[PDF] Top 20 Further characterization of the Friend murine leukemia virus reverse transcriptase-RNase H complex.
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Further characterization of the Friend murine leukemia virus reverse transcriptase-RNase H complex.
... 418 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The purified reverse transcriptase-RNase H complex from Friend murine leukemia virus consists of a single polypeptid[r] ... See full document
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Specific Cleavages by RNase H Facilitate Initiation of Plus-Strand RNA Synthesis by Moloney Murine Leukemia Virus
... verse transcriptase must bind the RNA strand of the hybrid near the PPT to generate the plus-strand ...several RNase H cleavages occur at specific sites in the PPT region both upstream and down- ... See full document
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Development of an In Vivo Assay To Identify Structural Determinants in Murine Leukemia Virus Reverse Transcriptase Important for Fidelity
... template-primer complex and is associated with alterations in the processivity and fidelity of RT (3, 6, ...the RNase H domain may in- fluence the rate of RT processivity and template switching, ... See full document
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Mutations in the RNase H Primer Grip Domain of Murine Leukemia Virus Reverse Transcriptase Decrease Efficiency and Accuracy of Plus-Strand DNA Transfer
... MLV RNase H primer grip domain plays a significant role in dealing with structural constraints in the template-primer complex intro- duced by A tracts or DNA-RNA ...initiate reverse ... See full document
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Structural Determinants of Murine Leukemia Virus Reverse Transcriptase That Affect the Frequency of Template Switching
... with virus generated with the wild-type RT, as well as V223I and Y598V mutant forms of RT, in the absence or presence of 1 mM ...4 h prior to infection, 4 h during infection, and 24 h ...could ... See full document
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Mutations of the RNase H C Helix of the Moloney Murine Leukemia Virus Reverse Transcriptase Reveal Defects in Polypurine Tract Recognition
... efficient RNase H activity upon the PPT-U3 substrate than upon a nonspecific substrate with respect to the WT ...occurs further up- stream, retention of the PPT and further upstream sequences ... See full document
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Replication Defect of Moloney Murine Leukemia Virus with a Mutant Reverse Transcriptase That Can Incorporate Ribonucleotides and Deoxyribonucleotides
... F155V virus to make full-length viral DNA was the selective inhibition of the mutant RT-F155V by ...inhibitors. Further, ribonucleotides might be incorporated into the prod- uct and serve as inhibitors for ... See full document
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Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.
... RNases H to degrade their genomic RNA present in RNA-DNA hybrid ...with RNase A in either low (TE) or high ...concentrations. RNase A treatment in high salt concentrations will digest only ... See full document
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Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity.
... A purified preparation of the expressed enzyme pRT250 reverse transcriptase consisted primarily of a 69-kilodalton protein that has normal levels of murine leukemia virus polymerase acti[r] ... See full document
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RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity.
... the RNase H ...the RNase H activity its prefer- ence for cleaving at particular sites within the tRNA or the ...the RNase H domain exhibits a loss of ...isolated RNase ... See full document
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Characterization of Intracellular Reverse Transcription Complexes of Moloney Murine Leukemia Virus
... 16 h postinfection, and nuclear extracts were prepared 7 and 16 h ...of reverse transcription, could be detected as a discrete peak at a density of ...1 h after ...7 h after infection ... See full document
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Mutations in the gag gene of Moloney murine leukemia virus: effects on production of virions and reverse transcriptase.
... To explore the effects of these mutations on the utilization of the gag-pol precursor, we have introduced these mutants into cells already releasing defective particles from an endogenou[r] ... See full document
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Reverse transcriptase of RNA tumor viruses. V. In vitro proteolysis of reverse transcriptase from avian myeloblastosis virus and isolation of a polypeptide manifesting only RNase H activity.
... 25, 1978 PROTEOLYSIS OF AMV REVERSE TRANSCRIPTASE sequences only present in subunit ,B; and iii a smaller polypeptide of molecular weight 24,000 was isolated, which contained only RNase [r] ... See full document
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RNA-primed initiation of Moloney murine leukemia virus plus strands by reverse transcriptase in vitro.
... Each reaction contained, in addition to the standard salts mixture, [a-32P]dATP, 2,000 U of M-MuLV reverse transcriptase per ml and, as sources of template and potential primers, P190 fr[r] ... See full document
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A 2.4-kilobase-pair fragment of the Friend murine leukemia virus genome contains the sequences responsible for friend murine leukemia virus-induced erythroleukemia.
... Since the Ampho isolate used in these studies does not cause leukemia, the genetic sequences responsible for the erythroproliferative disease caused by 5a25-H must be contained within th[r] ... See full document
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Molecular characterization of a neuropathogenic and nonerythroleukemogenic variant of Friend murine leukemia virus PVC-211.
... In addition to containing a single copy of the direct repeat, the ClaI-KpnI fragment of PVC-211 MuLV contains changes of eight nucleotides in the U3 region and two nucleotides in the R r[r] ... See full document
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Reverse transcriptase-associated RNase H activity. II. Inhibition by natural and synthetic RNA.
... Reaction mixtures for measuring R-MuLV DNA polymerase-associated RNase H activity contained 0.5 mM MnCI2, whereas those for meas- Downloaded from http://jvi.asm.org/ on November 10, 2019[r] ... See full document
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Developing and Evaluating Inhibitors against the RNase H Active Site of HIV-1 Reverse Transcriptase
... ABSTRACT We tested three compounds for their ability to inhibit the RNase H (RH) and polymerase activities of HIV-1 reverse transcriptase (RT). A high-resolu- tion crystal structure (2.2 Å) of ... See full document
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Subgenomic fragment of molecular cloned Friend murine leukemia virus DNA contains the gene(s) responsible for Friend murine leukemia virus-induced disease.
... Friend murine leukemia virus F-MuLV is a helper-independent, ecotropic, XC-positive, type C retrovirus derived from stocks of Friend virus complex F-MuLV plus spleen focus-forming virus [r] ... See full document
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RNase H Activity Is Required for High-Frequency Repeat Deletion during Moloney Murine Leukemia Virus Replication
... and RNase H activities of reverse transcriptase (RT) ...The RNase H activity, which is directed by the C-terminal one-third of Molo- ney murine leukemia ... See full document
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