[PDF] Top 20 Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep protein.

... (29). Replication of AAV is dependent on the AAV nonstructural proteins (Rep); either Rep68 or Rep78 is the only AAV pro- tein required for replication in vitro (16, ...DNA ... See full document

... that Rep itself was probably not an activator and left open the question of how the p5 RBE could transactivate p19 in the context of the normal AAV ...transcription from the p5 promoter activated p19 by a ... See full document

... one Rep-DNA complex was formed on ch-19 target ...RBE sequence, but never on DNA lacking an RBE. Rep-DNA complexes involved a multimeric protein structure that spanned ...Rep68 protein ... See full document

... of Rep with the transcription template is not the mechanism of ...the Rep binding site using competitive, unlabeled oligonucleotides re- vealed that the Rep binding site spanned the TATA ...17-bp ... See full document

... the rep- lication complex around the circular template, a mechanism known as RCR (11, ...lytic replication as a mechanism for resolution of dimer intermedi- ates ...AAV replication, it is useful to ... See full document

... DNA replication in uninfected cell extracts with purified human DNA replication ...the Rep proteins, AAV DNA replication must involve cellular and/or adenovirus replication ... See full document

... by adeno-associated virus type 2 (AAV) requires coinfection with a helper virus, ...the replication machinery of the host cell performs AAV DNA ...herpesvirus replication ... See full document

... of rep ablates both inhibition of adeno- viral replication and p40 promoter ...Rep78 protein (26), we have recently demonstrated that an isolated sequence element from the ... See full document

... nonautonomous human parvoviruses in that they are dependent on helper functions supplied by other viruses or on genotoxic stimuli for conditions permissive for ...on human chromosome ...for ... See full document

... RBE element, we observed very strong Rep-mediated ...RBE element with the ITR-RBE sequence or the AAVS1-RBE elements may have increased the sensitivity to Rep-dependent ... See full document

... of human T-cell leukemia virus type 1 (21) and the ICP0 protein of HSV-1 which de- grades DNA-PKcs ...inhibiting protein phosphatase 2A activity (31, ...AAV replication as a model ... See full document

... amplified from the genomic DNA extracted from cells subcultured for 14 days and detected with an AAVS1-derived probe as described in the footnote to Table ...(D) Sequence analysis of ITR/AAVS1 ... See full document

... the Rep-dependent replication of the minimal p5 ...a Rep-expressing construct and with (or without) a plasmid encoding human ...p5D10 element could replicate in the presence of ... See full document

... E1a protein activates the AAV2 P5 promoter to express Rep proteins (26, 27), and this is a critical step in AAV2 ...DNA replication as efficiently as Ad ...the replication of the duplex AAV2 ... See full document

... AAV replication resulted in the identification of a single adenoviral helper protein ...AAV replication than that of ...identified from complexes purified from cells at 20 h ...AAV ... See full document

... strate from which AAV can be rescued (32, ...Epstein-Barr virus model system ...result from replication or recombination of the integrating DNA prior to or during in- ...AAV replication ... See full document

... 611 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Transfection of a pBR322-based, recombinant plasmid, pAV2, containing the entire adeno-associated virus AAV type 2 g[r] ... See full document

... expresses Rep from the wt p5 promoter and is deleted in the right-end AAV sequence, which contains the cap ORF ...cap sequence is not required for efficient site-specific integration of an ... See full document

... acid sequence LARGHSL, which corresponds to the C-terminal end of the open reading frame (ORF) for the spliced Rep ...The rep coding portion of each mutant plasmid was excised from M13mp18 by ... See full document

... To determine whether the p40 promoter also required noncontiguous upstream sequences, two deletions were constructed which removed the p5 promoter and part or all of Downloaded from http[r] ... See full document