Single Cell
Single cell sequencing in stem cell biology
... of single-cell omic ...the single-cell omic datasets, such as high technical noise and high false-negative rates, and the pros of these data- sets, such as high sampling numbers and UMIs or ...
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Challenges and emerging directions in single cell analysis
... from single-cell studies have enabled the devel- opment of mathematical models that represent the distribution of cell states as one sampled from a dynamic system [11, 17, 19, ...view, cell ...
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Mapping chromatin modifications at the single cell level
... amplification, cell- specific barcodes are ...modification. Single cells are sorted into tubes and calcium is added to trigger MNase-mediated ...enables cell-specific barcode incorporation. (F) ...
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Multiplexed, targeted profiling of single cell proteomes and transcriptomes in a single reaction
... high cell-cell variance – this could reflect correlated activation of RNA and protein in only a subset of cells (bimodality), echoing previous findings in induced systems ...for single-cell ...
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Cell fixation and preservation for droplet-based single-cell transcriptomics
... study. Single-cell sequencing data from individual runs of primary tissues from both organisms yielded highly reproducible ...per cell) to our mouse brain sample, the single-cell ...
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Single cell analysis of endothelial morphogenesis in vivo
... at single-cell resolution have hampered study of the cell biology of ECs in ...or cell hollowing involving fusion of intracellular vacuoles have both been proposed as mechanisms for lumen ...
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Single-cell morphology encodes metastatic potential
... 1. Cell polymorphism—cell morphology is a highly heritable trait at the single-cell ...establish single-cell clones (SCCs) from a parental cell ...quantify cell ...
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Towards in situ Single Cell Systems Biology
... Bates et al, with Glucose Oxidase as the oxygen scavenger and BME as the reducing agent. Analysis. Images from diffraction limited and super-resolution experiments were analyzed with a custom written Mathematica script, ...
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Single-cell RNA-sequencing of the brain
... To quantify gene expression, sequencing reads from high quality cells are aligned to a reference genome and gene counts are computed. If UMIs were used, transcript molecules may be counted directly since the number of ...
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Single-Cell Epigenomics In Cancer Research
... first single-cell methylome analysis was reported in 2013 ...a single tube prior to bisulfite conversion, minimizing DNA losses arising from multiple purification ...at single-base resolution ...
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The potential of single cell profiling in plants
... that single-cell profiles from highly localized plant quiescent center (QC) cells are relatively dispersed in the first two axes of a PCA ...in single-cell studies [17, 33, ...plant ...
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Cell adhesion manipulation through single cell assembly for characterization of initial cell-to-cell interaction
... regulate the adhesion, homing, and mobilization of leukemia cells [26–28]. Disruption of these interactions by SDF-1/CXCR4 antagonists represents a novel strategy for target- ing leukemia/bone marrow microenvironment ...
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Single-cell analysis of glandular T cell receptors in Sjögren’s syndrome
... T cell development, the rearrangement of TCRβ chains is tight- ly regulated to ensure production of a single TCRβ chain in each T ...a single cell (13, ...
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A two component red blood cell model for single cell mechanic
... employ single cell approach overcome several problems faced by the former ...on single cell mechanical properties measurements can be found from (Ahmad & Ahmad 2014) ...
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Quantification of cell identity from single cell gene expression profiles
... 15 cell types queried; Figure 4C), indicating a quanti- fiable loss or decrease in cell ...these single-cell profiles and previous work that the cells selected were not in the distal end of ...
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Single Cell Isolation Using Optical Tweezers
... 1064-nm wavelength optical trapping of Escherichia coli cells using on-chip single cell cultivation system. Quantum Dot-Based Thermal Spectroscopy and Imaging of Optically Trapped 628[r] ...
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Comparative Analysis of Different Single Cell Metamaterial
... mesh cell. We also present a comparative analysis on tunability of single cell metamaterial structure to a different frequency band of ...restricted cell size of ...mesh cell ...
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Single cell states in the estrogen response of breast cancer cell lines
... model cell transition rates and the corresponding population dynamics, ...the single-cell events involved in the oestrogen response of the MCF-7 cells, and the resulting population ...MCF-7 ...
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Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
... A. Dilution experiment summary. See section 3.5.1 for detailed information. B. Single cell amplification methods used. Protocols involve two key steps: conversion of RNA (blue) to cDNA (green), and ...
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Automated cell cycle and cell size measurements for single cell gene expression studies
... A. Single mammalian cells compensate for differences in cellular volume and dna copy number through independent global transcriptional ...of cell‑to‑ cell heterogeneity in single‑cell ...
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