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[PDF] Top 20 Analysis options for high throughput sequencing in miRNA expression profiling

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Analysis options for high throughput sequencing in miRNA expression profiling

Analysis options for high throughput sequencing in miRNA expression profiling

... miRNA expression profiling guidelines In a summary of the results we propose a guidance for miRNA expression profiling ...differential expression measurements we propose ... See full document

12

Original Article Detection of miRNA differential expression of necrotizing enterocolitis in newborns by high-throughput sequencing

Original Article Detection of miRNA differential expression of necrotizing enterocolitis in newborns by high-throughput sequencing

... Bioinformatics analysis over 126 differentially expressed miRNAs indicates that the enriched GO terms were immune response, chemotaxis- related regulation, cell junction, T-cell activa- tion, regulation of ... See full document

9

Protein expression profiling arrays: tools for the multiplexed high-throughput analysis of proteins

Protein expression profiling arrays: tools for the multiplexed high-throughput analysis of proteins

... A wide variety of surface substrates and attachment chem- istries have been evaluated for the immobilization of cap- ture agents on protein microarrays. They fall into two basic categories. The simplest way to immobilize ... See full document

7

High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling

High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling

... the high levels of virus RNA synthesised in infected cells at late time ...ribosome profiling, data from mock- infected samples and tests for contamination are often not reported, so the level of contamina- ... See full document

44

High-throughput Sequencing and Profiling of Antibody Repertoire and the Promises for Diagnostic and Therapeutic Applications

High-throughput Sequencing and Profiling of Antibody Repertoire and the Promises for Diagnostic and Therapeutic Applications

... ABSTRACT High-throughput DNA sequencing technologies are advancing at rapid state and transforming our indulgent about humoral immune ...from high-throughput DNA sequencing of ... See full document

11

Analysis of paramyxovirus transcription and replication by high throughput sequencing

Analysis of paramyxovirus transcription and replication by high throughput sequencing

... protein expression can be further controlled by the generation of read- through mRNAs, as downstream genes transcribed as polycistronic mRNAs would not FIG 8 Theoretical mRNA abundance gradients compared to actual ... See full document

17

Analysis of Paramyxovirus Transcription and Replication by High-Throughput Sequencing

Analysis of Paramyxovirus Transcription and Replication by High-Throughput Sequencing

... protein expression can be further controlled by the generation of read- through mRNAs, as downstream genes transcribed as polycistronic mRNAs would not FIG 8 Theoretical mRNA abundance gradients compared to actual ... See full document

17

Next-generation sequencing analysis of miRNA expression in control and FSHD myogenesis

Next-generation sequencing analysis of miRNA expression in control and FSHD myogenesis

... that miRNA sequences can regulate skeletal myogenesis by controlling the process of myoblast proliferation and ...deep analysis of miRNA expression in control and FSHD myoblasts during ... See full document

14

Analysis of HIV-1 Expression Level and Sense of Transcription by High-Throughput Sequencing of the Infected Cell

Analysis of HIV-1 Expression Level and Sense of Transcription by High-Throughput Sequencing of the Infected Cell

... way analysis identified “cellular growth and proliferation” and “RNA posttranscriptional modification” as prominent modu- lated functions, and it indicated that networks related to cell cycle, DNA replication and ... See full document

7

Regulation expression pathway analysis (REPA): a novel method to facilitate biological interpretation of high throughput expression profiling data

Regulation expression pathway analysis (REPA): a novel method to facilitate biological interpretation of high throughput expression profiling data

... literature analysis on 50 randomly selected ...this analysis, we focused only on predictions that associate transcription factors to well established manually curated gene sets from sources such as KEGG, ... See full document

128

Applications of High-Throughput Sequencing Data Analysis in Transcriptional Studies

Applications of High-Throughput Sequencing Data Analysis in Transcriptional Studies

... computational analysis pipeline is depicted in Figure ...differential analysis, the RNA-Seq read counts will be normalized to eliminate the variations resulting from different library ...the ... See full document

97

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing

... the throughput, which actually allowed an analysis of 31 libraries in three eighth of the capacity of a single Illumina GAII sequencing run (Table ...single sequencing run by simply employing ... See full document

8

Viral expression associated with gastrointestinal adenocarcinomas in TCGA high-throughput sequencing data

Viral expression associated with gastrointestinal adenocarcinomas in TCGA high-throughput sequencing data

... In this study, we present a comprehensive screening for viruses in NGS data of nine common cancers in 1,007 patients, using The Cancer Genome Atlas (TCGA) data. TCGA is a joint project of the National Cancer In- stitute ... See full document

12

High efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing

High efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing

... relative expression levels of different miRNAs within a single sample also offers an important approach to identify functionally significant ...gene expression analysis by qRT- PCR and microarray ... See full document

13

Differential genome-wide profiling of alternative polyadenylation sites in nasopharyngeal carcinoma by high-throughput sequencing

Differential genome-wide profiling of alternative polyadenylation sites in nasopharyngeal carcinoma by high-throughput sequencing

... Differential usage of poly(A) between NPC and NNET Previous studies have demonstrated that most cancer cells [27] and cancer tissues [31, 32] tend to use short- ened 3’UTRs. In contrast to these results for primary ... See full document

11

A method for high throughput gene expression signature analysis

A method for high throughput gene expression signature analysis

... Gene expression signature analysis

A new method for cost-effective high-throughput gene expression signature analysis is ...transcriptional profiling has shown that ... See full document

6

Telomere and Proximal Sequence Analysis Using High-Throughput Sequencing Reads

Telomere and Proximal Sequence Analysis Using High-Throughput Sequencing Reads

... the expression TERRA, an essential long non-coding RNA, and to better characterize their transcription start ...ChIP-seq analysis in the human subtelomere found colocalization of CTCF and Cohesin directly ... See full document

174

Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS)

Introduction to transcriptome analysis using High Throughput Sequencing technologies (HTS)

... the expression values are similar between the ...low expression … as background correction methods for arrays are complicated when signal levels approach noise ... See full document

27

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

... Although the fluorescence during qRT-PCR is primarily determined by the starting abundance of a given cDNA template, its increase during the run is strongly affected by the amplification efficiency. When this efficiency ... See full document

9

Tools and best practices for retrotransposon analysis using high-throughput sequencing data

Tools and best practices for retrotransposon analysis using high-throughput sequencing data

... We also studied the specific case of TEtools, which quantifies TEs using randomly reported reads with Bow- tie or Bowtie2. However, this tool considers a list of TE sequences extracted from a genome or manually anno- ... See full document

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