[PDF] Top 20 The T-antigen-binding domain of the simian virus 40 core origin of replication.

... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document

... Topo I, bound either to T antigen or to a cellular protein in a replicative complex, is positioned in the core origin to take advantage of the clusters of Topo I sites in the AT segment [r] ... See full document

... Footprinting of the insertion mutants and the four pentanucleotide mutants strongly suggests that the early and late halves of the origin function as distinct entities in formation of th[r] ... See full document

... Because T antigen covers only the center palindrome of the origin at 37°C, the binding of T antigen to region I or of the positive regulatory protein Spl to the 21-bp repeats 13 could al[r] ... See full document

... Simian virus 40 SV40 large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication.. Molecular cloning: a laboratory manual, 2nd ed.[r] ... See full document

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document

... WT T antigen, especially at low protein ...ssDNA binding is not clear. One possibility is that in WT T antigen, the three residues, K446, R540, and T536, interfere in some way with the ... See full document

... the T-ag OBD with the SV40 origin remain to be ...through T-ag double hexamers assembled on the core ...a T-ag OBD hexamer and its interactions with ...red. T-ag OBD residue ... See full document

... drome domain for DNA unwinding, we generated mutant 31-bp DNAs with a single base-pair substitution at each site within the central ...31-bp origin DNA unwinding and binding assays of wild-type and ... See full document

... However, when chromatin was isolated from the variant 1103 19, which contains nine copies of the origin of DNA replication and lacks at least one and possibly two of the other high-affin[r] ... See full document

... DNA binding domain of large T antigen to amino acid residues 259 to ...this domain most likely begins after residue 301 and that the region between residues 501 and 550 is not ...this ... See full document

... small t helper function in SV40 transforma- tion and metabolic stabilization of p53 in complex with large ...large T actively contributes to phenotypic trans- formation by SV40, both during initiation and ... See full document

... of T-ag, shown in yellow, binds to site I (the J-domain is not ...the binding of the initial monomer, additional T-ag molecules are recruited to site I owing to the high affinity of the ... See full document

... the binding reaction (48) did not prevent RPA binding to the fusion proteins (data not ...of T antigen was then performed to define the site of interaction more ...to T- antigen ... See full document

... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document

... Specific binding to the unmethylated 1,229-base-pair fragment was not observed, even at higher T-antigen concentrations, nor was there binding to the smaller 222-base-pair plasmid fragme[r] ... See full document

... DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance complex, the ... See full document

... 3); T-ag assembly on this molecule was limited to hexamer formation ...of T-ag with the EP ...of T-ag hexamers and double ...specific binding events may explain, at least in part, assembly on ... See full document

... as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...large T antigen in the case of SV40 ... See full document

... all T-antigen mutants carry- ing mutations in the origin-binding domain which bind to DNA normally also structurally distort origin DNA normally ...minimal binding ... See full document