reference gene
The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells
... stable gene (Figures 3, ...two reference gene set ...PTGS2 gene expression (Figure ...stable reference genes. However, comparison of any of the most stable gene combinations with ...
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Reference gene stability of a synanthropic fly, Chrysomya megacephala
... candidate reference genes for gene expression analysis under various experimental condi- tions in ...the reference evaluation in blowfly ...candidate reference genes ...poor reference ...
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Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real time PCR
... traditional reference genes might not be the optimal choices for quantitating transcript level in rice during RSV- and ...as reference gene for gene ex- pression ...of gene expression ...
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Selection of a suitable reference gene for quantitative gene expression in mouse lymph nodes after vaccination
... suitable reference genes for lymphoid tissues in human, marmo- sets, goats and chickens [4, ...which reference gene would be best suited for analysis of gene expression in the draining lymph ...
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Reference gene selection for gene expression studies using RT qPCR in virus infected planthoppers
... housekeeping gene expression is not always stable and might vary depending on the samples or treatments, it is necessary to first study the stability of several endogenous gene expression in order to select ...
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Reference gene selection for quantitative PCR studies in bovine neutrophils
... trophil reference genes was evaluated using generally accepted Excel-based software tools [24] according to instructions provided by the program developers, ...neutrophil reference genes was performed for ...
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Evaluation of Reference Genes for Differential Gene Expression Study of Bovine Tuberculosis
... a reference gene is used to normalize disparities in RNA recovery and cDNA synthesis ...of gene regulation between samples from within a group, and between samples among different groups [1, 3, ...
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Analysis of sequences regulating larval expression of the Adh gene of Drosophila melanogaster.
... In addition to restoration of normal activity (rela- tive to the AdhF reference gene), the presence of an intact gene on the same plasmid restored appropriate tissue-specific [r] ...
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Transcriptome-based identification of the optimal reference CHO genes for normalisation of qPCR data
... potential reference genes, we analysed two transcriptomic datasets, derived from RNA-seq analyses of ...Ideally reference gene expression levels are similar to those of the gene(s) of ...for ...
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Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China
... ABSTRACT To characterize the diversity and richness and explore the function and structure of swine gut microbiome and resistome in common pig-farming feedlots, we sampled and metagenomic sequenced the feces of pigs from ...
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Variation in stability of endogenous reference genes in fallopian tubes and endometrium from healthy and ectopic pregnant women
... TM reference gene selection kit containing validated lyophilised primer sets for 12 reference genes (PrimerDesign, Southampton, ...of reference genes or 900 nM of NAPE-PLD primers (genbank ...
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Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)
... candidate reference genes were sta- ...overall reference gene but did not exhibit the best per- formance in any single ...stable reference gene using Delta CT, NormFinder, and Best- ...
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Detection of Genomic Alterations Caused by Low-Dose Γ-Irradiation in Human Normal Fibroblast Cells
... even at the hotspots differing from the reference gene. Thus, the ionizing irradiation used in this study did not cause any effects in the 50 genes. However, similar research using human embryonic stem ...
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Validation of a genus-specific gene; TPS, used as internal control in quantitative Real Time PCR of transgenic cotton
... cotton gene to be used an endogenous reference gene for PCR amplification, all gene databanks were investigated for sequences of a low copy reference gene within the genome, to ...
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The expression of mir-126 associated with clinicopathologic features In gastric carcinoma
... However, in another report by Japanese researchers Otsubo T (Otsubo et al., 2011) found that miR-126 through targeted adjustment SOX2 play to the role of the cancer gene. They used the gain and loss of function of ...
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An Atypical Clostridium Strain Related to the Clostridium botulinum Group III Strain Isolated from a Human Blood Culture
... [CTPs] gene, glpK, rpoB, gyrA, and dnaK) (see Table S1 in the supplemental ...in gene analysis (see Table S2 in the supplemental ...to reference 7, were investigated (see Table S1). Gene ...
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The Comparative Toxicogenomics Database facilitates identification and understanding of chemical-gene-disease associations: arsenic as a case study
... To demonstrate the scope and utility of the database for developing hypotheses about chemical-gene/protein-dis- ease networks in humans, we analyzed the CTD arsenic data set as a test case. Arsenic is a global ...
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Felis catus papillomavirus type 2 infection and skin cancer in domestic cats : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand
... FcaPV-2 gene expression in SCCs and the results clearly distinguished two subsets of feline cutaneous ...FcaPV-2 gene expression, suggesting the virus was an incidental ...FcaPV-2 gene expression and ...
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European Reference Network For Rare Vascular Diseases (VASCERN) Outcome Measures For Hereditary Haemorrhagic Telangiectasia (HHT)
... HHT can be diagnosed either clinically using the Curaçao criteria [1] (Table 1), or through a molecular gene test. A patient with definite HHT will have at least 3 of the 4 Curaçao Criteria, or a pathogenic ...
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Phylogenetic analysis of S1 gene of infectious bronchitis virus reveals emergence of new genotype
... This molecular study has revealed that a new genotype can emerge as a result of only few changes in the amino acid composition in the S1 subunit of the virus. The variant presented specific nucleotide and amino acid ...
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