[PDF] Top 20 cis-acting elements in the lytic origin of DNA replication of Epstein-Barr virus.

... The efficiency of lytic-cycle DNA replication is influenced by sequence elements flanking the core of the lytic origin in herpes simplex virus 41.. Like oriLyt of EBV, these flanking seq[r] ... See full document

... oriLyt-mediated DNA replication, we analyzed newly replicated p526 DNA by Southern blot ...cellular DNA was extracted from D98HR1 cells cotransfected with pCMV-BZLF1 and the ...The DNA ... See full document

... the lytic phase of EBV’s life cycle had been induced with a combination of TPA and ...protein DNA contact points (see ...the DNA-protein complexes in this assay by comparing uninduced and induced ... See full document

... the virus ending at an AATAAA poly(A) signal sequence (28, ...EBV lytic origin (OriLyt R ) with an identical promoter, was also identified ...during lytic replication (20, 30, ...EBV ... See full document

... response elements in some late pro- moters (17), ZEBRA likely stimulates the promoters’ activities through an indirect mechanism which requires other viral ac- tivities (compare ...be lytic DNA ... See full document

... sequence elements of EBNA1: the loop- ing domain, the acidic tail, the proline loop, and the WF ...the DNA replication function, indicating that the looping domain plays an essential role in ...the ... See full document

... Unlike the interaction of bEBNA1 with onP, in which one element FR is bound prior to interaction Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest a bEBNAl proteins 1.4 pm[r] ... See full document

... EBV DNA and EBV-specific anti-VCA and anti-EA antibodies in the neurological stage but not during convales- cence ...direct virus invasion of the central nervous system, although the pathogenesis is not ... See full document

... the Epstein-Barr virus (EBV) oriP sequence stably transfected into Akata Burkitt’s lymphoma cells were used to analyze EBV lytic cycle gene ...late lytic cycle promoters revealed that ... See full document

... imperfect DNA hairpin formed within the top strand of a critical element within the lytic origin ...this cis-acting component of OriLyt, the UEE, contains two adja- cent, inverted Zta ... See full document

... primary replication-specific function for Zta in the infected cell since the tripartite helicase-primase complex is itself capable of nuclear ...of replication postulate that the initial steps in DNA ... See full document

... KSHV lytic replication, but its mechanism of action is also unknown (47, ...viral lytic replication, the mechanisms govern- ing the cysteine-dependent regulation through C189 have ap- parently ... See full document

... the lytic replication program in the absence of any other external stimuli, allowing us to analyze precisely the architec- ture of the replication compartments of EBV lytic ...EBV ... See full document

... short-term replication assays were also used to characterize mutated oriP ...plasmid DNA from transfected-cell populations (10) or compared the amount of replicated plasmid DNA to the total amount of ... See full document

... described elements in the P1 pro- moter, these elements will be now be referred to as ZIVA (site I) and ZIVB (site ...ZIVB elements, YY1 purified from HeLa cells by DNA affinity chromatography ... See full document

... the origin of lytic ...response elements (ZREs) of Rp (38, ...EBV replication protein, in several subsequent experiments (see ...an origin binding ... See full document

... line B95-8 (24, 25). Lytic replication was induced by treatment with 20 ng of tetradecanoyl phorbol acetate (TPA) per ml for 3 days. Fixed- or live-cell preparations were then incubated with monoclonal ... See full document

... Not only is there a high degree of sequence homology in HVP ori-Lyt, but the overall organization of the origin is conserved, with functional promoter and enhancer domains Downloaded fro[r] ... See full document

... Thus, the findings that there is no inverted repeat within 10 min of lambda exonuclease digestion, the size 10 x 106 daltons of the terminus of the long of most EBV DNA molecules was bet[r] ... See full document

... single-stranded DNA-binding protein BALF2 has also been found to contact the BALF5-BMRF1 proteins ...core replication proteins appear to be tethered by interactions to one or more of these three ...the ... See full document