Moloney murine leukemia virus reverse transcriptase
RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity.
... The reverse transcriptase-associated RNase H activity of Moloney murine leukemia virus specifically cleaves within the polypurine tract region of the viral genome to generate the ...
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Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.
... in reverse transcription, we generated a panel of mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase based on sequence alignments and ...
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YADD Mutants of Human Immunodeficiency Virus Type 1 and Moloney Murine Leukemia Virus Reverse Transcriptase Are Resistant to Lamivudine Triphosphate (3TCTP) In Vitro
... Considerable progress has been made in the development of anti-human immunodeficiency virus type 1 (HIV-1) drugs and drug therapies. However, the emergence of drug-resistant viral strains presents a major problem; ...
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Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.
... AUSTIN2 Laboratory of Molecular Microbiology' and Laboratory of Immunopathology,' National Institute of Allergy and Infectious Diseases, and Developmental and Metabolic Neurology Branch,[r] ...
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Mutations of the RNase H C Helix of the Moloney Murine Leukemia Virus Reverse Transcriptase Reveal Defects in Polypurine Tract Recognition
... of Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (RT) and Escherichia coli RNase H possess a positively charged ␣ -helix (C helix) and a loop that are not present ...
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Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.
... If the entire plus strand is synthesized from one initiation event, as is generally depicted, our calculated rates suggest that displacement synthesis by the minus strand through the LTR[r] ...
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Structure-Based Moloney Murine Leukemia Virus Reverse Transcriptase Mutants with Altered Intracellular Direct-Repeat Deletion Frequencies
... of Moloney MLV reverse transcriptase reported at a ...ficiency virus type 1 (HIV-1) RT:Fab:DNA complex (19, ...render virus non- ...
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Expression of Moloney Murine Leukemia Virus RNase H Rescues the Growth Defect of an Escherichia coliMutant
... of Moloney murine leukemia virus reverse transcriptase encoding RNase H is shown to rescue the growth-defective phenotype of an Escherichia coli ...
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Reversion of a Moloney murine leukemia virus RNase H mutant at a second site restores enzyme function and infectivity.
... The reverse transcriptase of retroviruses contains an RNase H activity essential for the proper synthesis of the viral DNA copy of the RNA ...the Moloney murine leukemia virus ...
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Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein.
... Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.. Affinity purifica[r] ...
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Revealing Domain Structure through Linker-Scanning Analysis of the Murine Leukemia Virus (MuLV) RNase H and MuLV and Human Immunodeficiency Virus Type 1 Integrase Proteins
... the Moloney murine leukemia virus (M-MuLV) pol gene encoding the connection-RNase H domains of reverse transcriptase (RT) as well as the structurally related M-MuLV and human ...
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Replication Defect of Moloney Murine Leukemia Virus with a Mutant Reverse Transcriptase That Can Incorporate Ribonucleotides and Deoxyribonucleotides
... Reverse transcriptase (RT) plays a critical role in retrovirus replication, directing the synthesis of a double- stranded DNA copy of the viral RNA ...the Moloney mu- rine leukemia ...
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Abortive reverse transcription by mutants of Moloney murine leukemia virus deficient in the reverse transcriptase-associated RNase H function.
... Analysis of in vitro reverse transcription reactions carried out by mutant virions showed that minus-strand strong-stop DNA was formed but did not efficiently translocate to the 3' end o[r] ...
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Mutations in the gag gene of Moloney murine leukemia virus: effects on production of virions and reverse transcriptase.
... To explore the effects of these mutations on the utilization of the gag-pol precursor, we have introduced these mutants into cells already releasing defective particles from an endogenou[r] ...
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RNA-primed initiation of Moloney murine leukemia virus plus strands by reverse transcriptase in vitro.
... Each reaction contained, in addition to the standard salts mixture, [a-32P]dATP, 2,000 U of M-MuLV reverse transcriptase per ml and, as sources of template and potential primers, P190 fr[r] ...
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Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H.
... 615 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have studied a mutant Moloney murine leukemia virus with a deletion in reverse transcriptase RT which is predicte[r] ...
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Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase.
... 1 Isolation and Properties of Moloney Murine Leukemia Virus Mutants: Use of a Rapid Assay for Release of Virion Reverse Transcriptase Received 25 November 1980/Accepted 7 January 1981 A [r] ...
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Influence of DNA Methylation and Core Binding Factors in Expression of Leukemia Virus in Hemapoietic Stem Cells Manoj G. Tyagi 1* and Aniket Kumar1
... the Moloney murine leukemia virus (MLV) have been used due to their high efficiency of stable gene ...the Moloney murine leukemia virus (Moloney MLV) ...
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Nuclear factor 1 activates the feline leukemia virus long terminal repeat but is posttranscriptionally down-regulated in leukemia cell lines.
... For example, the enhancer domain of the Moloney murine leukemia virus Moloney MuLV LTR has binding sites for nuclear factor 1 NF1, the simian virus 40 core enhancerbinding protein, leuke[r] ...
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Further characterization of the Friend murine leukemia virus reverse transcriptase-RNase H complex.
... 418 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The purified reverse transcriptase-RNase H complex from Friend murine leukemia virus consists of a single polypeptid[r] ...
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