Normalization genes tested in qRT-PCR assays
Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1)
... key genes related to unknown pathways at the level of gene ...reference genes in this strain is essential for qRT‑PCR data normalization, although results have been reported for other ...
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Selection of reference genes for qRT PCR examination of wild populations of Atlantic cod Gadus morhua
... 10 genes group together in a PCA plot in intestinal ...reference genes can be used for normalization of qRT-PCR data across different wild populations of Atlantic ...ARP genes ...
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Original Article Validation of reference genes for the normalization of qRT-PCR expression studies in head and neck squamous cell carcinoma cell lines treated by different chemotherapy drugs
... family genes showed a high stability in both human breast cancer cell lines and FFPE biop- sies, but GAPDH performed poorly ...family genes performed poorly ...reference genes is con- text-dependent ...
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Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data
... housekeeping genes reported in model plant species due to the lack of genetic and sequence genome information ...keeping genes as reference gene in non-model plants can cause ...erence genes ...
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... reference genes for qRT-PCR in the Cactaceae ...reference genes were selected to determine the most stable refer- ence genes for qRT-PCR normalization in ...
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Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization
... The two regions of ITS1 and ITS2 are not part of the ribosome genes and are spliced and removed in mature rRNA. In our hypothesis, no amplification must be observed in DNase-treated RNA samples when two regions of ...
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Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
... transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and ...by normalization to one or more reference genes which ...
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Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
... suppressor genes lose their function ...by qRT-PCR. It is necessary to validate reference genes in the particular experimental system in order to trust the differential gene expressions which ...
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Analytical and clinical performance of a Chikungunya qRT PCR for Central and South America
... 2.15. Prospective sample collection Serum samples were obtained from 63 patients presenting at a fever clinic in Hospital Del fi na Torres Conchan in Esmeraldas, Ecuador, in July 2015 with symptoms b 10 days duration. All ...
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Selection of reference genes for normalization of quantitative real time PCR in organ culture of the rat and rabbit intervertebral disc
... reference genes (Actb, GAPDH, 18S rRNA, CycA, Hprt1, Ywhaz, and Pgk1) and attempted to identify the most stable control gene (s) for normalization of qRT-PCR data from rat and rabbit organ ...
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Accurate normalization of real time quantitative RT PCR data by geometric averaging of multiple internal control genes
... control genes, we subsequently defined a gene-stability measure M as the average pairwise variation between a particular gene and all other control ...housekeeping genes belonging to different functional ...
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The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype Specific PCR Assays
... were tested by hybridization of serotype 1, 2, or 9 cps sequences with chro- mosomal DNA of the reference strains of all known 35 sero- types of ...type-specific PCR assays for serotype 9, serotype 2 ...
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Development of PCR Assays Targeting the Genes Involved in Synthesis and Assembly of the New Escherichia coli O174 and O177 O Antigens
... associated genes were assigned functions on the basis of homology. Two genes, each specific for ...identified. PCR assays based on the O-antigen-specific genes were developed and ...
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Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis
... succino- genes was estimated by quantitative real time PCR (qRT-PCR) by amplification of specific 16S rDNA gene ...of qRT-PCR and DGGE were determined in preliminary ...
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: : : :HBV ". qrt-pcr
... 44. Bita Moudi. Epigenetics and genetic changes in CHD5 and risk of the Hepatocellular carcinoma. Gene, Cell and Tissue: 7 (3); e104222. 45. Z Heidari 1 2 , B Moudi 1 2 3 , H Mahmoudzadeh-Sagheb 1 2 . Expression Patterns ...
Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
... a normalization step of gene expression data is essential [9, 11–13] to correct varia- tions present at samples and conditions [13, ...reference genes for qPCR analyses, different mathematical algorithms ...
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DyNAmo cdna Synthesis Kit for qrt-pcr
... reference genes are β-actin, GAPDH and 18S ...housekeeping genes the expression usually varies to some ...reference genes are usually required, and their expression needs to be checked for each ...
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Table S1 qrt-pcr primers used in the study
... Table S1 qRT-PCR primers used in the study. No.[r] ...
Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
... transcription PCR (F-qRT-PCR)-based multi-fusion gene screening method with the capacity to accurately screen 22 common fusion genes concurrently without prior characterization of chromosome ...
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Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage
... housekeeping genes are combined together to form the BestKeeper ...of genes in terms of their ...ing genes, the authors recommended the use of the best 3 or 4 genes as that provides a ...
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