ITS1/5.8S rRNA/ITS2
Intervening Transcribed Spacer Region 1 Variability in Cyclospora cayetanensis
... SSrRNA, ITS1, 5.8S rRNA, ITS2, large-subunit rRNA, and the intergenic ...the ITS1 regions have less functional constraint than the SSrRNA in terms of sequence and therefore demonstrate ...
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The genetic variation of Angiostrongylus cantonensis in the People’s Republic of China
... Results: We characterised the complete mitochondrial genes cox1 and nad1 of 130 specimens and obtained 357 nuclear sequences containing two complete ITS (ITS1 and ITS2) and 5.8S rRNA of the same ...
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DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... available ITS1 and/or ITS2 sequences (for accession numbers, see Table ...carrying ITS1, the 5.8S rRNA gene, and ITS2 and varied in size from 375 bp (Candida pulcherrima) to 880 bp ...
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Genotyping of Pneumocystis jirovecii by Use of a New Simplified Nomenclature System Based on the Internal Transcribed Spacer Regions and 5 8S rRNA Gene of the rRNA Operon
... on ITS1-5.8S-ITS2 sequence ...jirovecii rRNA operon including the complete ITS1, 5.8S rRNA, and ITS2 ...product, 5 to 16 plasmid clones were randomly selected for Sanger ...
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Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method
... Yeasts are emerging as important etiological agents of nosocomial bloodstream infections. A multiplex PCR method was developed to rapidly identify clinically important yeasts that cause fungemia. The method ampli- fied ...
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Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei
... FIG. 1. Phylogenetic relationship of P. marneffei to Penicillium, Talaromyces, and Eupenicillium species inferred from combined nucleotide sequence of the internal transcribed spacers and 5.8S rRNA gene ...
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Multigene-Based Analyses of the Phylogenetic Evolution of Oligotrich Ciliates, with Consideration of the Internal Transcribed Spacer 2 Secondary Structure of Three Systematically Ambiguous Genera
... the ITS1-5.8S-ITS2 region was first applied to the study of oligotrichs and choreotrichs, it was suggested that the ITS and ...SSU rRNA gene and ...SSU rRNA gene and ...SSU rRNA gene ...
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Biogenesis and Function of Small Non-Coding RNAs Derived from Eukaryotic Ribosomal RNA
... phasi RNA (rRF) pathway 4 Piwi RNA (rRF) pathway 3 5’ 3’ 3’ 5’ rDNA rRNA rRNA cleavage rRFs 5’ 3’ 3’ 5’ 5’ 3’ 3’ 3’ 5’. 18S 28S 45S rRNA mmu-miR-712 5.8S ITS1 ITS2 TIMP3 mRNA induce endo[r] ...
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Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... The rRNA genes are good candidates, since they are present in high copy number and the sensitivity of their detection may be dramatically increased by the use of nested ...and ITS2) that are not translated ...
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Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds
... Using phenotypic methods alone, an isolate of Cladosporium spp. can be differentiated from Cladophialophora spp. in a time frame of 1 to 4 weeks. In addition to colonial and microscopic morphology, two tests are ...
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Update on Pneumocystis carinii f sp hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes
... differ in the numbers of T’s in the poly(T) tract, which is not used for typing in our study. These two types should be con- sidered the same and are equivalent to our type N. Type B3 is the same as our type J, except ...
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The molecular and morphometric identification of Dictyocaulus capreolus in clinically affected roe deer ( Capreolus capreolus(L.)
... In the present study, the SSU rRNA sequence analysis supported the morphological identifica- tion of the species as D. capreolus since the highest identity scores (99%) corresponded to D. capreolus from a roe deer ...
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Molecular phylogenetic history of eastern Mediterranean Alopiinae, a group of morphologically indeterminate land snails
... In order to evaluate the possibility of human-aided dispersal, divergence time can only be tentatively estimated, bearing in mind that these estimations rest on uncorroborated assumptions and may therefore be flawed. We ...
128
Phenotypic and Molecular Characterization of Madurella pseudomycetomatis sp nov , a Novel Opportunistic Fungus Possibly Causing Black Grain Mycetoma
... Here we present an extraordinary case of craniofacial eumy- cetoma extending from gum to lower jaw in a 27-year-old Chinese male. The case is worth reporting not only by its rareness in China but also its unusual ...
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Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus Positive Patients in Thailand
... Pneumocystis jirovecii is one of the common opportunistic infections in human immunodeficiency virus (HIV)-infected patients in Thailand. Information regarding genotypic and epidemiological of this organism in Thai ...
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Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
... We describe the development and evaluation of a novel, rapid MT-PCR assay for the detection and identification of fungi directly from positive blood cultures. The assay identifies seven Candida species (C. albicans, C. ...
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Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH)
... humuli, causing rose, tobacco, cucurbit, and hops downy mildew, respectively [28, 29]. However, to date, FISH assays have not been developed for species-specific visu- alization of oomycetes that cause downy mildew ...
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The trematode genera Paragonimus and Schistosoma in East Asia: molecular evolution, phylogeny and biogeography
... Alignment of ITS1 repeats from Asian schistosomes 44 47 Chapter 5 Intra - and inter- individual variation in ITS1 of Paragonimus westermani Trematoda: Digenea and related species Figure [r] ...
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Morphological and molecular characteristics of Malayfilaria sofiani Uni, Mat Udin & Takaoka n g , n sp (Nematoda: Filarioidea) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia
... During this investigation, we found filarial nematodes in common treeshrews captured in Kelantan, on the east coast of Peninsular Malaysia. None of the specimens collected match any of the hitherto described species or ...
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Rapid Identification of Dimorphic and Yeast Like Fungal Pathogens Using Specific DNA Probes
... placed into a preheated (96°C) Perkin-Elmer 9600 thermal cycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 4 min. The PCR product was then purified before sequencing using CentriSep spin columns ...
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