Quantitative real time
Quantitative real-time PCR (qPCR) for Eimeria tenella replication – implications for experimental refinement and animal welfare
... for quantitative tools that can rapidly and accurately determine parasite numbers in both fi eld and experimental ...c quantitative real-time PCR (qPCR) has been applied [18,19], which is a ...
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Rapid Screening of Recombinant Plasmids by Direct Colony Quantitative Real Time PCR
... bacteria in selective medium overnight, preparing plasmid DNA, and digesting with re- striction enzymes. This remains the definitive and often time tested method [2]. How- ever, it can be very tedious and labor ...
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A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... also time consuming and labor intensive. Fluorescence quantitative PCR for adenovirus typing showed acceptable sensitivity, specifi- city, and ...the quantitative real-time PCR (panel ...
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... a quantitative real-time LightCycler PCR was ...a quantitative TaqMan assay, and the bacterial load was compared to the LightCycler ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... Real time-quantitative PCR (RT-qPCR) technology has revolutionized the detection landscape in every area of molecular biology. The fundamental basis of this technology has remained unchanged since ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... of real-time PCR technology has simpli- fied nucleic acid quantification and is coming into more wide- spread ...use. Real-time, quantitative PCR has several advan- tages over older ...
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Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... but Real-time PCR quantification than serological tests is usually recommended because of its sensitivity, reproducibility and broad dynamic range over other ...HBV real-time PCR assay can ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... LightCycler software version 3.3 (Roche Diagnostics) was used to analyze PCR kinetics and calculate quantitative data. A standard curve generated in a separate run was loaded into runs of patient samples (without ...
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A Quick and Efficient Method to Quantify Baculo Virus by Quantitative Real Time PCR
... In this study, it was established that quantitative PCR can be applied for precise and quick quantification of recombinant Baculovirus. It is apparent especially when this method is employed for quantification of ...
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... To validate the reliability of the selected reference genes, the relative expression levels of a key gene Cytochrome P450 ( Cyt P450 - like1 ) involved in betalain biosynthesis were[r] ...
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Altered Expression of Circulating miR-377 and miR-98 in Relapsing-remitting Multiple Sclerosis
... by quantitative real-time polymerase chain reaction (RT-PCR) in peripheral blood mononuclear cells of RRMS patients (recurring patients and two months after ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... culture in Novy-MacNeal-Nicole medium, or inoculation into susceptible animals, such as hamsters (14). However, these methods are rather insensitive and/or time-consuming. The PCR method has proven to be an ...
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Quantification of Substance P mRNA in Human Immune Cells by Real-Time Reverse Transcriptase PCR Assay
... the quantitative real-time PCR and as a template for the synthesis of mRNA in vitro to evaluate the sensitivity of the real-time RT-PCR and to make a standard curve for the ...
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Time-dependent post mortem changes in the composition of intestinal bacteria using real-time quantitative PCR
... Post mortem or even normal changes during life occurring in major gut bacterial populations are not known. We investigated Bacteroides sp., Bifidobacterium sp., Clostridium leptum, Clostridium coccoides, Streptococcus ...
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Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus
... Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, but a lack of standardization and relatively poor precision have hindered its ...of ...
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Detection and survival of SARS in human stool, urine, wastewater and sludge
... Zhai, J., Briese, T., Dai, E., Wang, X., pang, X., Du, Z., Liu, H., Wang, J., Wang, H., Guo, Z., et al. (2004). Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003. Emerging ...
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Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR
... Real-time quantitative PCR. Primers and probes for influenza viruses A and B were selected using Primer Express software (PE Applied Biosystems) and were based on genomic regions highly conserved in ...
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Comparison of Conventional, Nested, and Real Time Quantitative PCR for Diagnosis of Scrub Typhus
... and real-time quantitative PCR (Q-PCR) targeting the ...performed real-time DNA PCR analysis of the human GAPDH gene, along with the ...
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Quantitative Detection of Vibrio cholera Toxin by Real Time and Dynamic Cytotoxicity Monitoring
... the quantitative detection of Vibrio cholerae toxin (CT) in isolates and stool specimens by dynamic monitoring of the full course of CT-mediated cytotoxicity in a real-time cell analysis (RTCA) ...
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Improving and Exploiting Analytical Tools for Real Time Quantitative Studies in Complex Matrices.
... Electrochemical techniques have been used for many years to provide answers to fundamental research questions in challenging environments. For example, coupling fast- scan cyclic voltammetry (FSCV) with carbon-fiber ...
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