single specific primer PCR
Differentiation of Campylobacter jejuniSerotype O19 Strains from Non O19 Strains by PCR
... PCR specific for O19 or non-O19 strains. Because this single nucleotide difference at the downstream D14307 binding re- gion in gyrB discriminated between O19 and non-O19 strains, we adopted a ...
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AlignMiner: a Web-based tool for detection of divergent regions in multiple sequence alignments of conserved sequences
... conserved primer candidates from MSAs [19,20], for comparative studies of genes and genomes ...(beyond single nucleotide dissimilarities) that would provide a numerical score related to divergence signifi- ...
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Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene
... the single nucleotide polymorphism (SNPs) rs972283 (A/G) of KLF14 gene with in a global ...T-ARMS PCR assay to genotype ...two specific primer pair for T-ARMS ...T-ARMS PCR were con- ...
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Detection and Typing of Human Papillomavirus by E6 Nested Multiplex PCR
... ternatively, PCR assays utilizing consensus or general primers, ...a single reaction (10, 12, 20, ...nested PCR assay has been shown to increase the overall sensitivity com- pared to that of each ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... the PCR efficiency among the different strains were equivalent to the single primer-probe ...as PCR-microarray, PCR-sequencing and PCR-fragment length analysis have been ...
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A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
... the primer corresponded to the 3'-end of the DNA frag- ment to be ...the PCR amplifications from the entry clones were universal prim- ers matching the donor vector sequences outside of the attL1/attL2 ...
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Bead based assays to simultaneously detect multiple human inherited blood disorders associated with malaria
... Genomic PCR, B. ASPE (allele specific primer extension) and ...a single copy of the G6PD gene), above 75% wild type MFI signifies that the sample is wild type and below 25% wild type MFI ...
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Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs
... Helicobacter pylori is a microaerophilic Gram-negative bacterium that chronically infects the gastric mucosa. It is recognised as a human pathogen associated not only with chronic gastritis [1], but also with peptic ...
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LSSP PCR of Trypanosoma cruzi: how the single primer sequence affects the kDNA signature
... simple, PCR-based tech- nique that permits the detection of single or multiple mutations in gene-sized DNA ...to PCR using high con- centrations of a single specific oligonucleotide ...
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An improved allele-specific PCR primer design method for SNP marker analysis and its application
... Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR ...
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Species Specific DNA Profiling Mycobacterial Genomes Using Polymerase Chain Reaction with Single Universal Primer (UP-PCR)
... apply PCR technique for discriminating tuberculosis and mycobacteriosis [19- ...conventional PCR and PCR "fingerprinting", namely, the species-specific DNA product and one universal ...
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... primed PCR (AP-PCR) [8], random amplified polymorphic DNA (RAPD) [9,10], repetitive sequence PCR (rep-PCR) [11], restriction analysis of the mitochondrial DNA [12,13], semi-nested PCR ...
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Development of nested polymerase chain reaction detection of Mycosphaerella spp and its application to the study of leaf disease in Eucalyptus plantations
... (9,11,52). PCR-based methods exploit genetic sequence differences to provide early, rapid, specific, and sensitive detection and identification of pathogens of concern regardless of the presence of ...
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Detection of Cryptdin in Mouse Skin
... The PCR mixture was overlaid with mineral ...of PCR were analyzed by gel electrophoresis on 2% agarose ...by PCR of cryptdin from cDNA was accomplished with primers Defcr p130 ...
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Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts.
... Total RNA from these cells was analyzed by reverse transcription and PCR amplification by using the following specific combinations of oligonucleotide primers: LMP1 mRNA, 5' 2.8 primer a[r] ...
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Characterisation of heavy metal and antibiotic-resistance genes in oral bacteria
... a single stranded nick at a specific origin in the plasmid by an initiator ...a single replication cycle from the template strand, the displaced single stranded (SS) non-template strand is ...
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Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures
... two PCR typing methods am- plifying the flanking sequences on both sides of IS6110, IS6110-PCR and IS6110-inverse-PCR, and we compare the results with the well-standardized RFLP typing ...of ...
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Simian immunodeficiency virus displays complex patterns of RNA splicing.
... Colonies representing the PCR amplification products of the primer pairs PCRAR-PCR25 and PCRSTATPCR25 were initially screened by hybridization with oligonucleotide probes specific for sp[r] ...
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Development and Validation of a Multiplex PCR for Detection of Scedosporium spp in Respiratory Tract Specimens from Patients with Cystic Fibrosis
... multiplex PCR assay for the detection and identification of the three major clinically important Scedosporium species and validated using sputum specimens collected from patients seen at a major Australian cystic ...
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Culture Independent Identification of Periodontitis Associated Porphyromonas and Tannerella Populations by Targeted Molecular Analysis
... PCR with primer pair 27F-PT997R gave amplification prod- ucts of the expected sizes for all of the reference Porphyromo- nas and Tannerella strains tested. A total of 308 cloned 16S rRNA genes were ...
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