Quantitative RT-PCR on candidate genes
Internal control genes for quantitative RT PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages
... six candidate refer- ence genes in osteoblasts, osteoclasts and macrophages and have identified the most suitable set of these genes to use for the accurate normalization of qPCR gene expression data ...
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Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
... Transcription levels of the candidate reference genes in heart, head kidney, spleen, intestine and gills from five fish were calculated 0, 1, 3 and 6 wpc. Data from each sampling ( n = 5) were included in ...
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... identify genes that were the most stably expressed for all of the experimental ...other candidate control genes) and V (the pairwise ...stable genes have the highest M values and are ...
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Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real time quantitative RT PCR (RT qPCR)
... of candidate genes in different experimental subsets/conditions showed in Additional file 4: Table ...the genes tested in this study, using the NormFinder analysis, presented gene stability values ...
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Identification of stable reference genes for quantitative PCR in koalas
... time PCR has been used widely in molecular biology research to evaluate gene expression under certain ...control genes is critical as gene expression should be stable in any experimental conditions or any ...
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Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real time RT PCR
... eight PCR products which confirmed their identity to sequences used for designing primers for the reference ...reference genes using different combinations of GeNorm, BestKeeper and NormFinder can result in ...
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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
... reference genes, reference genes that exhibit stable expression across all assessed samples and/or experimental conditions, we next applied two frequently used statistical algorithms, NormFinder [20] and ...
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Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... using RT-qPCR with SYBR green I detection. The genes included in the study were chosen on the basis of either a previous involvement in antimalarial drug resist- ance or their involvement in parasite ...
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Reference genes for reverse transcription quantitative PCR in canine brain tissue
... reference genes for canine brain tissue. Both genes were tested by Park et ...some candidate control genes were found to be close to each ...the genes ranked second to fifth all have ...
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Identification of Candidate Genes Underlying an Iron Efficiency Quantitative Trait Locus in Soybean
... iron genes located outside the QTL ...18 genes identified within the narrowed introgression were annotated as transcription factors: Glyma03g28440, Glyma03g28500, Glyma03g28610, and Glyma03g28630 (Wang et ...
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
... reference genes are fundamental to get reliable results from ...thirty-nine genes annotated as 18S rRNA (18S ribosomal RNA), CYP (cytochrome), Actin, GAPDH (glyceraldehyde-3-phosphate dehydroge- nase), ...
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Real-time quantitative RT -PCR (Taqman)
... (Advantage RT-for-PCR Kit - Clontech) All reagents listed are provided with the kit a) Quickly thaw each tube in kit and place on ice b) Spin each tube briefly in a tabletop microcentrifuge and return to ...
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CiteSeerX — Review Quantitative RT-PCR: Pitfalls and Potential
... this approach has never been widely used, recent advances in detection methods suggest that this concept has the greatest potential for future quanti- tative RT-PCR development. This ap- proach attempts to ...
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Accurate normalization of real time quantitative RT PCR data by geometric averaging of multiple internal control genes
... control genes are most frequently used to normalize the mRNA ...control genes without proper vali- dation of their presumed stability of ...real-time RT-PCR methods, the requirements for a ...
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Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
... into RT-PCR ...thermostable RT enzyme and gene-specific ...an RT-Taq enzyme blend obviated any need for dilution or aliquoting between the RT and PCR steps of the ...
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Identification of stable reference genes for quantitative PCR in koalas
... Supplementary Figure S1: Agarose gel electrophoresis view of corresponding reference genes (negative contrast ); 100 bp ladder (bioline) used as a DNA marker.. Supplementary Figure S[r] ...
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Amplification Products for PCR and RT-PCR
... Epidermal Growth Factor Receptor, Primer Set Plus Positive Control T59490. ENA-78 Primer Set Plus Positive Control T59500[r] ...
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quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... This method describes a Real-time semi-quantitative TaqMan ® PCR procedure for the determination of the presence of sequence-containing DNA in Rice species DNA extracted from Rice grain samples. For each ...
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DETECTION OF BETA ACTIN AND OSTEOCALCIN GENES EXPRESSION IN DIFFERENTIATED OSTEOBLASTS BY RT PCR
... in PCR, mRNA could be sufficiently isolated and reverse transcribed to cDNA for analysis with PCR methods, Osteocalcin and Beta-actin genes expression, an osteoblast marker gradually increased in ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... Our findings are entirely at variance with results previ- ously described by Kristiansen et al., which led them to assess CD24 as a strong independent predictor of recur- rence not only for Pca but also for lung, breast, ...
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