RNA high throughput sequencing analysis
Analysis of the Aspergillus nidulans transcriptome using high-throughput RNA sequencing
... A high level of transcription has been observed, with approximately ...to RNA-seq reads, indicating broad transcriptional utilisation of the ...this analysis did not take into account regions such as ...
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The RNA workbench: best practices for RNA and high-throughput sequencing bioinformatics in Galaxy
... reproducible analysis platform For the fast dissemination of the RNA workbench, as well as for an easy integration with other HTS analysis tasks, we implemented the RNA workbench within the ...
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The RNA workbench: Best practices for RNA and high-throughput sequencing bioinformatics in Galaxy
... reproducible analysis platform For the fast dissemination of the RNA workbench, as well as for an easy integration with other HTS analysis tasks, we implemented the RNA workbench within the ...
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Genome-wide transcriptome analysis in murine neural retina using high-throughput RNA sequencing
... with high- est expression in both replicates were three retinal disease genes: rho- dopsin (Rho) ...Statistical analysis indicates clearly that the expression level of disease genes is signi ficantly higher ...
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Analysis of Paramyxovirus Transcription and Replication by High-Throughput Sequencing
... total RNA at 6 h ...total RNA at 6 h ...total RNA at 18 and 24 h ...total RNA, after which the abundance decreased to 2 to 3% by 24 h ...
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Analysis of paramyxovirus transcription and replication by high throughput sequencing
... total RNA at 6 h ...total RNA at 6 h ...total RNA at 18 and 24 h ...total RNA, after which the abundance decreased to 2 to 3% by 24 h ...
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Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
... good RNA-Seq library should have a narrow size range, retain strand information, and contain little RNA contamination from other species and few inserts from ribosomal ...Since high through- put ...
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Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis.
... examine high- throughput sequencing experiments are those designed to examine RNA-seq experiments [1], and these tools are often advocated for use in other experimental designs that generate ...
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Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis
... many high-throughput sequencing stud- ies is to identify those genes or features that make a significant difference between two or more ...include RNA sequencing (RNA-seq), ...
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Analysis options for high throughput sequencing in miRNA expression profiling
... them challenge the quality of qPCR in miRNA expression measurements [12], other show good qPCR – microarray [13-15] or HTS – microarray [16-19] concordance. Apart from other studies our work for the first time compares ...
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Applications of High-Throughput Sequencing Data Analysis in Transcriptional Studies
... and RNA-protein interactions, researchers have designed multiple deep learning models and obtained promising ...by high-throughput technologies and predict the binding targets ...the RNA ...
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Characterizing alternative splicing and long non-coding RNA with high-throughput sequencing technology
... 2.4 Methods 2.4.1 Preparation of Mouse BMSCs A single-step stem cell purification method was employed as previously described [123]. Briefly, BMSCs were collected from the bilateral femurs and tibias of sacrificed mice ...
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Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing
... the RNA editome in pigs based on strand-specific RNA sequencing data and whole-genome sequencing data of the brain, fat, heart, liver, lung, muscle and ovary from three 180-day-old Large White ...
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High throughput RNA interference screening using pooled shRNA libraries and next generation sequencing
... data analysis Raw images from the Illumina GAIIx were processed using the GApipeline (version ...or sequencing errors could align in different regions to unaltered reads, again giving a false picture of the ...
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BRB seq: ultra affordable high throughput transcriptomics enabled by bulk RNA barcoding and sequencing
... use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis ...lower RNA quality and being up to 25 times ...
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High-Throughput Sequencing of RNA Silencing-Associated Small RNAs in Olive (Olea europaea L.)
... cleavage analysis might be false positive ...RACE analysis as a cleavable target, although the miRNA or tasiRNA responsible for cleavage could not be Figure ...
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Telomere and Proximal Sequence Analysis Using High-Throughput Sequencing Reads
... non-coding RNA, and to better characterize their transcription start ...ChIP-seq analysis in the human subtelomere found colocalization of CTCF and Cohesin directly adjacent to the telomere and throughout ...
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Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS)
... and RNA-Seq tell a consistent story? ”The relationship is not quite linear … but the vast majority of the expression values are similar between the ...Similarly, RNA- Seq is a sampling method and stochastic ...
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Universal Count Correction for High-Throughput Sequencing
... DEseq analysis pipeline, we used replicates to estimate exon-level overdispersion and identified exons differential across replicates at ...23 RNA-seq experiments and 87 ChIP-seq ...
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Comprehensive High-Throughput RNA Sequencing Analysis Reveals Contamination of Multiple Nasopharyngeal Carcinoma Cell Lines with HeLa Cell Genomes
... specific RNA-seq data set was analyzed by extracting the first 25- mer reads from each end of the 100-base read and aligning them independently of each other to a reference genome containing both the human hg19 ...
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