Reverse line blot hybridization assay
Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay
... RLBH assay using a probe-based tech- nique over the phenotypic methods, with sensitivities for de- tection of resistance to INH, RIF, FQs, and second-line ami- noglycosides being 99%, 99%, ...RLBH ...
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Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
... mPCR/RLB assay is less discrimina- tory—although more objective and reproducible—than con- ventional serotyping (2, ...mPCR/RLB assay (3), it can identify 32 serotypes precisely and 58 as belonging to one ...
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Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis
... PCR-reverse line blot (RLB) hybridiza- tion assays for detection and serovar identification of Chlamydia ...RLB assay, labeled omp1 amplicons were hybridized to a membrane containing probes ...
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Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... The complete VP1 genes of 37 isolates, providing one or two examples of each of our commonest 20 serotypes, were sequenced in two fragments by use of published primers (17). These were primer pairs 486-488 and 487-489 ...
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Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay
... Reverse line blot hybridization assays (RLB) have been used for the rapid diagnosis and genotyping of many pathogens. The leishmaniases are caused by a large number of species, and rapid, ...
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Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... The design of primers and probes was based on recently published full cps gene cluster sequences of all 90 pneumococ- cal serotypes (http://www.sanger.ac.uk/Projects/S_pneumoniae /CPS/) and others available in GenBank ...
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Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens
... PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and ...
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Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes
... PCR-based reverse line blot hybridization assay (mPCR/RLB) to detect the genes encoding members of the family of variable surface-localized proteins of Streptococcus agalactiae (group B ...
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Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay
... Fecal samples. All nine samples with uninucleated Entamoe- ba cysts produced the expected amplicon of approximately 550 bp (data not shown). Figure 2A shows the reactivities of these PCR products for the Entamoeba ...
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Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis
... edge it has not been explored for the simultaneous detection of RIF and INH resistance. The lower sensitivity associated with INH resistance is partly due to the fact that phenotypical INH resistance is not fully covered ...
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Replacing Reverse Line Blot Hybridization Spoligotyping of the Mycobacterium tuberculosis Complex
... spoligotyping assay involves the hybridization of amplified sample DNA to nylon membrane-immobilized oligonucleotides whose sequences are representative of 43 spacer ...spoligotyping assay using ...
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Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization
... Reverse line blot hybridization assay. The RLB hybridization assay was mod- ified (31), based on a previously described method ...optimal hybridization temperature ...
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Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low Grade Squamous Intraepithelial Lesion Triage Study
... by reverse line blot hybridization on the ...PCR assay, confirmed the presence of HPV 52 in 63/99 (64%) specimens coinfected with HPV33, -35, or ...
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New Method for Simultaneous Species Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization
... The accurate identification of horse strongyles irrespective of their life cycle stage is of paramount importance in increas- ing the understanding of their biology and epidemiology and the pathogenesis of associated ...
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Simultaneous Detection and Genotyping of “Norwalk Like Viruses” by Oligonucleotide Array in a Reverse Line Blot Hybridization Format
... RLB assay is the size of the RT-PCR product that is ...RLB assay, we were able to detect all and differentiate 94% of the strains in this ...RLB assay for the routine detection and differentiation of ...
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Identification of Ehrlichia spp andBorrelia burgdorferi in Ixodes Ticks in the Baltic Regions of Russia
... and hybridization was performed for 1 h at ...SDS. Hybridization was visualized by incubating the mem- brane with ECL detection liquid (Amersham International plc, Den Bosch, The Netherlands) and exposing ...
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Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
... on reverse line blot and lack of detection on ...the line probe assay have been validated by sequencing the amplified product ...for hybridization with the HPV 40 ...Most ...
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Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay
... mPCR/RLB hybridization assay that permits the reliable, simultaneous detection of 14 known or potential urogenital pathogens, many of which are difficult to identify by other ...
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Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single Hybridization, Reverse Line Blot Detection Method
... probe hybridization has been described elsewhere (4, 21), and a schematic of the procedure is presented in ...ink line, detecting 27 individual HPV genotypes and two concentrations of the b-globin control ...
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Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay
... amino linker extremity were carried out as previously described (7). Positive controls were plasmids containing the 23S-5S intergenic spacer sequence from R. prowazekii, R. conorii, and R. aeschlimannii, kindly provided ...
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