p53 levels by ElA may be attribut[r] ...
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Frequent Coinfection of Cells Explains Functional In Vivo Complementation between Cytomegalovirus Variants in the Multiply Infected Host
... In contrast to many other virus infections, primary cytomegalovirus (CMV) infection does not fully protect against reinfection. Accordingly, clinical data have revealed a coexistence of multiple human CMV variants/ ...
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Opaque7 Encodes an Acyl-Activating Enzyme-Like Protein That Affects Storage Protein Synthesis in Maize Endosperm
... ABSTRACT In maize, a series of seed mutants with starchy endosperm could increase the lysine content by decreased amount of zeins, the main storage proteins in endosperm. Cloning and characterization of these mutants ...
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Functional Evaluation of the Mechanisms of Reactive Oxygen Detoxification in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Complementation Studies
... It is known that anaerobic microorganisms can periodically be exposed to oxygen in their environments, and therefore, having some protective mechanism to detoxify reactive oxygen species would be highly advantageous for ...
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A substrate ambiguous enzyme facilitates genome reduction in an intracellular symbiont
... Functional complementation assays were also com- pleted to test the pantoate-β-alanine ligase (E.C. 6.3.2.1) activity of Buchnera panC. Buchnera panC shares only 48% amino acid sequence identity with E. ...
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Virus induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening
... in functional complementation of non-ripen- ing phenotypes and caused rin fruits to ripen ...Such complementation was unlikely to be caused by the recombination of homologous RNA ...
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The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing
... These analyses revealed that recombinant BAC clone 31O13, carrying the Cre10.g440000 gene and 12 other genes, restored the trans-splicing defect of mutant F19. Since Cre10.g440000 is most FIG 1 Functional ...
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Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis
... Pneumocystis spp. are extracellular opportunistic fungi that have been detected in the lungs of almost every mammalian species tested. Pneumocystis jirovecii, the species infecting hu- man beings, causes severe, often ...
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Complementation of diverse HIV 1 Env defects through cooperative subunit interactions: a general property of the functional trimer
... whether complementation could also be detected in a more bio- logically relevant situation, ...whether complementation can be detected not only by measuring Env-mediated cell fusion, but also virion ...
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Involvement of the Saccharomyces cerevisiae Hydrolase Ldh1p in Lipid Homeostasis
... that functional complementation of the ⌬ ldh1 mutant phenotype re- quires expression of enzymatically active Ldh1p, indicating that the hydrolase activity of the enzyme is required for its function in lipid ...
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Molecular genetics of sulphate assimilation in 'Arabidopsis thaliana'
... Functional complementation of the Escherichia coli cysteine auxotrophic strain JM15 serine acetyltransferase deficient using an Arabidopsis thaliana cDNA library in the expression.. memb[r] ...
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Complementation of Yeast Genes with Human Genes as an Experimental Platform for Functional Testing of Human Genetic Variants
... the functional con- sequence of human gene variants, we believe that comple- mentation of the null allele is more desirable mainly for two ...candidate complementation pairs, as each human– yeast gene pair ...
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Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris
... In this study, we used the α-peptide of β-galactosidase with the optimal length (1–92 amino acids) as the sec- ond reporter in bicistronic reporter vector to screen functional IRESes in P. pastoris. On total, ...
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Interallelic Complementation at the Drosophila melanogaster gastrulation defective Locus Defines Discrete Functional Domains of the Protein
... sequences or within the introns. The positions of the hypomorphic alleles. observed changes relative to the protein structure are Interallelic complementation analysis of gd alleles: summarized in Figure 1. ...
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Orientation-specific cis complementation by bulge- and loop-mutated human immunodeficiency virus type 1 TAR RNAs.
... Given that tandem HIV-1 TAR elements were functional, we then examined whether different mutated TAR elements could be combined in tandem to allow activation by complementation between t[r] ...
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Functional analysis of archaeal MBF1 by complementation studies in yeast
... performed complementation studies, it is tempting to speculate that aMBF1 might act as a single regulator or non-essential transcription factor, which directly interacts with DNA via the positive charged lin- ker ...
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