parvovirus H-1
Influence of the oncolytic parvovirus H-1, CTLA-4 antibody tremelimumab and cytostatic drugs on the human immune system in a human in vitro model of colorectal cancer cells
... of parvovirus H-1 (H-1PV) to efficiently kill colorectal cancer cells and induce immunogenicity of colorectal tumors by inducing matura- tion of dendritic cells (DCs) alone and also in ...
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Chimeric and Pseudotyped Parvoviruses Minimize the Contamination of Recombinant Stocks with Replication-Competent Viruses and Identify a DNA Sequence That Restricts Parvovirus H-1 in Mouse Cells
... to parvovirus infection over that of nontransformed cell cultures (6, ...rat parvovirus H-1 and the closely related minute virus of mice (MVM), can protect their host from developing tumors or ...
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Identification of a Novel Cellular TPR-Containing Protein, SGT, That Interacts with the Nonstructural Protein NS1 of Parvovirus H-1
... from parvovirus H-1 and which we termed SGT, for small glutamine-rich tetratricopeptide repeat (TPR)-containing ...In H-1 virus-infected rat and human cells, compared to mock- infected ...
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Defective interfering particles of parvovirus H-1.
... The variations in size due to the variable number of additions and deletions in the right half of the molecule caused the HindIII 51/100 fragments of the DI-wt and DI-wt-tsl4 preparation[r] ...
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Cytosolic Activation of Cathepsins Mediates Parvovirus H-1-Induced Killing of Cisplatin and TRAIL-Resistant Glioma Cells
... (47). H-1PV has been shown to block the gen- eration of reactive oxygen species in HeLa cells (32), and inhibition of cPLA2 by chemical agents has no effect on H-1PV-induced cell killing in gliomas (data ...
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Isolation of a fully infectious variant of parvovirus H-1 supplanting the standard strain in human cells.
... The H-1 virus life cycle in NB-E cells is controlled, in particular, by the NS-1 protein, which was found to be essential for both viral DNA replication and expression ...the H-1 dr ...
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Replication process of the parvovirus H-1. IX. Physical mapping studies of the H-1 genome.
... Double digestion with HaeIII and EcoRI or HaeII revealed that both EcoRI and HaeII cleaved only the HaeIII-A fragment 1,570 bp, thus establishing that both cleavage sites are adjacent to[r] ...
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Infectious process of the parvovirus H-1: correlation of protein content, particle density, and viral infectivity.
... Analysis of the protein content of these particles demonstrated that the ratio of viral protein VP2' to VP2 was the same in heavy-full and light-full particles derived from the same cell[r] ...
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Replication process of the parvovirus H-1. VII. Electron microscopy of replicative-form DNA synthesis.
... Also, the material remaining at the origin of the lanes containing DNA from fraction B of the sucrose gradient is presumed to be entangled molecules not greater than dimer size, since el[r] ...
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Replication process of the parvovirus H-1. X. Isolation of a mutant defective in replicative-form DNA replication.
... The increase cells infected with ts14 and H-3 as in Table 5, experiment 1 revealed about 60% of the cells in titer for LuIII as determined by PFU at to be positive for H-3 intranuclear a[r] ...
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Parvovirus H-1 expression: mapping of the abundant cytoplasmic transcripts and identification of promoter sites and overlapping transcription units.
... of Fragment HaeIII-B HaeIII-C HaeIII-D HaeIII-E HaeIII-F HaeIII-G HindII/III-A HindII/III-B HindIII-B Map units map unit for: nucleotides of predominant Leftward a-amanitin-sensitive Rig[r] ...
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Characterization of the trans-activation-responsive element of the parvovirus H-1 P38 promoter.
... Unlike the tar elements described for human T-cell lymphotropic virus types I and II or the glucocorticoid receptor element of mouse mammary tumor virus, which function at a distance, bi[r] ...
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Replication process of the parvovirus H-1 V. Isolation and characterization of temperature-sensitive H-1 mutants.
... Synthesis of the viral DNA strand was shown to be diminished for both wild-type virus and these mutants at the restrictive temperature of 39.5 C, but the mutants were more defective than[r] ...
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Replication Process of the Parvovirus H-1 III. Factors Affecting H-1 RF DNA Synthesis
... Inhibition of RF DNA synthesis by cycloheximide, a-amanitin, and actinomycin D added before HA-DNA synthesisa Drug Time Method of RF added h PI extrac- DNA tion Cycloheximide a-Amanitin [r] ...
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Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA
... If virion populations do contain a minor portion with DNA complementary to the major species, a direct demonstration of this would be possible by separation of the viral strand from its [r] ...
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Analysis of the protein-protein interactions in the parvovirus H-1 capsid.
... The ability to form these high-molecularweight species by treatment of purified empty capsids with PEG suggested that these proteins were derived from VP1 or VP2' or both after the capsi[r] ...
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Replication process of the parvovirus H-1. VI. Characterization of a replication terminus of H-1 replicative-form DNA.
... If the covalent linkage of V to C strand in monomer RF DNA was involved in the initiation of replication of RF DNA or progeny DNA synthesis, then rapidly labeled molecules would be enric[r] ...
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DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.
... We have recently demonstrated the ability of purified polymerase -y to carry out the synthesis in vitro of RF from single-stranded viral DNA 1Sa, a reaction quite similar or identical to[r] ...
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Replication process of the parvovirus H-1. VIII. Partial denaturation mapping and localization of the replication origin of H-1 replicative-form DNA with electron microscopy.
... in the DNAIn addition, this terminus of the A fragment spreading solution; 82% was the formamide concento initiate partial denaturation, hereafter termed the right end of the RF mole- tr[r] ...
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Replication Process of the Parvovirus H-1 I. Kinetics in a Parasynchronous Cell System
... In order to illustrate the kinetics of some of the important events in the viral replication process the following parameters were determined in the same experiment: i the number of cell[r] ...
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