Isocratic hplc
Development and validation of a simple, quick and rugged isocratic HPLC-UV method for the detection of valsartan and hydrochlorothiazide in their combined pharmaceutical formulations
... C (ambient temperature). Resolution = 7.59, retention times was 2.3 min and 3.5 min for HCTZ and VAL respectively. The proposed method was tested for system suitability, linearity, range, precision, accuracy, ...
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Validation of a simple isocratic HPLC-UV method for rifampicin and isoniazid quantification in human plasma
... Freeze-thaw stability was determined in triplicate at 1, 2 and 3 cycles of freeze-thawing. At time zero, after taking an aliquot for quantification, the enriched plasma was separated into 2 mL Eppendorf tubes and frozen ...
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Development and validation of a simple isocratic HPLC method for simultaneous estimation of phytosterols in Cissus quadrangularis
... The plant material was collected from the campus of the Anand Agriculture University, Anand, Gujarat, India in August 2008. Plant specimen was authenticated at B. R. Doshi School of Biosciences, Sardar Patel University, ...
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Simultaneous RP HPLC estimation and validation of ramipril and atorvastatin in bulk and combined tablet formulation
... An isocratic HPLC with ultra violet detection method was successfully developed for the simultaneous analysis of ramipril and atorvastatin. The method validation results have proven that the method is ...
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Development and Validation of HPTLC and HPLC Methods for Simultaneous Determination of Closantel and Ivermectin in Veterinary Drug Products
... an isocratic HPLC method where separation was performed 13 on a C18 column using acetonitrile: methanol: 5mM ammonium dihydrogen phosphate buffer PH 6 (60:30:10, 14 by volume) as a mobile ...
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... phase, isocratic HPLC method was developed for the separation and quantification of pioglitazone and glimepiride in bulk drug and pharmaceutical dosage ...
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Determination Of Assay And Validation Of Stability Indicating RP-HPLC Method For Ganciclovir In Nciclovir Drug Substance
... and isocratic stability indicating reversed segment excessive performance liquid chromatography (RP-HPLC) technique have become advanced and showed for the willpower of assay of Ganciclovir in Ganciclovir ...
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HPLC Method for Determination of p-coumaric acid from the Medicinal Herb Leptadinia reticulata
... reported HPLC methods for of p- coumaric acid, especially those in the older literature, have several disadvantages, including unsatisfactory separation times, poor resolution and gradient ...rapid ...
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SIMULTANEOUS & STABILITY INDICATING METHOD FOR DETERMINATION OF CETRIZINE HYDROCHLORIDE AND AMBROXOL HYDROCHLORIDE IN SYRUP
... simple isocratic HPLC method has been developed and validated as per ICH Guidelines for simultaneous and stability indicating determination of Cetirizine Hydrochloride and Ambroxol hydrochloride in ...
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Optimization of HPLC method for the isolation of Hypericum perforatum L. methanol extract
... simple isocratic HPLC method was developed, allowing the baseline separation of thirteen main Hypericum perforatum standard compounds in less than 25 min, which means an improved separation 25-50 min less ...
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Development and Validation of RP-HPLC Method for the Determination of Methamphetamine and Propranolol in Tablet Dosage Form
... pH values. The best separation was obtained using the mobile phase consisted of a mixture of 50 mM pyrrolidine (pH 11.5) and acetonitrile (ACN) in ratio of (50:50, v/v). In reversed-phase HPLC, the pH of the ...
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Method Development and Validation for Simultaneous Estimation of Olmesartan Medoxomil and Hydrochlorothiazide by Rp-Hplc
... in any pharmacopoeia yet. Extensive literature survey revealed that very few methods were reported for the simultaneous estimation of OM and HCTZ. So, an attempt has been made to develop an accurate, precise and ...
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Development and validation of a simple and sensitive stability indicating RP HPLC assay method for determination of Nintedanib and stress degradation studies
... A Novel stability indicating Reverse Phase-Liquid Chromatography (RP-HPLC) method has been developed and validated for the determination of Assay of Nintedanib Drug Substance in the presence of degradation ...
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NOVEL METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN HYDROCHLORIDE AND OFLOXACIN BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP HPLC)
... Another advantage of this method is the use of an isocratic mobile phase of very simple composition, which gives the column a longer life time. From the results, it can be concluded that proposed method has been ...
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Validated Reverse Phase HPLC Method for The Determination of Pemetrexed Disodium in Pharmaceutical Dosage Forms
... The calibration curves were linear over a concentration range from 0.06248 mg to 0.4998 mg/ ml. Limit of detection (LOD) for Pemetrexed disodium was 0.00002522 mg/ml and Limit of quantitation (LOQ) Pemetrexed disodium ...
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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE AND HYDROCHLOROTHIAZIDE IN TABLETS: APPLICATION TO DISSOLUTION STUDY
... Also there are some analytical methods reported for determination of HCT alone and in combination. No method has been reported for the estimation of Amlodipine (AMLO) and Hydrochlorothiazide (HCT) in combined dosage form ...
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Analytical Method Development and Validation of Dutasteride and Tamsulosin Hcl in Combination and its Stress Degradation Studies
... Methanol HPLC grade of merck, NaOH AR grade, Potassium di hydrogen ortho phosphate AR grade, ortho phosphoric acid AR grade, Hydrochloric acid AR grade, Purified Water milli-Q water, were used for the analytical ...
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Determination of isotretinoin in pharmaceutical formulations by reversed phase HPLC
... thod using an isocratic high performance liquid chro- matography (HPLC) system to determine quantitatively the isotretinoin in hard gelatin capsule formulations. In order to validate an efficient method for ...
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AN ISOCRATIC, VALIDATED RP-HPLC TECHNIQUE FOR SIMULTANEOUS ESTIMATION OF DICLOFENAC AND NIFEDIPINE IN PHARMACEUTICAL FORMULATION
... A simple, rapid, and highly economical RP-HPLC method was developed for the simultaneous determination of model drugs chosen in the present study viz., diclofenac and nifidipine in their novel combined ...
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Development and Validation of an RP-HPLC Method for Gefitinib in Bulk and Pharmaceutical Dosage Forms using Central-Composite Design
... quantification limits were 10.47 mg/ml, and 31.74 mg/ml, respectively. The relative standard deviation was calculated as 0.4412%. Conclusion: Method developed and validated by determining its precision; accuracy; and ...
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