small T antigen
Genomic structure of human polyoma virus JC: nucleotide sequence of the region containing replication origin and small-T-antigen gene.
... Apparently, the similarity of the sequences for replication origin and of the amino acid sequences for putative small T antigen between JCV and other primate polyoma viruses proves that [r] ...
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Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... large T and p53 for SV40-mediated cellular transformation is firmly estab- lished (16, 23, 52, 56, 59, 73, 74), although the functional consequences of this interaction are not fully understood and are still a ...
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Isolation and characterization of NIH 3T3 cells expressing polyomavirus small T antigen.
... DISCUSSION By introducing the small T-antigen gene into cells with a retrovirus vector as replication-defective retrovirus, we were able to isolate NIH 3T3 cells expressing only polyomav[r] ...
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Mutational analysis of polyomavirus small-T-antigen functions in productive infection and in transformation.
... This result shows that mutant forms of small T antigen that are defective in the stimulation of viral DNA synthesis and virion assembly during productive infection are still active in th[r] ...
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Induction of p53-Independent Apoptosis by Simian Virus 40 Small t Antigen
... Simian virus 40 small t antigen (st) is required for optimal transformation and replication properties of the virus. We find that in certain cell types, such as the human osteosarcoma cell line U2OS, ...
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Simian virus 40 small t antigen trans activates the adenovirus E2A promoter by using mechanisms distinct from those used by adenovirus E1A.
... To determine whether small t antigen employs a mechanism different from that domain m, or both, I compared the effects of transfecting plasmids expressing encoded in ElA domain small t a[r] ...
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Polyomavirus Small t Antigen Interacts with Yes-Associated Protein To Regulate Cell Survival and Differentiation
... polyomavirus small t antigen (PyST) regulates cell cycle, cell survival, apoptosis, and differentiation and cooperates with middle T antigen (MT) to transform primary cells in vitro and ...
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Localization of the simian virus 40 small t antigen in the nucleus and cytoplasm of monkey and mouse cells.
... 623 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Monkey and mouse cells producing simian virus 40 small t antigen in the absence of clearly detectable intact or trun[r] ...
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Properties of simian virus 40 small t antigen overproduced in bacteria.
... To detect SV40-related proteins expressed in bacteria and compare them with authentic SV40 small t antigen, both SV40-infected monkey cells and HP1 bacterial cells were pulse-labeled wit[r] ...
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Merkel Cell Polyomavirus Small T Antigen Targets the NEMO Adaptor Protein To Disrupt Inflammatory Signaling
... Merkel cell carcinoma (MCC) is a highly aggressive nonmelanoma skin cancer arising from epidermal mechanoreceptor Merkel cells. In 2008, a novel human polyomavirus, Merkel cell polyomavirus (MCPyV), was identified and is ...
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Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.
... Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen i[r] ...
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A novel simian virus 40 early-region domain mediates transactivation of the cyclin A promoter by small-t antigen and is required for transformation in small-t antigen-dependent assays.
... (SV40) small-t antigen (small-t) en- hances the ability of SV40 to transform several growth-ar- rested cell types in vitro and to induce tumors in vivo, espe- cially in nonreplicating ...
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Simian virus 40 small-t antigen stimulates viral DNA replication in permissive monkey cells.
... To explore this apparent paradox between in vivo and in vitro experiments, we examined the effect of small t on replication of microinjected viral DNA and the effects of small-t antigen [r] ...
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Mutational analysis of simian virus 40 small-t antigen.
... 2895 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Several point mutations in the simian virus 40 SV40 small-t antigen have been analyzed for their effects on protein[r] ...
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Comparisons between Murine Polyomavirus and Simian Virus 40 Show Significant Differences in Small T Antigen Function
... SV40 small T antigens promote cel- lular DNA ...SV40 small T antigens are known to promote cellular DNA replication (15, ...two small T antigens directly for their ability to ...
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Expression of the gene for the polyoma small T antigen in Escherichia coli.
... The DNA sequence consisting of the lac control element joined to the coding region for polyoma small T antigen in the manner described could potentially express a fused protein consistin[r] ...
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Merkel cell polyomavirus small T antigen mediates microtubule destabilization to promote cell motility and migration
... Similar assays were also performed using wild-type or trans- FIG 5 Microtubule destabilization is required for MCPyV ST-mediated cell motility. (A) MCC13 cells expressing EGFP or EGFP-ST were incubated in the presence of ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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Restricted Protein Phosphatase 2A Targeting by Merkel Cell Polyomavirus Small T Antigen
... large T (LT) and sT early proteins that are required for MCV-positive Merkel cell carcinoma (MCC) cell survival and proliferation (38, ...virus small T ...large T stabilization domain (LSD) ...
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Transrepression of RNA polymerase II promoters by the simian virus 40 small t antigen.
... A plasmid encoding intact t was cotransfected into CV-1P cells together with a plasmid, pFC700, which contains a copy of the human c-fos promoter nt -710 to +42 relative to the start sit[r] ...
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