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single-chain Fv

Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT

Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT

... Construction and purification of hDM- α H-C6.5 MH3B1 We have previously shown that hPNP with the two muta- tions Glu201Gln:Asn243Asp, unlike wild-type hPNP, converts a relatively non-toxic prodrug, F-dAdo to the ...

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High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

... We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia ...

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Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo

Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo

... One of the PRRs present in DCs is DEC-205 (or CD-205) which is a C-type lectin receptor involved in recognition of ligands expressed during apoptosis and necrosis of cells [24], in recognition of CpG oligonucleo- tides ...

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Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

... lymphoma cell line Ramos, resulting in 100% disease-free survivors at 300 days [6]. Several first generation anti- CD22 ITs have been described in the past some chemically conjugated to plant deglycosylated ricin ...

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GPI anchored single chain Fv   an effective way to capture transiently exposed neutralization epitopes on HIV 1 envelope spike

GPI anchored single chain Fv an effective way to capture transiently exposed neutralization epitopes on HIV 1 envelope spike

... Importantly, the expression of GPI-scFv X5 on the surface of human CD4+ T cells confers long-term resistance to HIV-1 infection, HIV-1 envelope-mediated cell-cell fusion and the infectio[r] ...

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Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

... The aim of this study was to utilize DoE in order to investigate the effect of pH, temperature and MeOH concentration on production of the anti-keratin 8 sin- gle-chain Fv, TS1-218, by P. pastoris strain ...

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Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

... As shown in Figure 5B, the βgal activity present in the mutants was about five to seven times higher than in the strain expressing the wild type 225.28S scFv fused to the α-fragment and [r] ...

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Single-Chain Fv-Based Anti-HIV Proteins: Potential and Limitations

Single-Chain Fv-Based Anti-HIV Proteins: Potential and Limitations

... a single promoter with a 2A self-processing peptide inserted between the anti- body heavy and light chains permitted ⬃1-mg/ml IgG levels to be achieved with AAV-transduced liver expression ...a ...

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Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory

... Results: We have generated new broad host range scFv expression constructs and assessed their production in the Pseudomonas putida KT2440 host. Two scFvs bind either to human C-reactive protein or to mucin1, proteins of ...

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Paracrine Inhibition of Prion Propagation by Anti-PrP Single-Chain Fv Miniantibodies

Paracrine Inhibition of Prion Propagation by Anti-PrP Single-Chain Fv Miniantibodies

... The bovine spongiform encephalopathy epidemic (42) can be arguably deemed the direct consequence of flawed technol- ogy assessment and is caused by prions that were amplified through the bovine food chain (41). ...

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Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus

Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus

... West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent or treat disease. Passive immunization with antibodies against the envelope protein ...

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MicroRNA expression profiles in human CD3+ T cells following stimulation with anti human CD3 antibodies

MicroRNA expression profiles in human CD3+ T cells following stimulation with anti human CD3 antibodies

... Abbreviations FvFc: single chain Fv fragment fused to a human IgG1 Fc; mAb: monoclonal antibody; miRNAs: microRNAs; PBMC: peripheral blood mononuclear cells; qPCR: quantitative PCR; TCR:[r] ...

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Antibody mediated delivery of IL 10 inhibits the progression of established collagen induced arthritis

Antibody mediated delivery of IL 10 inhibits the progression of established collagen induced arthritis

... fragments single-chain Fv(L19) and sin- gle-chain Fv(HyHEL10), preceded by a signal sequence required for secretion of recombinant proteins, were geneti- cally fused to human IL-10 with ...

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The framework of image recognition based on modified freeman chain code

The framework of image recognition based on modified freeman chain code

... Result on two line drawings tested on the framework is presented in this section. The discussion on input and output of four steps in the framework of first line drawing (LD1) namely pre- processing, corner detection, ...

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The effectiveness of asking behaviors among 9–11 year-old children in increasing home availability and children’s intake of fruit and vegetables: results from the Squire’s Quest II self-regulation game intervention

The effectiveness of asking behaviors among 9–11 year-old children in increasing home availability and children’s intake of fruit and vegetables: results from the Squire’s Quest II self-regulation game intervention

... home FV availability and child FV intake have shown significant [56], but also non-significant predictive associations [57, ...in FV home availability and child FV intake, as can be analyzed ...

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ENDLESSLY SINGLE-MODE HIGHLY POLARIZATION MAINTAINING BIREFRINGENT PHOTONIC CRYSTAL FIBER WITH ZERO DISPERSION AT WAVELENGTH 1.55µm

ENDLESSLY SINGLE-MODE HIGHLY POLARIZATION MAINTAINING BIREFRINGENT PHOTONIC CRYSTAL FIBER WITH ZERO DISPERSION AT WAVELENGTH 1.55µm

... endlessly single-mode highly polarization maintaining birefringent photonic crystal fiber at wavelength ...Λ=2.3µm,endlessly single- mode ( V eff ≤ л ) , high birefringence ...

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The Spatial Relativity and Its Physical Consequences

The Spatial Relativity and Its Physical Consequences

... As presented above, BBM maintains the existence of the causally disconnected regions in cosmic space, and this is clearly contrary to the spirit of physical unification. Here, we show that, the difficulties can be ...

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Analysis of wild-derived mice for Fv-1 and Fv-2 murine leukemia virus restriction loci: a novel wild mouse Fv-1 allele responsible for lack of host range restriction.

Analysis of wild-derived mice for Fv-1 and Fv-2 murine leukemia virus restriction loci: a novel wild mouse Fv-1 allele responsible for lack of host range restriction.

... None of the wild mice showed the Fv-JT or Fv-1b restriction patterns characteristic of most inbred strains, several mice resembled the few inbred strains carrying Fv-1n1, and most differ[r] ...

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Production of single chain Fab (scFab) fragments in Bacillus megaterium

Production of single chain Fab (scFab) fragments in Bacillus megaterium

... and Fab are produced in bacteria. The scFv format is com- posed of the variable regions of the antibody heavy (VH) and the light chain (VL) linked by a 15–25 amino acid linker [4,5]. ScFvs have the tendency to ...

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Suppression of Established Friend Virus Leukemia by Statolon

Suppression of Established Friend Virus Leukemia by Statolon

... Demonstration of a Latent Infection in Clinically Normal Mice Received for publication 4 March 1969 A single inoculation of statolon into mice with established Friend virus FV leukemia c[r] ...

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