SV40(II)DNA
An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... shift DNA-binding experiments showed a reduction in the site II binding activity of T83-708 ...origin DNA–T83-708 complexes ap- peared to contain more than 12 monomers ...origin DNA binding, ...
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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... Purification of GST fusion proteins. GST-TBP and GST-T antigen fusion proteins were expressed and purified as described previously (70), with the following modifications. An overnight culture of E. coli BL21(pLysS), ...
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...(SV40) DNA has served as a useful model system for studying ...
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Study of SV40 large T antigen nucleotide specificity for DNA unwinding
... viral DNA replication in mammalian ...the SV40 origin to initiate genomic ...unwind DNA, other NTPs can also support low DNA helicase ...
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Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.
... 5 0022-538X/88/051598-08$02.00/0 Copyright © 1988, American Society for Microbiology Mutations in the Phosphorylation Sites of Simian Virus 40 SV40 T Antigen Alter Its Origin DNA-Binding[r] ...
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Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
... We previously reported 28 that only a very small fraction of T Ag binds with a higher salt-sensitive affinity to SV40 DNA than to calf thymus DNA-cellulose under these conditions and tha[r] ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ...
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ...
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An Okazaki piece of simian virus 40 may be synthesized by ligation of shorter precursor chains.
... These data led us to propose that the synthesis of an SV40 Okazaki piece could be itself discontinuous and could comprise the following steps: i iRNA synthesis by DNA primase, ii iRNA ex[r] ...
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cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Binding of[r] ...
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Mechanisms of interference with simian virus 40 (SV40) DNA replication by trans-dominant mutants of SV40 large T antigen.
... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ...
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Simian Virus 40 Deoxyribonucleic Acid Transcription In Vitro: Binding and Transcription Patterns with a Mammalian Ribonucleic Acid Polymerase
... It was found that i the majority of supercoiled SV40 DNA molecules displayed a single binding site for the enzyme, ii the supercoiled structure of SV40 DNA was frequently retained during[r] ...
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Structural topography of simian virus 40 DNA replication.
... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ...
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Structural requirements for simian virus 40 replication and virion maturation.
... Ongoing viral DNA replication panels d and e led to a continuous increase in nuclear matrix-associated SV40 minichromosomes, but a significantly higher proportion of SV40 minichromosomes[r] ...
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Structural changes in simian virus 40 chromatin as probed by restriction endonucleases.
... 11 shows that the initial linear DNA III was eluted from the gels after cleavage of the SV40 chromatin by HpaI was digestion of SV40 chromatin by HpaI, and the not at a unique site, for [r] ...
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Use of Nondefective Adenovirus-Simian Virus 40 Hybrids for Mapping the Simian Virus 40 Genome
... The order of these early SV40 antigen inducing regions in the SV40 DNA segments contained in the nondefective hybrids is: U antigen, tumor specific transplantation antigen, and T antigen[r] ...
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...
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Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
... Solid lines, pBR322 sequences; open bars, SV40 or Adl2 sequences; 0, origin of SV40 DNA replication; shaded regions, SV40 sequences containing the 5' striped regions and 3' crosshatched [r] ...
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Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.
... Lanes: a and i, SV40 tsA58 DNA, form I; b and j, SV40 tsA58 DNA cleaved with HaeII, form III; c, intracellular viral DNA from SV-AdElB-B-infected cells cleaved with XbaI; d, intracellula[r] ...
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